Strain identifier

BacDive ID: 4345

Type strain: Yes

Species: Citrobacter sedlakii

Strain Designation: I-25, I-75, 4696-86

Strain history: CIP <- 1996, P.A.D. Grimont, Inst. Pasteur, Paris, France: strain I-75 <- CDC: strain 4696-86

NCBI tax ID(s): 67826 (species)

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General

@ref: 7116

BacDive-ID: 4345

DSM-Number: 17674

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Citrobacter sedlakii I-25 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from human faeces.

NCBI tax id

  • NCBI tax id: 67826
  • Matching level: species

strain history

@refhistory
7116<- CIP <- P. A. D. Grimont, IP; I-25
122539CIP <- 1996, P.A.D. Grimont, Inst. Pasteur, Paris, France: strain I-75 <- CDC: strain 4696-86

doi: 10.13145/bacdive4345.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Citrobacter
  • species: Citrobacter sedlakii
  • full scientific name: Citrobacter sedlakii Brenner et al. 1993

@ref: 7116

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Citrobacter

species: Citrobacter sedlakii

full scientific name: Citrobacter sedlakii Brenner et al. 1993

strain designation: I-25, I-75, 4696-86

type strain: yes

Morphology

cell morphology

  • @ref: 122539
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7116COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7116TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
39171MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122539CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
122539CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
7116positivegrowth30
39171positivegrowth30
50274positivegrowth37
122539positivegrowth5-41

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7116aerobe
122539facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12253929864mannitol+fermentation
12253916947citrate+carbon source
12253917234glucose+fermentation
12253917716lactose-fermentation
12253917632nitrate+reduction
12253916301nitrite-reduction
12253915792malonate+assimilation
122539132112sodium thiosulfate-builds gas from
12253917234glucose+degradation
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
68371Potassium 2-ketogluconate+builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose+builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
683714853esculin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol+builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose+builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

antibiotic resistance

  • @ref: 122539
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
12253935581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole test
12253915688acetoin-
12253917234glucose+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122539oxidase-
122539beta-galactosidase+3.2.1.23
122539alcohol dehydrogenase-1.1.1.1
122539gelatinase-
122539catalase+1.11.1.6
122539lysine decarboxylase-4.1.1.18
122539ornithine decarboxylase+4.1.1.17
122539phenylalanine ammonia-lyase-4.3.1.24
122539tryptophan deaminase-
122539urease-3.5.1.5
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122539-+++-+----++-+------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7116++-++---+--+++/-++-+++-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122539+-++++---++++/--+++++--+-+/--+/-++++-+------+/-----+--++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122539+++++-+--+++++++-+-+++-++----+-++---+-----+++--++++++++++--++-++-+++-+---+-+---+++++--+-+++++++++-+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7116human faecesFranceFRAEurope
50274Human feces
122539Human, FecesFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71162Risk group (German classification)
1225391Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7116
  • description: Citrobacter sedlakii 16S ribosomal RNA gene, partial sequence
  • accession: AF025364
  • length: 1522
  • database: nuccore
  • NCBI tax ID: 67826

External links

@ref: 7116

culture collection no.: DSM 17674, ATCC 51115, CDC 4696-86, CIP 105037, CCUG 30794

straininfo link

  • @ref: 73836
  • straininfo: 42811

Reference

@idauthorscataloguedoi/urltitle
7116Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17674)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17674
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39171Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17012
50274Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30794)https://www.ccug.se/strain?id=30794
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
73836Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42811.1StrainInfo: A central database for resolving microbial strain identifiers
122539Curators of the CIPCollection of Institut Pasteur (CIP 105037)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105037