Strain identifier
BacDive ID: 4356
Type strain:
Species: Edwardsiella ictaluri
Strain Designation: GA 77-52, 1976-78
Strain history: CIP <- 1981, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 1976-78 <- Auburn Univ., USA: strain GA 77-52
NCBI tax ID(s): 1027360 (strain), 67780 (species)
General
@ref: 5120
BacDive-ID: 4356
DSM-Number: 13697
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen
description: Edwardsiella ictaluri GA 77-52 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that was isolated from channel catfish, Ictalurus punctatus.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1027360 | strain |
67780 | species |
strain history
@ref | history |
---|---|
5120 | <- ATCC <- J. P. Hawke; GA 77-52 |
67770 | R. Sakazaki 146 <-- ATCC 33202 <-- J. P. Hawke GA77-52. |
120041 | CIP <- 1981, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 1976-78 <- Auburn Univ., USA: strain GA 77-52 |
doi: 10.13145/bacdive4356.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Hafniaceae
- genus: Edwardsiella
- species: Edwardsiella ictaluri
- full scientific name: Edwardsiella ictaluri Hawke et al. 1981
@ref: 5120
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Hafniaceae
genus: Edwardsiella
species: Edwardsiella ictaluri
full scientific name: Edwardsiella ictaluri Hawke et al. 1981
strain designation: GA 77-52, 1976-78
type strain: yes
Morphology
cell morphology
- @ref: 120041
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5120 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
5120 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
37629 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
120041 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 | |
120041 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5120 | positive | growth | 28 | mesophilic |
37629 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
120041 | positive | growth | 25-37 | mesophilic |
120041 | no | growth | 15 | psychrophilic |
120041 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120041
- oxygen tolerance: facultative anaerobe
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | - | builds acid from | 17814 |
68371 | esculin | - | builds acid from | 4853 |
68371 | arbutin | - | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | + | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68368 | L-arabinose | - | fermentation | 30849 |
68368 | amygdalin | - | fermentation | 27613 |
68368 | melibiose | - | fermentation | 28053 |
68368 | sucrose | - | fermentation | 17992 |
68368 | L-rhamnose | - | fermentation | 62345 |
68368 | sorbitol | - | fermentation | 30911 |
68368 | myo-inositol | - | fermentation | 17268 |
68368 | D-mannitol | - | fermentation | 16899 |
68368 | D-glucose | + | fermentation | 17634 |
68368 | gelatin | - | hydrolysis | 5291 |
68368 | tryptophan | - | energy source | 27897 |
68368 | urea | - | hydrolysis | 16199 |
68368 | citrate | - | assimilation | 16947 |
68368 | ornithine | - | degradation | 18257 |
68368 | lysine | + | degradation | 25094 |
68368 | arginine | - | hydrolysis | 29016 |
120041 | nitrate | + | reduction | 17632 |
120041 | nitrite | - | reduction | 16301 |
120041 | nitrate | + | respiration | 17632 |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
120041 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - | |
120041 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68368 | cytochrome oxidase | - | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
120041 | oxidase | - | |
120041 | alcohol dehydrogenase | - | 1.1.1.1 |
120041 | catalase | + | 1.11.1.6 |
120041 | lysine decarboxylase | + | 4.1.1.18 |
120041 | ornithine decarboxylase | - | 4.1.1.17 |
120041 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120041 | - | + | + | + | - | + | - | - | + | - | + | + | - | - | - | - | - | + | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5120 | - | - | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120041 | +/- | - | - | +/- | + | +/- | - | - | - | + | + | + | + | - | - | + | - | + | - | - | - | + | - | - | - | - | - | + | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | +/- | - | - | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|---|
5120 | channel catfish, Ictalurus punctatus | Ictalurus punctatus | Georgia | USA | USA | North America | |
46881 | Channel catfish | ||||||
67770 | Channel catfish (Ictalurus punctatus) | Ictalurus punctatus | |||||
120041 | Animal, Catfish, enteric septicemia | Georgia | United States of America | USA | North America | 1976 |
isolation source categories
Cat1 | Cat2 |
---|---|
#Environmental | #Aquatic |
#Host | #Fishes |
taxonmaps
- @ref: 69479
- File name: preview.99_47.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_38;97_40;98_44;99_47&stattab=map
- Last taxonomy: Edwardsiella
- 16S sequence: AB050826
- Sequence Identity:
- Total samples: 22716
- soil counts: 509
