Strain identifier

BacDive ID: 4363

Type strain: No

Species: Enterobacter cloacae

Strain Designation: A-8

Strain history: <- CECT <- CIP <- R. Mercier, bioMérieux, La Balme-les-Grottes, France <- ATCC <- Dept. Medical Microbiol, Stanford Univ.

NCBI tax ID(s): 550 (species)

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General

@ref: 18242

BacDive-ID: 4363

DSM-Number: 26481

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile, antibiotic resistance

description: Enterobacter cloacae A-8 is a mesophilic, motile bacterium that has multiple antibiotic resistances.

NCBI tax id

  • NCBI tax id: 550
  • Matching level: species

strain history

  • @ref: 18242
  • history: <- CECT <- CIP <- R. Mercier, bioMérieux, La Balme-les-Grottes, France <- ATCC <- Dept. Medical Microbiol, Stanford Univ.

doi: 10.13145/bacdive4363.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter cloacae
  • full scientific name: Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Cloaca cloacae
    20215Bacillus cloacae
    20215Bacterium cloacae

@ref: 18242

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter cloacae

full scientific name: Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960

strain designation: A-8

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes97.133
6948099.976negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18242NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
18242TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18242COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
18242positivegrowth28mesophilic
18242positivegrowth37mesophilic

Physiology and metabolism

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
18242Mueller-Hinton Agar301aerob802638-403018284042403022-242628000363616-183036818302222-24380034000032-34
18242Mueller-Hinton Agar30aerob6028-30403218284244-4640-423230-32262866-804040163236018-2028-30222640-420038-406-800034

spore formation

@refspore formationconfidence
69481no100
69480no99.901

compound production

@refcompound
18242cephalosporinase
18242beta lactamase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
6837418257ornithine+degradation
6837429016arginine+hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate+assimilation
6837427897tryptophan-energy source
6837417634D-glucose+builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose+builds acid from
6837462345L-rhamnose+builds acid from
6837417268myo-inositol+builds acid from
6837417057cellobiose+builds acid from
6837430911sorbitol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837435581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase+3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase+3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
18242++-++------+++++++++-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
51730++-------+-+---+--++----+++++---

Isolation, sampling and environmental information

isolation

  • @ref: 18242
  • country: USA
  • origin.country: USA
  • continent: North America

taxonmaps

  • @ref: 69479
  • File name: preview.99_120.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_120&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: KF516238
  • Sequence Identity:
  • Total samples: 13334
  • soil counts: 615
  • aquatic counts: 1353
  • animal counts: 10132
  • plant counts: 1234

Safety information

risk assessment

  • @ref: 18242
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Enterobacter cloacae subsp. cloacae strain ATCC 23355 16S ribosomal RNA gene, partial sequence
  • accession: KF516238
  • length: 1347
  • database: ena
  • NCBI tax ID: 336306

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter cloacae strain DSM 26481550.2783completepatric550
66792Enterobacter cloacae DSM 26481GCA_013375935chromosomencbi550

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno97.822no
anaerobicno96.404no
halophileno84.237no
spore-formingno91.908no
glucose-utilyes95.304no
thermophileno99.237yes
aerobicyes81.312no
motileyes89.15no
flagellatedno64.522no
glucose-fermentyes93.998yes

External links

@ref: 18242

culture collection no.: DSM 26481, ATCC 23355, CCUG 33777, CECT 5075, CIP 103550, WDCM 00082

