Strain identifier

BacDive ID: 4356

Type strain: Yes

Species: Edwardsiella ictaluri

Strain Designation: GA 77-52, 1976-78

Strain history: CIP <- 1981, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 1976-78 <- Auburn Univ., USA: strain GA 77-52

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5120

BacDive-ID: 4356

DSM-Number: 13697

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Edwardsiella ictaluri GA 77-52 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that was isolated from channel catfish, Ictalurus punctatus.

NCBI tax id

NCBI tax idMatching level
1027360strain
67780species

strain history

@refhistory
5120<- ATCC <- J. P. Hawke; GA 77-52
67770R. Sakazaki 146 <-- ATCC 33202 <-- J. P. Hawke GA77-52.
120041CIP <- 1981, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 1976-78 <- Auburn Univ., USA: strain GA 77-52

doi: 10.13145/bacdive4356.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Hafniaceae
  • genus: Edwardsiella
  • species: Edwardsiella ictaluri
  • full scientific name: Edwardsiella ictaluri Hawke et al. 1981

@ref: 5120

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Hafniaceae

genus: Edwardsiella

species: Edwardsiella ictaluri

full scientific name: Edwardsiella ictaluri Hawke et al. 1981

strain designation: GA 77-52, 1976-78

type strain: yes

Morphology

cell morphology

  • @ref: 120041
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5120CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5120TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
37629MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120041CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
120041CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5120positivegrowth28mesophilic
37629positivegrowth30mesophilic
67770positivegrowth37mesophilic
120041positivegrowth25-37mesophilic
120041nogrowth15psychrophilic
120041nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120041
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol+builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine+degradation25094
68368arginine-hydrolysis29016
120041nitrate+reduction17632
120041nitrite-reduction16301
120041nitrate+respiration17632

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12004135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-
12004115688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
120041oxidase-
120041alcohol dehydrogenase-1.1.1.1
120041catalase+1.11.1.6
120041lysine decarboxylase+4.1.1.18
120041ornithine decarboxylase-4.1.1.17
120041urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120041-+++-+--+-++-----+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
5120--+--------+---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120041+/---+/-++/----++++--+-+---+-----+---+-----------+/---+-+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
5120channel catfish, Ictalurus punctatusIctalurus punctatusGeorgiaUSAUSANorth America
46881Channel catfish
67770Channel catfish (Ictalurus punctatus)Ictalurus punctatus
120041Animal, Catfish, enteric septicemiaGeorgiaUnited States of AmericaUSANorth America1976

isolation source categories

Cat1Cat2
#Environmental#Aquatic
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_47.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_38;97_40;98_44;99_47&stattab=map
  • Last taxonomy: Edwardsiella
  • 16S sequence: AB050826
  • Sequence Identity:
  • Total samples: 22716
  • soil counts: 509
  • aquatic counts: 2305
  • animal counts: 19360
  • plant counts: 542

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
5120yesyes2Risk group (German classification)
1200411Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Edwardsiella ictaluri ATCC 33202 16S ribosomal RNA gene, partial sequence; short 16S-23S intergenic spacer and tRNA-Glu gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY706738673ena1027360
20218Edwardsiella ictaluri gene for 16S rRNA, partial sequenceAB0508261466ena1027360

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Edwardsiella ictaluri ATCC 332021027360.4wgspatric1027360
66792Edwardsiella ictaluri strain NCTC1212267780.22wgspatric67780
66792Edwardsiella ictaluri ATCC 332022547132403draftimg1027360
66792Edwardsiella ictaluri NCTC 121222850123092draftimg67780
67770Edwardsiella ictaluri ATCC 33202GCA_000264785contigncbi1027360
67770Edwardsiella ictaluri NCTC12122GCA_900447225contigncbi67780

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.295no
flagellatedyes56.267no
gram-positiveno98.673no
anaerobicno79.822no
aerobicyes51.558no
halophileno90.57no
spore-formingno94.901no
thermophileno97.549yes
glucose-utilyes93.23no
glucose-fermentyes92.3yes

External links

@ref: 5120

culture collection no.: CCUG 18764, CECT 885, DSM 13697, ATCC 33202, CDC 1976-78, JCM 16934, JCM 1680, CGMCC 1.1873, CIP 81.96, KCTC 12264, LMG 7860, NBRC 105724, NCIMB 13272, NCTC 12122

straininfo link

  • @ref: 73847
  • straininfo: 389450

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Cultivation1689127Outer membrane protein profiles of Edwardsiella ictaluri from fish.Newton JC, Blevins WT, Wilt GR, Wolfe LGAm J Vet Res1990Animals, Bacterial Outer Membrane Proteins/*analysis, Bacteriological Techniques/veterinary, Catfishes/*microbiology, Culture Media, Electrophoresis, Polyacrylamide Gel, Enterobacteriaceae/*analysis/growth & development, Fishes/microbiology, Ictaluridae/*microbiology, Molecular Weight, Sarcosine/analogs & derivatives, Time FactorsEnzymology
Enzymology3247906Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri.Newton JC, Bird RC, Blevins WT, Wilt GR, Wolfe LGAm J Vet Res1988Animals, Cloning, Molecular, DNA, Bacterial/analysis/genetics/*isolation & purification, Electrophoresis, Agar Gel, Enterobacteriaceae/*genetics/pathogenicity, Fishes, Ictaluridae, *Plasmids, Restriction Mapping, VirulencePhylogeny
Enzymology22017642Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture.Li X, Wu H, Zhang M, Liang S, Xiao J, Wang Q, Liu Q, Zhang YLett Appl Microbiol2011*Aeromonas hydrophila, Animals, Antigens, Bacterial/*immunology, Aquaculture, Bacterial Infections/immunology/prevention & control/veterinary, Bacterial Vaccines/*immunology, *Edwardsiella, Fish Diseases/immunology/prevention & control, Glyceraldehyde-3-Phosphate Dehydrogenases/*immunology, Vaccines, Synthetic/immunology, *Vibrio, Zebrafish10.1111/j.1472-765X.2011.03164.x
Genetics24062549Comparison of Vietnamese and US isolates of Edwardsiella ictaluri.Rogge ML, Dubytska L, Jung TS, Wiles J, Elkamel AA, Rennhoff A, Oanh DT, Thune RLDis Aquat Organ2013Animals, Anti-Bacterial Agents/pharmacology, Aquaculture, DNA Fingerprinting, Drug Resistance, Bacterial, Edwardsiella ictaluri/drug effects/*genetics/pathogenicity, Enterobacteriaceae Infections/epidemiology/microbiology/*veterinary, Fish Diseases/epidemiology/*microbiology, Gene Expression Regulation, Bacterial, Genome, Bacterial, *Ictaluridae, Plasmids/genetics, Specific Pathogen-Free Organisms, United States, Vietnam/epidemiology, VirulencePathogenicity10.3354/dao02620
24293714Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151.Yang Q, Han Y, Tinh NT, Hien NT, Bossier PIndian J Microbiol201210.1007/s12088-012-0312-9

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5120Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13697)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13697
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37629Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11263
46881Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18764)https://www.ccug.se/strain?id=18764
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73847Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389450.1StrainInfo: A central database for resolving microbial strain identifiers
120041Curators of the CIPCollection of Institut Pasteur (CIP 81.96)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.96