Strain identifier

BacDive ID: 4368

Type strain: Yes

Species: Enterobacter hormaechei

Strain Designation: 992-77

Strain history: CIP <- 1990, ATCC <- C.M. O'Hara, CDC: strain 992-77 <- San Francisco Gen. Hosp., USA

NCBI tax ID(s): 888063 (strain), 158836 (species)

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General

@ref: 4546

BacDive-ID: 4368

DSM-Number: 12409

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Enterobacter hormaechei 992-77 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that was isolated from sputum of a male patient.

NCBI tax id

NCBI tax idMatching level
888063strain
158836species

strain history

@refhistory
4546<- CIP <- ATCC <- CDC <- San Francisco General Hospital
119971CIP <- 1990, ATCC <- C.M. O'Hara, CDC: strain 992-77 <- San Francisco Gen. Hosp., USA

doi: 10.13145/bacdive4368.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter hormaechei
  • full scientific name: Enterobacter hormaechei O'Hara et al. 1990

@ref: 4546

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter hormaechei subsp. hormaechei

full scientific name: Enterobacter hormaechei subsp. hormaechei (O'Hara et al. 1990) Hoffmann et al. 2016

strain designation: 992-77

type strain: yes

Morphology

cell morphology

  • @ref: 119971
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4546COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4546CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
41955MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119971CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4546positivegrowth30mesophilic
41955positivegrowth30mesophilic
119971positivegrowth10-41
119971nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119971
  • oxygen tolerance: facultative anaerobe

compound production

  • @ref: 4546
  • compound: beta lactamase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
11997129864mannitol+fermentation
11997116947citrate+carbon source
11997117234glucose+fermentation
11997117716lactose-fermentation
11997117632nitrate+reduction
11997116301nitrite+reduction
11997115792malonate+assimilation
119971132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 119971
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836835581indoleno
6836816136hydrogen sulfideno
11997135581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836835581indole-
11997115688acetoin-
11997117234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
119971oxidase-
119971beta-galactosidase+3.2.1.23
119971alcohol dehydrogenase+1.1.1.1
119971gelatinase-
119971catalase+1.11.1.6
119971lysine decarboxylase-4.1.1.18
119971ornithine decarboxylase+4.1.1.17
119971phenylalanine ammonia-lyase-4.3.1.24
119971tryptophan deaminase-
119971urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119971-+---+-++-++-+-+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
4546++-++----++/-++--+++++-
4546++-++------++--++-++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119971+++++--+-+++-+-++++++++++----+-+-+++---++++++---+++--++++--++++--+---------+---++-+--++--+++++++-++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4546sputum of a male patientCalifornia, San FranciscoUSAUSANorth America
48661Human bloodHonoluluUSAUSANorth America
119971Human, SputumSan Francisco, CaliforniaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body Product#Fluids#Sputum
#Host#Human#Male

taxonmaps

  • @ref: 69479
  • File name: preview.99_66.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_66&stattab=map
  • Last taxonomy: Enterobacterales
  • 16S sequence: KF516255
  • Sequence Identity:
  • Total samples: 14221
  • soil counts: 575
  • aquatic counts: 888
  • animal counts: 11838
  • plant counts: 920

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
4546yesyes2Risk group (German classification)
1199712Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterobacter cloacae partial 16S rRNA gene, strain ATCC 49162TAJ417450422ena550
20218Enterobacter hormaechei subsp. hormaechei strain DSMZ 12409 16S ribosomal RNA gene, partial sequenceKF5162551347ena301105
4546Enterobacter hormaechei partial 16S rRNA gene, strain CIP 103441AJ5083021368ena888063

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter hormaechei ATCC 49162GCA_001875655scaffoldncbi888063
66792Enterobacter hormaechei ATCC 49162GCA_000213995scaffoldncbi888063
66792Enterobacter hormaechei ATCC 49162888063.3wgspatric888063
66792Enterobacter hormaechei ATCC 49162888063.8wgspatric888063
66792Enterobacter hormaechei strain FDAARGOS 1433158836.1173completepatric158836
66792Enterobacter hormaechei ATCC 49162651324029draftimg888063
66792Enterobacter hormaechei ATCC 491622916426963draftimg888063

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.22no
gram-positiveno97.913no
anaerobicno96.643no
aerobicyes85.247no
halophileno87.02no
spore-formingno92.222no
thermophileno99.003yes
glucose-utilyes94.834no
flagellatedno59.678no
glucose-fermentyes92.775yes

External links

@ref: 4546

culture collection no.: DSM 12409, ATCC 49162, CCUG 26642, CCUG 27126, CIP 103441, CCM 8620

straininfo link

  • @ref: 73859
  • straininfo: 92993

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny2778068Enterobacter hormaechei, a new species of the family Enterobacteriaceae formerly known as enteric group 75.O'Hara CM, Steigerwalt AG, Hill BC, Farmer JJ 3rd, Fanning GR, Brenner DJJ Clin Microbiol10.1128/jcm.27.9.2046-2049.19891989Adolescent, Adult, Aged, Aged, 80 and over, Anti-Bacterial Agents/pharmacology, Child, DNA, Bacterial/analysis, Enterobacter/*classification/drug effects/genetics, Enterobacteriaceae/*classification, Enterobacteriaceae Infections/blood/*microbiology, Female, Humans, Infant, Newborn, Male, Middle Aged, Nucleic Acid Hybridization, Sputum/microbiology, *Terminology as Topic, Wound Infection/*microbiologyPathogenicity
Phylogeny24824638Enterobacter xiangfangensis sp. nov., isolated from Chinese traditional sourdough, and reclassification of Enterobacter sacchari Zhu et al. 2013 as Kosakonia sacchari comb. nov.Gu CT, Li CY, Yang LJ, Huo GCInt J Syst Evol Microbiol10.1099/ijs.0.064709-02014Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Enterobacter/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Fermentation, *Food Microbiology, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNABiotechnology
29472760Genetic Diversity and Functional Analysis of Sigma Factors in Enterobacter cloacae Complex Resourced From Various Niche.Nazir F, Ibrahim M, Zaman G, Hussain A, Yar AM, Bo ZEvol Bioinform Online10.1177/11769343187548782018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4546Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12409)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12409
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41955Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15239
48661Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27126)https://www.ccug.se/strain?id=27126
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73859Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92993.1StrainInfo: A central database for resolving microbial strain identifiers
119971Curators of the CIPCollection of Institut Pasteur (CIP 103441)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103441