Strain identifier

BacDive ID: 4337

Type strain: Yes

Species: Citrobacter murliniae

Strain Designation: C5775, 2970-59

Strain history: CIP <- 1995, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 2970-59

NCBI tax ID(s): 67829 (species)

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General

@ref: 5119

BacDive-ID: 4337

DSM-Number: 13695

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Citrobacter murliniae C5775 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 67829
  • Matching level: species

strain history

@refhistory
5119<- ATCC <- C. F. Riddle <- Illinois State Health Dept.
121200CIP <- 1995, P.A.D. Grimont, Inst. Pasteur, Paris, France <- CDC: strain 2970-59

doi: 10.13145/bacdive4337.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Citrobacter
  • species: Citrobacter murliniae
  • full scientific name: Citrobacter murliniae Brenner et al. 2000

@ref: 5119

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Citrobacter

species: Citrobacter murliniae

full scientific name: Citrobacter murliniae Brenner et al. 2000

strain designation: C5775, 2970-59

type strain: yes

Morphology

cell morphology

  • @ref: 121200
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5119NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35691MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
121200CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
121200CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
5119positivegrowth37mesophilic
35691positivegrowth30mesophilic
50277positivegrowth37mesophilic
121200positivegrowth15-41
121200nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121200
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371esculin-builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol+builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose+builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
121200citrate+carbon source16947
121200glucose+fermentation17234
121200lactose-fermentation17716
121200nitrate+reduction17632
121200nitrite-reduction16301
121200malonate-assimilation15792
121200sodium thiosulfate+builds gas from132112
121200glucose+degradation17234

antibiotic resistance

  • @ref: 121200
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121200
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12120015688acetoin-
12120017234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121200oxidase-
121200beta-galactosidase+3.2.1.23
121200alcohol dehydrogenase-1.1.1.1
121200gelatinase-
121200catalase-1.11.1.6
121200lysine decarboxylase-4.1.1.18
121200ornithine decarboxylase-4.1.1.17
121200urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121200-++--+++--++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121200+-++++---+++++++-++--+-+-+/-+++/---+---+/---+/--+/---+--+++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
5119IllinoisUSAUSANorth America
50277IllinoisUSAUSANorth AmericaSource not known

taxonmaps

  • @ref: 69479
  • File name: preview.99_644.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_644&stattab=map
  • Last taxonomy: Citrobacter
  • 16S sequence: AF025369
  • Sequence Identity:
  • Total samples: 66090
  • soil counts: 3252
  • aquatic counts: 7413
  • animal counts: 52478
  • plant counts: 2947

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51192Risk group (German classification)
1212001Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Citrobacter sp. 'genomospecies 11' 16S ribosomal RNA gene, partial sequence
  • accession: AF025369
  • length: 1530
  • database: ena
  • NCBI tax ID: 67829

External links

@ref: 5119

culture collection no.: CIP 104556, CCM 4834, DSM 13695, ATCC 51118, CCUG 30797, CDC 2970-59

straininfo link

  • @ref: 73829
  • straininfo: 93027

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny34037857Generation of electrical energy in a microbial fuel cell coupling acetate oxidation to Fe(3+) reduction and isolation of the involved bacteria.Becerril-Varela K, Serment-Guerrero JH, Manzanares-Leal GL, Ramirez-Duran N, Guerrero-Barajas CWorld J Microbiol Biotechnol10.1007/s11274-021-03077-42021Acetates/metabolism, Bacteria/*classification/genetics/isolation & purification, Biodegradation, Environmental, Bioelectric Energy Sources/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Iron/*chemistry, Oxidation-Reduction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA/*methods, Sewage/microbiologyTranscriptome
36121562Iron reducing sludge as a source of electroactive bacteria: assessing iron reduction in biofilm bacteria, planktonic cells and isolates from a microbial fuel cell.Gonzalez-Paz JR, Becerril-Varela K, Guerrero-Barajas CArch Microbiol10.1007/s00203-022-03253-62022*Bioelectric Energy Sources/microbiology, Biofilms, Carbon, Electricity, Iron, Plankton, Sewage

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5119Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13695)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13695
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35691Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16477
50277Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30797)https://www.ccug.se/strain?id=30797
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73829Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID93027.1StrainInfo: A central database for resolving microbial strain identifiers
121200Curators of the CIPCollection of Institut Pasteur (CIP 104556)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104556