Strain identifier
BacDive ID: 433
Type strain:
Species: Alicyclobacillus shizuokensis
Strain Designation: 4A-336
Strain history: IAM 15226 <-- K. Goto 4-A336.
NCBI tax ID(s): 1220573 (strain), 392014 (species)
General
@ref: 7313
BacDive-ID: 433
DSM-Number: 17981
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Alicyclobacillus shizuokensis 4A-336 is an aerobe, spore-forming, thermophilic bacterium that was isolated from soil, crop field.
NCBI tax id
NCBI tax id | Matching level |
---|---|
392014 | species |
1220573 | strain |
strain history
@ref | history |
---|---|
7313 | <- Keiichi Goto, 4-A336 |
67770 | IAM 15226 <-- K. Goto 4-A336. |
doi: 10.13145/bacdive433.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Alicyclobacillaceae
- genus: Alicyclobacillus
- species: Alicyclobacillus shizuokensis
- full scientific name: Alicyclobacillus shizuokensis Goto et al. 2007
@ref: 7313
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Alicyclobacillaceae
genus: Alicyclobacillus
species: Alicyclobacillus shizuokensis
full scientific name: Alicyclobacillus shizuokensis Goto et al. 2007
strain designation: 4A-336
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
31943 | positive | 04-05 µm | 0.7-0.8 µm | rod-shaped | yes | |
69480 | positive | 100 |
pigmentation
- @ref: 31943
- production: no
Culture and growth conditions
culture medium
- @ref: 7313
- name: BACILLUS ACIDOCALDARIUS MEDIUM (DSMZ Medium 13)
- growth: yes
- link: https://mediadive.dsmz.de/medium/13
- composition: Name: BACILLUS ACIDOCALDARIUS MEDIUM (DSMZ Medium 13) Composition: Agar 20.0 g/l Glucose 1.0 g/l Yeast extract 1.0 g/l KH2PO4 0.6 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
7313 | positive | growth | 50 | thermophilic | |
31943 | positive | growth | 35-60 | ||
31943 | positive | optimum | 45-50 | thermophilic | |
67770 | positive | growth | 50 | thermophilic | |
69480 | thermophilic | 99.999 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31943 | positive | growth | 4.0-4.5 | acidophile |
31943 | positive | optimum | 4.25 |
Physiology and metabolism
oxygen tolerance
- @ref: 31943
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
31943 | yes | |
69481 | yes | 95 |
69480 | yes | 95.405 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31943 | NaCl | positive | growth | 0-5 % |
31943 | NaCl | positive | optimum | 2.5 % |
observation
- @ref: 67770
- observation: quinones: MK-7
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31943 | 22599 | arabinose | + | carbon source |
31943 | 17057 | cellobiose | + | carbon source |
31943 | 28260 | galactose | + | carbon source |
31943 | 17306 | maltose | + | carbon source |
31943 | 29864 | mannitol | + | carbon source |
31943 | 28053 | melibiose | + | carbon source |
31943 | 17814 | salicin | + | carbon source |
31943 | 17992 | sucrose | + | carbon source |
31943 | 27082 | trehalose | + | carbon source |
31943 | 18222 | xylose | + | carbon source |
enzymes
- @ref: 31943
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
7313 | soil, crop field | Shizuoka city | Japan | JPN | Asia |
67770 | Soil of a crop field in Shizuoka City | Shizuoka Pref. | Japan | JPN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Field |
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
taxonmaps
- @ref: 69479
- File name: preview.99_5562.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_313;96_2685;97_3297;98_4150;99_5562&stattab=map
- Last taxonomy: Alicyclobacillus shizuokensis
- 16S sequence: AB264024
- Sequence Identity:
- Total samples: 348
- soil counts: 122
- aquatic counts: 17
- animal counts: 176
- plant counts: 33
Safety information
risk assessment
- @ref: 7313
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7313
- description: Alicyclobacillus shizuokensis gene for 16S ribosomal RNA, partial sequence
- accession: AB264024
- length: 1519
- database: ena
- NCBI tax ID: 392014
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Alicyclobacillus shizuokensis NBRC 103103 | 1220573.3 | wgs | patric | 1220573 |
66792 | Alicyclobacillus shizuokensis NBRC 103103 | 2731957577 | draft | img | 1220573 |
67770 | Alicyclobacillus shizuokensis NBRC 103103 | GCA_001552255 | contig | ncbi | 1220573 |
GC content
@ref | GC-content | method |
---|---|---|
7313 | 60.5 | |
67770 | 60.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 95 | no |
motile | yes | 86.064 | yes |
gram-positive | yes | 83.563 | no |
anaerobic | no | 99.047 | yes |
aerobic | yes | 88.608 | yes |
halophile | no | 89.982 | no |
spore-forming | yes | 95.874 | yes |
glucose-util | yes | 90.331 | no |
flagellated | no | 67.997 | no |
thermophile | yes | 99.998 | yes |
glucose-ferment | no | 91.843 | no |
External links
@ref: 7313
culture collection no.: DSM 17981, IAM 15226, JCM 21680, NBRC 103103
straininfo link
- @ref: 70112
- straininfo: 309423
literature
- topic: Phylogeny
- Pubmed-ID: 17551043
- title: Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov.
- authors: Goto K, Mochida K, Kato Y, Asahara M, Fujita R, An SY, Kasai H, Yokota A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.64692-0
- year: 2007
- mesh: Aerobiosis, Bacterial Proteins/genetics, Bacterial Typing Techniques, Beverages/*microbiology, Carbohydrate Metabolism, Carboxylic Acids/metabolism, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Genes, rRNA, Gram-Positive Endospore-Forming Bacteria/*classification/cytology/*isolation & purification/physiology, Guaiacol/metabolism, Hydrogen-Ion Concentration, Microscopy, Phase-Contrast, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Temperature
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7313 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17981) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17981 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31943 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28199 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
70112 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID309423.1 | StrainInfo: A central database for resolving microbial strain identifiers |