Strain identifier

BacDive ID: 438

Type strain: Yes

Species: Aestuariibacter salexigens

Strain Designation: JC2042

Culture col. no.: DSM 15300, KCTC 12042

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5834

BacDive-ID: 438

DSM-Number: 15300

keywords: motile, gram-negative, mesophilic, chemoheterotroph, halophilic, obligate aerobe, Bacteria, 16S sequence, genome sequence, rod-shaped, colony-forming

description: Aestuariibacter salexigens JC2042 is an obligate aerobe, halophilic, chemoheterotroph bacterium that forms circular colonies and was isolated from sediment sample of getbol.

strain history: <- J. Chun; JC2042

doi: 10.13145/bacdive438.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Aestuariibacter
  • species: Aestuariibacter salexigens
  • full scientific name: Aestuariibacter salexigens Yi et al. 2004

@ref: 5834

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Aestuariibacter

species: Aestuariibacter salexigens

full scientific name: Aestuariibacter salexigens Yi et al. 2004

strain designation: JC2042

type strain: yes

Morphology

cell morphology

  • @ref: 23286
  • gram stain: negative
  • cell length: 1.0-1.8 µm
  • cell width: 0.4-0.6 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

  • @ref: 23286
  • colony color: white, opaque
  • colony shape: circular
  • incubation period: 2 days
  • medium used: MA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5834BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf
23286CSY-3yes
23286Marine agar (MA)yes
23286SMMyes
34319Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)

culture temp

@refgrowthtypetemperaturerange
5834positivegrowth35mesophilic
23286positivegrowth20-40
23286positiveoptimum35mesophilic
34319positivegrowth30mesophilic

culture pH

@refabilitytypepH
23286positiveoptimum7-8
23286positivegrowth6-11

Physiology and metabolism

oxygen tolerance

  • @ref: 23286
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 23286
  • type: chemoheterotroph

spore formation

  • @ref: 23286
  • spore formation: no

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23286halophilicNaClnogrowth0 %
23286Sea Salts (S9883)positivegrowth1-10 %
23286Sea Salts (S9883)positiveoptimum2-6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2328627856acetamide-carbon source
2328616708adenine-carbon source
2328616150benzoate-carbon source
2328616947citrate-carbon source
2328617057cellobiose-carbon source
2328615824D-fructose-carbon source
2328617634D-glucose-carbon source
2328616024D-mannose-carbon source
2328616634raffinose-carbon source
2328665327D-xylose-carbon source
2328617268myo-inositol-carbon source
2328617561L-cysteine-carbon source
2328662345L-rhamnose-carbon source
2328617716lactose-carbon source
23286178242-propanol-carbon source
2328617814salicin-carbon source
2328630762salicylate-carbon source
2328617992sucrose-carbon source
2328626948thiamine-carbon source
232862509agar-degradation
2328658187alginate-degradation
23286casein-degradation
2328662968cellulose-degradation
2328617029chitin-degradation
23286egg yolk-degradation
2328630089acetate+carbon source
2328615428glycine+carbon source
2328615443inulin+carbon source
2328616467L-arginine+carbon source
2328615729L-ornithine+carbon source
23286506227N-acetylglucosamine+carbon source
2328616991dna+degradation
232864853esculin+degradation
232865291gelatin+degradation
2328628017starch+degradation
2328653426tween 80+degradation
2328617632nitrate+reduction
2328612936D-galactose+/-carbon source
2328616988D-ribose+/-carbon source
2328616236ethanol+/-carbon source
2328618019L-lysine+/-carbon source
2328630911sorbitol+/-carbon source
2328630031succinate+/-carbon source

metabolite production

@refChebi-IDmetaboliteproduction
2328653388polyhydroxybutyrateno
2328635581indoleno
2328616136hydrogen sulfideno
2328631624fluoresceinno

enzymes

@refvalueactivityec
23286acid phosphatase-3.1.3.2
23286alkaline phosphatase+3.1.3.1
23286alpha-chymotrypsin-3.4.21.1
23286alpha-fucosidase-3.2.1.51
23286alpha-galactosidase-3.2.1.22
23286alpha-glucosidase-3.2.1.20
23286alpha-mannosidase-3.2.1.24
23286arginine dihydrolase-3.5.3.6
23286beta-galactosidase-3.2.1.23
23286beta-glucosidase-3.2.1.21
23286beta-glucuronidase-3.2.1.31
23286catalase+1.11.1.6
23286cystine arylamidase-3.4.11.3
23286cytochrome oxidase+1.9.3.1
23286esterase (C 4)+
23286esterase lipase (C 8)+
23286leucine arylamidase+3.4.11.1
23286lipase (C 14)-
23286N-acetyl-beta-glucosaminidase-3.2.1.52
23286naphthol-AS-BI-phosphohydrolase+
23286trypsin+3.4.21.4
23286urease-3.5.1.5
23286valine arylamidase-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling datelatitudelongitudeisolation procedure
5834sediment sample of getbolKorea, Ganghwa islandRepublic of KoreaKORAsia
23286sediment sample of getbol, the Korean tidal flatGanghwa islandRepublic of KoreaKORAsiaAugust 200237.5922126.457diluted with sterilized artificial sea water

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Aquatic#Tidal flat

Safety information

risk assessment

  • @ref: 5834
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23286
  • description: Aestuariibacter salexigens strain JC2042 16S ribosomal RNA gene, partial sequence
  • accession: AY207502
  • length: 1456
  • database: nuccore
  • NCBI tax ID: 226010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aestuariibacter salexigens DSM 15300GCA_000429145scaffoldncbi1120953
66792Aestuariibacter salexigens DSM 153001120953.3wgspatric1120953
66792Aestuariibacter salexigens DSM 153002524614540permanentimg1120953

GC content

  • @ref: 23286
  • GC-content: 48.00
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5834

culture collection no.: DSM 15300, KCTC 12042

straininfo link

@refpassport
20218http://www.straininfo.net/strains/377565
20218http://www.straininfo.net/strains/377568

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5834Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15300)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15300
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23286Hana Yi, Kyung Sook Bae, Jongsik Chun10.1099/ijs.0.02798-0Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodiiIJSEM 54: 571-576 2004
34319Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6033
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)