straininfo link

  • @ref: 73854
  • straininfo: 38005

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1955391Localization of cephalosporinase in Enterobacter cloacae by immunocytochemical examination.Ishii Y, Ichikawa M, Yamaguchi K, Takano K, Inoue MJ Antibiot (Tokyo)10.7164/antibiotics.44.10881991Anti-Bacterial Agents/pharmacology, Blotting, Western, Cefoxitin/*pharmacology, Cephalosporinase/*analysis/biosynthesis, Drug Resistance, Microbial/physiology, Enterobacter cloacae/chemistry/drug effects/*enzymology/ultrastructure, Enzyme Induction, Immunohistochemistry, Microbial Sensitivity Tests, Microscopy, ImmunoelectronPathogenicity
Enzymology6378089Novel method for studying the public health significance of macroinvertebrates occurring in potable water.Levy RV, Cheetham RD, Davis J, Winer G, Hart FLAppl Environ Microbiol10.1128/aem.47.5.889-894.19841984Adsorption, Animals, *Chlorine, Crustacea/*microbiology, *Disinfectants, Enterobacter/physiology, *Enterobacteriaceae/physiology, Escherichia coli/physiology, Kinetics, Public Health, *Water Microbiology, Water Pollution, *Water Supply
Pathogenicity8843303Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test.White RL, Burgess DS, Manduru M, Bosso JAAntimicrob Agents Chemother10.1128/AAC.40.8.19141996Bacteria/*drug effects, Cefepime, Ceftazidime/pharmacology, Cephalosporins/pharmacology, Ciprofloxacin/pharmacology, *Drug Synergism, Drug Therapy, Combination/*pharmacology, Enterobacter cloacae/drug effects, Escherichia coli/drug effects, Microbial Sensitivity Tests/*methods, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effects, Tobramycin/pharmacology
Enzymology17258829Bioassay for determination of fosmidomycin in plasma and urine: application for pharmacokinetic dose optimisation.Cheoymang A, Hudchinton D, Kioy D, Na-Bangchang KJ Microbiol Methods10.1016/j.mimet.2006.11.0182007Anti-Bacterial Agents/pharmacology, Antimalarials/blood/*pharmacokinetics/urine, Biological Assay/*methods, Calibration, Clindamycin/blood/pharmacokinetics/urine, Dose-Response Relationship, Drug, Enterobacter cloacae/drug effects/metabolism, Fosfomycin/*analogs & derivatives/blood/pharmacokinetics/urine, Humans, Reproducibility of Results, Sensitivity and SpecificityPathogenicity
Pathogenicity20188200A high-throughput colorimetric-based bioassay method for determination of fosmidomycin in plasma and urine and application for pharmacokinetic study.Cheoymang A, Na-Bangchang KJ Pharmacol Toxicol Methods10.1016/j.vascn.2010.02.0122010Biological Assay/*methods, Colorimetry/methods, Enterobacter cloacae/drug effects, Fosfomycin/*analogs & derivatives/blood/pharmacokinetics/urine, Humans, Malaria, Falciparum/blood/urineEnzymology
Pathogenicity27930849Searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.Moreira CS, Silva AC, Novais JS, Sa Figueiredo AM, Ferreira VF, da Rocha DR, Castro HCJ Appl Microbiol10.1111/jam.133692017Anti-Bacterial Agents/*pharmacology, Biofilms/*drug effects, Enterobacter cloacae, Enterococcus faecalis/drug effects, Escherichia coli/drug effects, Gram-Positive Bacteria/drug effects, Humans, Klebsiella pneumoniae/drug effects, Methicillin-Resistant Staphylococcus aureus/*drug effects, Microbial Sensitivity Tests, Naphthoquinones/chemistry/*pharmacology, Proteus mirabilis/drug effects, Pseudomonas aeruginosa/drug effects, Staphylococcus/drug effects, Vancomycin
Pathogenicity29550501Antibacterial naphthoquinone derivatives targeting resistant strain Gram-negative bacteria in biofilms.Novais JS, Moreira CS, Silva ACJA, Loureiro RS, Sa Figueiredo AM, Ferreira VF, Castro HC, da Rocha DRMicrob Pathog10.1016/j.micpath.2018.03.0242018Anti-Bacterial Agents/chemical synthesis/chemistry/*pharmacology/toxicity, Biofilms/*drug effects, Ciprofloxacin/pharmacology, Erythrocytes/drug effects, Gram-Negative Bacteria/*drug effects, Gram-Positive Bacteria/drug effects, Humans, Materials Testing, Microbial Sensitivity Tests, Naphthoquinones/chemical synthesis/chemistry/*pharmacology/toxicity
30732006First Report of Enterobacter Bulb Decay of Onions Caused by Enterobacter cloacae in New York.Zaid AM, Bonasera JM, Beer SVPlant Dis10.1094/PDIS-05-11-03752011
30764199First Report of Enterobacter cloacae Causing Onion Bulb Rot in the Columbia Basin of Washington State.Schroeder BK, du Toit LJ, Schwartz HFPlant Dis10.1094/PDIS-93-3-0323A2009
35255107Antibacterial activities of two potential peptides extracted from Polistes wattii Cameron, 1900 (Vespidae: Polistinae) wasp venom collected at Eastern Province, Saudi Arabia.A Al-Shammery K, Hozzein WNPLoS One10.1371/journal.pone.02640352022Animals, Anti-Bacterial Agents/pharmacology, Bacteria, Microbial Sensitivity Tests, Peptides/pharmacology, Saudi Arabia, *Staphylococcal Infections, Staphylococcus aureus, Wasp Venoms/chemistry/pharmacology, *Wasps

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18242Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26481)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26481
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
51730Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33777)https://www.ccug.se/strain?id=33777
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
73854Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38005.1StrainInfo: A central database for resolving microbial strain identifiers