Strain identifier

BacDive ID: 4355

Type strain: Yes

Species: Edwardsiella tarda

Strain Designation: K 349, K349, 4-78, 1483-59

Strain history: CIP <- 1978, C. Richard, Inst. Pasteur, Paris, France: strain 4-78 <- NCTC <- CDC: strain 1483-59 <- Kentucky State Hlth. Dept., USA: strain K349

NCBI tax ID(s): 667121 (strain), 636 (species)

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General

@ref: 9101

BacDive-ID: 4355

DSM-Number: 30052

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile, human pathogen

description: Edwardsiella tarda K 349 is a mesophilic, motile human pathogen that was isolated from human faeces.

NCBI tax id

NCBI tax idMatching level
667121strain
636species

strain history

@refhistory
9101<- ATCC <- NCDC, 1483-59 (W.H. Ewing) <- Kentucky State Health Dept., K 349
67770Y. Kosako 82051 <-- R. Sakazaki 123 <-- ATCC 15947 <-- CDC 1483-59 <-- Kentucky State Health Dept., USA.
123429CIP <- 1978, C. Richard, Inst. Pasteur, Paris, France: strain 4-78 <- NCTC <- CDC: strain 1483-59 <- Kentucky State Hlth. Dept., USA: strain K349

doi: 10.13145/bacdive4355.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Hafniaceae
  • genus: Edwardsiella
  • species: Edwardsiella tarda
  • full scientific name: Edwardsiella tarda Ewing and McWhorter 1965 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Paracolobactrum anguillimortiferum
    20215Edwardsiella anguillimortifera

@ref: 9101

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Hafniaceae

genus: Edwardsiella

species: Edwardsiella tarda

full scientific name: Edwardsiella tarda Ewing and McWhorter 1965

strain designation: K 349, K349, 4-78, 1483-59

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes97.17
6948099.95negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9101NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33522MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123429CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
123429CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
9101positivegrowth30mesophilic
33522positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.362

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
6837418257ornithine+degradation
6837429016arginine-hydrolysis
6837425094lysine+degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose+builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose-builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837435581indole+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase-3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase-3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase+4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
9101--++++--+--+---------
9101--++-+--+--+---------

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
44397+-+--------+----+--+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9101human faeces
44397Human fecesKentuckyUSAUSANorth America
67770Human feces
123429Human, FecesKentuckyUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_47.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_38;97_40;98_44;99_47&stattab=map
  • Last taxonomy: Edwardsiella
  • 16S sequence: JX866952
  • Sequence Identity:
  • Total samples: 22716
  • soil counts: 509
  • aquatic counts: 2305
  • animal counts: 19360
  • plant counts: 542

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
9101yesyes2Risk group (German classification)
1234292Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Edwardsiella tarda gene for 16S rRNA, partial sequence, strain:ATCC15947AB0508271399ena667121
20218Edwardsiella tarda ATCC 15947 16S ribosomal RNA gene, partial sequenceJX8669521294ena667121

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Edwardsiella tarda ATCC 15947667121.5wgspatric667121
66792Edwardsiella tarda ATCC 15947 = NBRC 105688667121.11wgspatric667121
66792Edwardsiella tarda NBRC 1056881216965.3wgspatric667121
66792Edwardsiella tarda strain FDAARGOS_1473636.42completepatric636
66792Edwardsiella tarda strain NCTC10396636.30wgspatric636
66792Edwardsiella tarda ATCC 159472547132207draftimg667121
66792Edwardsiella tarda NBRC 1056882563367136draftimg667121
67770Edwardsiella tarda ATCC 15947 = NBRC 105688GCA_000264805contigncbi667121
67770Edwardsiella tarda ATCC 15947 = NBRC 105688GCA_000341505contigncbi667121
67770Edwardsiella tarda ATCC 15947 = NBRC 105688GCA_003113495completencbi667121
67770Edwardsiella tarda NCTC10396GCA_900447455contigncbi636
66792Edwardsiella tarda strain FDAARGOS_1473636.50completepatric636
66792Edwardsiella tarda strain FDAARGOS_1473636.52completepatric636
66792Edwardsiella tarda strain FDAARGOS_1473636.48completepatric636

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.507no
anaerobicno73.176no
halophileno87.067no
spore-formingno94.661no
glucose-utilyes92.052no
thermophileno97.573yes
flagellatedyes54.983no
aerobicno62.039no
motileyes89.701no
glucose-fermentyes91.015yes

