Strain identifier

BacDive ID: 4371

Type strain: Yes

Species: Enterobacter cloacae subsp. dissolvens

Strain Designation: W-4, 8122-86

Strain history: CIP <- 1998, D. Brenner, CDC: strain 8122-86 <- M.P. Starr <- NRRL B-154 <- E. Waldes: strain W-4, Phytomonas dissolvens <- H. Rosen

NCBI tax ID(s): 69219 (subspecies)

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General

@ref: 6555

BacDive-ID: 4371

DSM-Number: 16657

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Enterobacter cloacae subsp. dissolvens W-4 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from maize plant.

NCBI tax id

  • NCBI tax id: 69219
  • Matching level: subspecies

strain history

@refhistory
6555<- ATCC <- M. P. Starr <- NRRL <- E. L. Waldee, Iowa State College, USA; W-4
67770ATCC 23373 <-- M. P. Starr ICPB ED 1-5 <-- NRRL B-154 <-- E. Waldee W-4.
118314CIP <- 1998, D. Brenner, CDC: strain 8122-86 <- M.P. Starr <- NRRL B-154 <- E. Waldes: strain W-4, Phytomonas dissolvens <- H. Rosen

doi: 10.13145/bacdive4371.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter cloacae subsp. dissolvens
  • full scientific name: Enterobacter cloacae subsp. dissolvens (Rosen 1922) Hoffmann et al. 2005

  • synonyms

    @refsynonym
    20215Pseudomonas dissolvens
    20215Erwinia dissolvens
    20215Enterobacter dissolvens

@ref: 6555

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter cloacae subsp. dissolvens

full scientific name: Enterobacter cloacae subsp. dissolvens (Rosen 1922) Hoffmann et al. 2005

strain designation: W-4, 8122-86

type strain: yes

Morphology

cell morphology

  • @ref: 118314
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6555NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39321MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
118314CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
6555positivegrowth28mesophilic
39321positivegrowth30mesophilic
48160positivegrowth30mesophilic
67770positivegrowth37mesophilic
118314positivegrowth10-41
118314nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
48160aerobe
118314facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose+fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose+fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate+assimilation16947
68368ornithine+degradation18257
68368lysine-degradation25094
68368arginine+hydrolysis29016
118314mannitol+fermentation29864
118314citrate+carbon source16947
118314glucose+fermentation17234
118314lactose+fermentation17716
118314nitrate+reduction17632
118314nitrite-reduction16301
118314malonate+assimilation15792
118314sodium thiosulfate-builds gas from132112
118314glucose+degradation17234

antibiotic resistance

  • @ref: 118314
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11831435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11831415688acetoin+
11831417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
118314oxidase-
118314beta-galactosidase+3.2.1.23
118314alcohol dehydrogenase+1.1.1.1
118314gelatinase-
118314catalase+1.11.1.6
118314lysine decarboxylase-4.1.1.18
118314ornithine decarboxylase+4.1.1.17
118314phenylalanine ammonia-lyase-4.3.1.24
118314tryptophan deaminase-
118314urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118314-+++++----++-+-+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
6555++-++----+-+++++++++-
6555++-++----+-+++++++++-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118314+--+++---++++-+-+++-++-++++++/-+++--++/---++/-+/-------+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118314+++++-++++++-+++-+++++++-------++++-+--+-+-++----+++-++++--+++----------+--+--+++-+---+-++++++++---

Isolation, sampling and environmental information

isolation

@refsample type
6555maize plant
48160Zea mays
118314Corn

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_267.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_8;99_267&stattab=map
  • Last taxonomy: Salmonella
  • 16S sequence: Z96079
  • Sequence Identity:
  • Total samples: 2995
  • soil counts: 102
  • aquatic counts: 163
  • animal counts: 2506
  • plant counts: 224

Safety information

risk assessment

@refpathogenicity humanpathogenicity plantbiosafety levelbiosafety level comment
6555yes, in single casesyes1Risk group (German classification)
1183142Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterobacter dissolvens partial 16S rRNA gene, strain ATCC 23373AJ417485420ena69219
20218Enterobacter cloacae subsp. dissolvens strain ATCC 23373 16S ribosomal RNA gene, partial sequenceHQ6518411507ena69219
20218Enterobacter cloacae subsp. cloacae strain DSMZ 16657 16S ribosomal RNA gene, partial sequenceKF5162561347ena336306
20218Enterobacter cloacae subsp. dissolvens strain LMG 2683 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU078570991ena69219
20218Enterobacter dissolvens LMG 2683 16S ribosomal RNAZ960791495ena69219