- aquatic counts: 2305
- animal counts: 19360
- plant counts: 542
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
5120 | yes | yes | 2 | Risk group (German classification) |
120041 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Edwardsiella ictaluri ATCC 33202 16S ribosomal RNA gene, partial sequence; short 16S-23S intergenic spacer and tRNA-Glu gene, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY706738 | 673 | ena | 1027360 |
20218 | Edwardsiella ictaluri gene for 16S rRNA, partial sequence | AB050826 | 1466 | ena | 1027360 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Edwardsiella ictaluri ATCC 33202 | 1027360.4 | wgs | patric | 1027360 |
66792 | Edwardsiella ictaluri strain NCTC12122 | 67780.22 | wgs | patric | 67780 |
66792 | Edwardsiella ictaluri ATCC 33202 | 2547132403 | draft | img | 1027360 |
66792 | Edwardsiella ictaluri NCTC 12122 | 2850123092 | draft | img | 67780 |
67770 | Edwardsiella ictaluri ATCC 33202 | GCA_000264785 | contig | ncbi | 1027360 |
67770 | Edwardsiella ictaluri NCTC12122 | GCA_900447225 | contig | ncbi | 67780 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 90.295 | no |
flagellated | yes | 56.267 | no |
gram-positive | no | 98.673 | no |
anaerobic | no | 79.822 | no |
aerobic | yes | 51.558 | no |
halophile | no | 90.57 | no |
spore-forming | no | 94.901 | no |
thermophile | no | 97.549 | yes |
glucose-util | yes | 93.23 | no |
glucose-ferment | yes | 92.3 | yes |
External links
@ref: 5120
culture collection no.: CCUG 18764, CECT 885, DSM 13697, ATCC 33202, CDC 1976-78, JCM 16934, JCM 1680, CGMCC 1.1873, CIP 81.96, KCTC 12264, LMG 7860, NBRC 105724, NCIMB 13272, NCTC 12122
straininfo link
- @ref: 73847
- straininfo: 389450
literature
topic | Pubmed-ID | title | authors | journal | year | mesh | topic2 | DOI |
---|---|---|---|---|---|---|---|---|
Cultivation | 1689127 | Outer membrane protein profiles of Edwardsiella ictaluri from fish. | Newton JC, Blevins WT, Wilt GR, Wolfe LG | Am J Vet Res | 1990 | Animals, Bacterial Outer Membrane Proteins/*analysis, Bacteriological Techniques/veterinary, Catfishes/*microbiology, Culture Media, Electrophoresis, Polyacrylamide Gel, Enterobacteriaceae/*analysis/growth & development, Fishes/microbiology, Ictaluridae/*microbiology, Molecular Weight, Sarcosine/analogs & derivatives, Time Factors | Enzymology | |
Enzymology | 3247906 | Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri. | Newton JC, Bird RC, Blevins WT, Wilt GR, Wolfe LG | Am J Vet Res | 1988 | Animals, Cloning, Molecular, DNA, Bacterial/analysis/genetics/*isolation & purification, Electrophoresis, Agar Gel, Enterobacteriaceae/*genetics/pathogenicity, Fishes, Ictaluridae, *Plasmids, Restriction Mapping, Virulence | Phylogeny | |
Enzymology | 22017642 | Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture. | Li X, Wu H, Zhang M, Liang S, Xiao J, Wang Q, Liu Q, Zhang Y | Lett Appl Microbiol | 2011 | *Aeromonas hydrophila, Animals, Antigens, Bacterial/*immunology, Aquaculture, Bacterial Infections/immunology/prevention & control/veterinary, Bacterial Vaccines/*immunology, *Edwardsiella, Fish Diseases/immunology/prevention & control, Glyceraldehyde-3-Phosphate Dehydrogenases/*immunology, Vaccines, Synthetic/immunology, *Vibrio, Zebrafish | 10.1111/j.1472-765X.2011.03164.x | |
Genetics | 24062549 | Comparison of Vietnamese and US isolates of Edwardsiella ictaluri. | Rogge ML, Dubytska L, Jung TS, Wiles J, Elkamel AA, Rennhoff A, Oanh DT, Thune RL | Dis Aquat Organ | 2013 | Animals, Anti-Bacterial Agents/pharmacology, Aquaculture, DNA Fingerprinting, Drug Resistance, Bacterial, Edwardsiella ictaluri/drug effects/*genetics/pathogenicity, Enterobacteriaceae Infections/epidemiology/microbiology/*veterinary, Fish Diseases/epidemiology/*microbiology, Gene Expression Regulation, Bacterial, Genome, Bacterial, *Ictaluridae, Plasmids/genetics, Specific Pathogen-Free Organisms, United States, Vietnam/epidemiology, Virulence | Pathogenicity | 10.3354/dao02620 |
24293714 | Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151. | Yang Q, Han Y, Tinh NT, Hien NT, Bossier P | Indian J Microbiol | 2012 | 10.1007/s12088-012-0312-9 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5120 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13697) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13697 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37629 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/11263 | ||||
46881 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 18764) | https://www.ccug.se/strain?id=18764 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
73847 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID389450.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120041 | Curators of the CIP | Collection of Institut Pasteur (CIP 81.96) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.96 |