Strain identifier

BacDive ID: 4309

Type strain: Yes

Species: Brenneria alni

Strain history: CIP <- 1996, G. Surico, Florence Univ., Florence, Italy: strain PVFi 20

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General

@ref: 4498

BacDive-ID: 4309

DSM-Number: 11811

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, plant pathogen

description: Brenneria alni CCUG 48887 is an aerobe, mesophilic, Gram-negative plant pathogen that was isolated from bark cancer of alder.

NCBI tax id

NCBI tax idMatching level
71656species
1121119strain

strain history

@refhistory
4498<- NCPPB <- G. Surico, Univ. of Florence; PVFi20
391381996, G. Surico, Inst. Pato. Veg., Firenze, Italy: strain PVFi 20
116262CIP <- 1996, G. Surico, Florence Univ., Florence, Italy: strain PVFi 20

doi: 10.13145/bacdive4309.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Pectobacteriaceae
  • genus: Brenneria
  • species: Brenneria alni
  • full scientific name: Brenneria alni (Surico et al. 1996) Hauben et al. 1999
  • synonyms

    • @ref: 20215
    • synonym: Erwinia alni

@ref: 4498

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Pectobacteriaceae

genus: Brenneria

species: Brenneria alni

full scientific name: Brenneria alni (Surico et al. 1996) Hauben et al. 1999

type strain: yes

Morphology

cell morphology

  • @ref: 116262
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4498NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39138MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116262CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4498positivegrowth30mesophilic
39138positivegrowth30mesophilic
58383positivegrowth22-37
116262positivegrowth30-37mesophilic
116262nogrowth5psychrophilic
116262nogrowth10psychrophilic
116262nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58383aerobe
116262facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose+builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose-fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
116262mannitol+fermentation29864
116262citrate-carbon source16947
116262glucose+fermentation17234
116262lactose+fermentation17716
116262nitrate-reduction17632
116262nitrite-reduction16301
116262malonate-assimilation15792
116262sodium thiosulfate-builds gas from132112
116262glucose+degradation17234

antibiotic resistance

  • @ref: 116262
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11626235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11626215688acetoin+
11626217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116262oxidase-
116262beta-galactosidase+3.2.1.23
116262alcohol dehydrogenase-1.1.1.1
116262gelatinase-
116262catalase+1.11.1.6
116262lysine decarboxylase-4.1.1.18
116262ornithine decarboxylase-4.1.1.17
116262phenylalanine ammonia-lyase-4.3.1.24
116262tryptophan deaminase-
116262urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116262-+++-++-+-++---+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
4498-----------++---+-++-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116262+-++++---++++----+--+++++++/-+--++/--------+---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116262+++++--+-++------++++++--------+++-++----+-------+------+--++------------------++--------+----+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
4498bark cancer of alderTuscanyItalyITAEurope
58383Bark canker on stem of Italian alder,Alnus cordataTuscanyItalyITAEurope1990
116262Bark canker on Alnus cordata stemTuscanyItalyITAEurope1990

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Bark

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
4498yes1Risk group (German classification)
1162621Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Erwinia alni 16S rRNA gene (strain DSM 11811)AJ2334091496ena71656
20218Brenneria alni ex Erwinia alni 16S rRNA geneAJ2234681502ena71656
20218Brenneria alni strain pvfi20 16S ribosomal RNA gene, partial sequenceEU4906031224ena71656

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brenneria alni NCPPB 3934GCA_003666245contigncbi71656
66792Brenneria alni strain NCPPB 393471656.4wgspatric71656
66792Brenneria alni NCPPB 39342835052945draftimg71656
66792Brenneria alni DSM 118112574179710draftimg1121119

External links

@ref: 4498

culture collection no.: CCUG 48887, ICMP 12481, ATCC 700181, CIP 104916, DSM 11811, NCPPB 3934, PVFi 20

straininfo link

  • @ref: 73801
  • straininfo: 43812

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4498Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11811)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11811
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39138Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16877
58383Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48887)https://www.ccug.se/strain?id=48887
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
73801Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43812.1StrainInfo: A central database for resolving microbial strain identifiers
116262Curators of the CIPCollection of Institut Pasteur (CIP 104916)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104916