External links

@ref: 9101

culture collection no.: DSM 30052, NBRC 105688, ATCC 15947, ATCC 23656, CCM 2238, NCTC 10396, CCUG 1638, LMG 2793, CIP 78.61, JCM 1656, NCDC 1483-59, BCRC 10670, CDC 1483-59, CECT 849

straininfo link

  • @ref: 73846
  • straininfo: 389406

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology10956593Rapid identification of Enterobacteriaceae using a novel 23S rRNA-targeted oligonucleotide probe.Bohnert J, Hubner B, Botzenhart KInt J Hyg Environ Health10.1078/S1438-4639(04)70011-52000Enterobacteriaceae/*genetics/*isolation & purification, *Environmental Monitoring/methods, Humans, *In Situ Hybridization, Fluorescence, Oligonucleotide Probes, Predictive Value of Tests, RNA, Ribosomal, 23S/*isolation & purification, Sequence Analysis, *Water MicrobiologyPhylogeny
Enzymology12552814[Analys of outer membrane protein and drug-resistant of Edwardsiella tarda].Huang X, Lu CWei Sheng Wu Xue Bao2001Anti-Bacterial Agents/*pharmacology, Bacterial Outer Membrane Proteins/*analysis/classification/isolation & purification, Chloramphenicol/pharmacology, *Drug Resistance, Bacterial, Edwardsiella tarda/*chemistry/drug effects, Electrophoresis, Polyacrylamide Gel/methods, Gentamicins/pharmacology, Tetracycline/pharmacologyPathogenicity
20109592Analysis of the vaccine potential of a natural avirulent Edwardsiella tarda isolate.Cheng S, Hu YH, Zhang M, Sun LVaccine10.1016/j.vaccine.2010.01.0232010Administration, Oral, Animals, Antibodies, Bacterial/blood, Bacterial Vaccines/administration & dosage/*immunology, Blood Bactericidal Activity, Edwardsiella tarda/*immunology/pathogenicity, Enterobacteriaceae Infections/pathology/prevention & control/*veterinary, Fish Diseases/microbiology/pathology/*prevention & control, Flounder, Injections, Intraperitoneal, Intestines/microbiology, Liver/microbiology, Spleen/microbiology, Survival Analysis, Vaccines, Attenuated/administration & dosage/immunology, Virulence
21514342Construction and evaluation of a live vaccine against Edwardsiella tarda and Vibrio harveyi: laboratory vs. mock field trial.Hu YH, Cheng S, Zhang M, Sun LVaccine10.1016/j.vaccine.2011.04.0252011Administration, Oral, Animals, Antibodies, Bacterial/blood, Bacterial Vaccines/administration & dosage/*immunology, Edwardsiella tarda/*immunology, Enterobacteriaceae Infections/immunology/prevention & control/*veterinary, Fish Diseases/immunology/*prevention & control, Flatfishes, Injections, Intraperitoneal, Survival Analysis, Vaccines, Synthetic/administration & dosage/immunology, Vibrio/*immunology, Vibrio Infections/immunology/prevention & control/*veterinary
Phylogeny21679202Phenotypic diversity of Edwardsiella tarda isolated from different origins.He Y, Xu T, Han Y, Shi X, Zhang XHLett Appl Microbiol10.1111/j.1472-765X.2011.03103.x2011Animals, Edwardsiella tarda/*classification/*genetics/isolation & purification/physiology, Enterobacteriaceae Infections/*microbiology/*veterinary, *Fishes, Flagella/*physiology, Humans, Phenotype, PlasmidsPathogenicity
Stress30201590Stable isotope labelling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Edwardsiella tarda ATCC 15947 under prolonged cold stress.Ma W, Jia J, Huang X, Xie W, Zhang X, Tang J, Lin C, Zhao L, Fang PMicrob Pathog10.1016/j.micpath.2018.09.0062018Amino Acids/*metabolism, Cell Culture Techniques/methods, *Cold-Shock Response, Edwardsiella tarda/*physiology/*radiation effects, Isotope Labeling/*methods, Proteomics/*methodsMetabolism
Enzymology33375969Cloning and characterization of two chondroitin sulfate ABC lyases from Edwardsiella tarda LMG2793.Song G, Sun J, Zhao M, Wang Z, Gong Q, Yu WEnzyme Microb Technol10.1016/j.enzmictec.2020.1097012020*Chondroitin ABC Lyase/genetics, *Chondroitin Sulfates, Cloning, Molecular, Edwardsiella tarda/genetics, Ions
Pathogenicity35916068Phenotypic and genotypic analysis of Edwardsiella isolates from Taiwan indicates wide variation with a particular reference to Edwardsiella tarda and Edwardsiella anguillarum.Rahmawaty A, Chen MY, Byadgi OV, Wang PC, Chen SCJ Fish Dis10.1111/jfd.136882022Animals, *Edwardsiella/genetics, Edwardsiella tarda/genetics, *Enterobacteriaceae Infections/microbiology/veterinary, *Fish Diseases/microbiology, Fishes/microbiology, Humans, Phenotype, Phylogeny, TaiwanPhenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9101Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30052)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30052
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33522Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11011
44397Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 1638)https://www.ccug.se/strain?id=1638
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
73846Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389406.1StrainInfo: A central database for resolving microbial strain identifiers
123429Curators of the CIPCollection of Institut Pasteur (CIP 78.61)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2078.61