Strain identifier

BacDive ID: 4385

Type strain: No

Species: Erwinia amylovora

Strain Designation: Ea1/79

Strain history: <- K. Geider; Ea1/79 <- W. Zeller

NCBI tax ID(s): 552 (species)

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General

@ref: 7282

BacDive-ID: 4385

DSM-Number: 17948

keywords: 16S sequence, Bacteria, mesophilic, plant pathogen

description: Erwinia amylovora Ea1/79 is a mesophilic plant pathogen that was isolated from Malus domestica, James Grieve.

NCBI tax id

  • NCBI tax id: 552
  • Matching level: species

strain history

  • @ref: 7282
  • history: <- K. Geider; Ea1/79 <- W. Zeller

doi: 10.13145/bacdive4385.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Erwiniaceae
  • genus: Erwinia
  • species: Erwinia amylovora
  • full scientific name: Erwinia amylovora (Burrill 1882) Winslow et al. 1920 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Micrococcus amylovorus

@ref: 7282

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Erwiniaceae

genus: Erwinia

species: Erwinia amylovora

full scientific name: Erwinia amylovora (Burrill 1882) Winslow et al. 1920 emend. Hauben et al. 1998

strain designation: Ea1/79

type strain: no

Culture and growth conditions

culture medium

  • @ref: 7282
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

  • @ref: 7282
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7282+--------+-+----+--+-

Isolation, sampling and environmental information

isolation

  • @ref: 7282
  • sample type: Malus domestica, James Grieve
  • host species: Malus domestica
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_717.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_502;98_576;99_717&stattab=map
  • Last taxonomy: Erwinia
  • 16S sequence: U80195
  • Sequence Identity:
  • Total samples: 2160
  • soil counts: 90
  • aquatic counts: 255
  • animal counts: 1537
  • plant counts: 278

Safety information

risk assessment

  • @ref: 7282
  • pathogenicity plant: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7282
  • description: Erwinia amylovora 16S ribosomal RNA gene, partial sequence
  • accession: U80195
  • length: 1456
  • database: ena
  • NCBI tax ID: 552

External links

@ref: 7282

culture collection no.: DSM 17948

straininfo link

  • @ref: 73876
  • straininfo: 297661

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1317549Site-directed and transposon-mediated mutagenesis with pfd-plasmids by electroporation of Erwinia amylovora and Escherichia coli cells.Metzger M, Bellemann P, Schwartz T, Geider KNucleic Acids Res10.1093/nar/20.9.22651992Blotting, Southern, DNA Transposable Elements, Electric Stimulation, Erwinia/*genetics/metabolism, Escherichia coli/*genetics, Galactose/metabolism, Mutagenesis, Insertional/*methods, *Mutagenesis, Site-Directed, *Plasmids
Enzymology2131100The rcsA gene from Erwinia amylovora: identification, nucleotide sequence, and regulation of exopolysaccharide biosynthesis.Bernhard F, Poetter K, Geider K, Coplin DLMol Plant Microbe Interact10.1094/mpmi-3-4291990Amino Acid Sequence, Bacterial Proteins/*genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial, Erwinia/*genetics/pathogenicity, Escherichia coli/genetics, *Escherichia coli Proteins, Gene Expression Regulation, Bacterial, *Genes, Bacterial, Genetic Complementation Test, Molecular Sequence Data, Mutagenesis, Site-Directed, Phenotype, Polysaccharides, Bacterial/*biosynthesis, Virulence/geneticsGenetics
Enzymology11085262Genes of Erwinia amylovora involved in yellow color formation and release of a low-molecular-weight compound during growth in the presence of copper ions.Zhang Y, Jock S, Geider KMol Gen Genet10.1007/s0043800002902000Amino Acid Sequence, Base Sequence, Carotenoids/genetics, Cell Division, Cloning, Molecular, Copper/*pharmacology, Drug Resistance, Microbial/genetics, Erwinia/*genetics, Gene Library, Genetic Complementation Test, Ions, Models, Genetic, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Open Reading Frames, Phenotype, Plasmids/genetics/metabolism, Sequence Analysis, DNA, Sodium-Potassium-Exchanging ATPase/genetics, Transformation, GeneticGenetics
Phylogeny22538467Differentiation of Erwinia amylovora and Erwinia pyrifoliae strains with single nucleotide polymorphisms and by synthesis of dihydrophenylalanine.Gehring I, Geider KCurr Microbiol10.1007/s00284-012-0116-52012Bacterial Proteins/genetics/metabolism, Bacterial Typing Techniques/*methods, Base Sequence, Cyclohexenes, Erwinia/*genetics/isolation & purification/*metabolism, Erwinia amylovora/*genetics/isolation & purification/metabolism, Molecular Sequence Data, Phenylalanine/*analogs & derivatives/biosynthesis, Phylogeny, Plant Diseases/*microbiology, Plants/microbiology, Polymerase Chain Reaction/*methods, *Polymorphism, Single NucleotideMetabolism

Reference

@idauthorscataloguedoi/urltitle
7282Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17948)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17948
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73876Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297661.1StrainInfo: A central database for resolving microbial strain identifiers