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter cloacae subsp. dissolvens strain ATCC 2337369219.9wgspatric69219
67770Enterobacter cloacae subsp. dissolvens ATCC 23373GCA_009660455contigncbi69219

GC content

  • @ref: 67770
  • GC-content: 54.5-55.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno97.858no
anaerobicno95.841no
halophileno87.35no
spore-formingno92.498no
glucose-utilyes95.685no
thermophileno99.629no
flagellatedno55.623no
motileyes89.581no
aerobicyes81.295yes
glucose-fermentyes94.511no

External links

@ref: 6555

culture collection no.: DSM 16657, ATCC 23373, CIP 105586, ICMP 1570, NCPPB 1850, NRRL B-154, CCUG 25230, LMG 2683, JCM 6049, ICPB ED 105, ICPB 1570

straininfo link

  • @ref: 73862
  • straininfo: 2213

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15900966Reassignment of enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei.Hoffmann H, Stindl S, Ludwig W, Stumpf A, Mehlen A, Heesemann J, Monget D, Schleifer KH, Roggenkamp ASyst Appl Microbiol10.1016/j.syapm.2004.12.0102005Bacterial Typing Techniques, DNA, Bacterial/chemistry/*genetics, DNA, Ribosomal/chemistry, Enterobacter/*classification/physiology, Esculin/metabolism, Genes, rRNA, Molecular Sequence Data, *Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Transition TemperatureGenetics
Phylogeny16156121A toxaphene-degrading bacterium related to Enterobacter cloacae, strain D1 isolated from aged contaminated soil in Nicaragua.Lacayo-Romero M, Quillaguaman J, van Bavel B, Mattiasson BSyst Appl Microbiol10.1016/j.syapm.2005.03.0152005Biodegradation, Environmental, DNA, Ribosomal/analysis, Enterobacter cloacae/*classification/metabolism, Nicaragua, Phylogeny, *Soil Microbiology, Soil Pollutants/metabolism, Toxaphene/*metabolismMetabolism
Phylogeny19648330Mangrovibacter plantisponsor gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka).Rameshkumar N, Lang E, Nair SInt J Syst Evol Microbiol10.1099/ijs.0.008292-02009Bacterial Proteins/genetics, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Enterobacteriaceae/*classification/genetics/*isolation & purification/metabolism, Fatty Acids/metabolism, Molecular Sequence Data, *Nitrogen Fixation, Oryza/*microbiology, Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/geneticsMetabolism
Genetics23209248Draft genome sequence of a novel bacterial strain, LSJC7, belonging to the family Enterobacteriaceae with dual resistance to arsenic and tetracycline.Su J, Ye J, Zhu YJ Bacteriol10.1128/JB.01652-122012Anti-Bacterial Agents/*pharmacology, Antimony, Arsenic/*pharmacology, Bacterial Typing Techniques, DNA, Bacterial/genetics, Drug Resistance, Multiple, Bacterial, Enterobacteriaceae/drug effects/*genetics/isolation & purification, *Genome, Bacterial, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tetracycline/*pharmacologyPhylogeny
Phylogeny23759866Enterobacter siamensis sp. nov., a transglutaminase-producing bacterium isolated from seafood processing wastewater in Thailand.Khunthongpan S, Bourneow C, H-Kittikun A, Tanasupawat S, Benjakul S, Sumpavapol PJ Gen Appl Microbiol10.2323/jgam.59.1352013Aerobiosis, Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Enterobacter/*classification/*enzymology/genetics/isolation & purification, Fatty Acids/analysis, Industrial Waste, Microscopy, Electron, Scanning, Microscopy, Phase-Contrast, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood, Sequence Analysis, DNA, Thailand, Transglutaminases/*metabolism, Waste Water/*microbiologyEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6555Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16657)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16657
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39321Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17621
48160Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 25230)https://www.ccug.se/strain?id=25230
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73862Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2213.1StrainInfo: A central database for resolving microbial strain identifiers
118314Curators of the CIPCollection of Institut Pasteur (CIP 105586)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105586