Strain identifier
BacDive ID: 43
Type strain:
Species: Gluconacetobacter azotocaptans
Strain Designation: CFN-Ca54, CFN-Ca54T
Strain history: CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico, strain: CFN-Ca54T <- T. Jimenez-Salgado
NCBI tax ID(s): 1307932 (strain), 142834 (species)
General
@ref: 5102
BacDive-ID: 43
DSM-Number: 13594
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Gluconacetobacter azotocaptans CFN-Ca54 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of coffee plants.
NCBI tax id
NCBI tax id | Matching level |
---|---|
142834 | species |
1307932 | strain |
strain history
@ref | history |
---|---|
5102 | <- J. Caballero-Mellado; CFN-Ca54 <- T. Jiménez-Salgado |
116690 | CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico, strain: CFN-Ca54T <- T. Jimenez-Salgado |
doi: 10.13145/bacdive43.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Gluconacetobacter
- species: Gluconacetobacter azotocaptans
- full scientific name: Gluconacetobacter azotocaptans Fuentes-Ramírez et al. 2001
@ref: 5102
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Gluconacetobacter
species: Gluconacetobacter azotocaptans
full scientific name: Gluconacetobacter azotocaptans Fuentes-Ramírez et al. 2001
strain designation: CFN-Ca54, CFN-Ca54T
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement |
---|---|---|---|---|---|---|
22919 | negative | 1.6-2.0 µm | 0.5-0.6 µm | rod-shaped | yes | peritrichous |
116690 | negative | rod-shaped | no |
colony morphology
- @ref: 22919
- colony color: beige to light-brownish
- medium used: potato agar
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5102 | GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920) | yes | https://mediadive.dsmz.de/medium/920 | Name: GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920) Composition: Agar 18.0 g/l K2HPO4 4.81 g/l MES buffer 4.4 g/l Glucose 2.7 g/l Yeast extract 2.7 g/l Mannitol 1.8 g/l KH2PO4 0.65 g/l Distilled water |
22919 | LGI | yes | ||
22919 | potato agar | yes | ||
33648 | MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g) | |
116690 | CIP Medium 1 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5102 | positive | growth | 28 | mesophilic |
22919 | positive | growth | 29 | mesophilic |
22919 | no | growth | 37 | mesophilic |
33648 | positive | growth | 30 | mesophilic |
116690 | positive | growth | 22-37 | |
116690 | no | growth | 10 | psychrophilic |
116690 | no | growth | 41 | thermophilic |
116690 | no | growth | 45 | thermophilic |
culture pH
- @ref: 22919
- ability: positive
- type: growth
- pH: 4-7
- PH range: acidophile
Physiology and metabolism
tolerance
@ref | compound | percentage |
---|---|---|
22919 | Glucose | 30 |
22919 | Sucrose | 30 |
oxygen tolerance
@ref | oxygen tolerance |
---|---|
22919 | aerobe |
116690 | obligate aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
22919 | NaCl | positive | growth | 0.25-0.5 % |
22919 | NaCl | no | growth | 1 % |
116690 | NaCl | positive | growth | 0 % |
116690 | NaCl | no | growth | 2 % |
116690 | NaCl | no | growth | 4 % |
116690 | NaCl | no | growth | 6 % |
116690 | NaCl | no | growth | 8 % |
116690 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22919 | 28885 | butanol | - | growth |
22919 | 17108 | D-arabinose | - | growth |
22919 | 16899 | D-mannitol | - | growth |
22919 | 16634 | raffinose | - | growth |
22919 | 65327 | D-xylose | - | growth |
22919 | 17754 | glycerol | - | growth |
22919 | 17306 | maltose | - | growth |
22919 | 37684 | mannose | - | growth |
22919 | 28053 | melibiose | - | growth |
22919 | 17790 | methanol | - | growth |
22919 | 17203 | L-proline | - | nitrogen source |
22919 | 16828 | L-tryptophan | - | nitrogen source |
22919 | 17632 | nitrate | - | reduction |
22919 | 17634 | D-glucose | + | builds acid from |
22919 | 28120 | L-fructose | + | builds acid from |
22919 | 17992 | sucrose | + | builds acid from |
22919 | 28938 | ammonium | + | growth |
22919 | 15824 | D-fructose | + | growth |
22919 | 17634 | D-glucose | + | growth |
22919 | 17924 | D-sorbitol | + | growth |
22919 | 16236 | ethanol | + | growth |
22919 | 17992 | sucrose | + | growth |
22919 | 17561 | L-cysteine | + | nitrogen source |
22919 | 29985 | L-glutamate | + | nitrogen source |
22919 | 30089 | acetate | + | oxidation |
22919 | 16236 | ethanol | + | oxidation |
22919 | 24996 | lactate | + | oxidation |
22919 | 12936 | D-galactose | +/- | growth |
22919 | 17924 | D-sorbitol | +/- | growth |
22919 | 16236 | ethanol | +/- | growth |
22919 | 17306 | maltose | +/- | growth |
22919 | 28053 | melibiose | +/- | growth |
116690 | 606565 | hippurate | + | hydrolysis |
116690 | 17632 | nitrate | - | reduction |
116690 | 16301 | nitrite | - | reduction |
antibiotic resistance
- @ref: 116690
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 116690
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
22919 | catalase | + | 1.11.1.6 |
22919 | cytochrome oxidase | - | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
116690 | oxidase | - | |
116690 | beta-galactosidase | - | 3.2.1.23 |
116690 | gelatinase | - | |
116690 | catalase | + | 1.11.1.6 |
116690 | gamma-glutamyltransferase | - | 2.3.2.2 |
116690 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116690 | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116690 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
5102 | rhizosphere of coffee plants | Chiapas | Mexico | MEX | North America | |
116690 | Environment, Coffee plants, rhizosphere | Chiapas | Mexico | MEX | North America | 1996 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
taxonmaps
- @ref: 69479
- File name: preview.99_94670.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_64;97_2865;98_3566;99_94670&stattab=map
- Last taxonomy: Gluconacetobacter
- 16S sequence: JF793973
- Sequence Identity:
- Total samples: 159
- soil counts: 25
- aquatic counts: 18
- animal counts: 66
- plant counts: 50
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5102 | 1 | Risk group (German classification) |
116690 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Acetobacter azotocaptans 16S ribosomal RNA gene, partial sequence | AF192761 | 1447 | ena | 142834 |
20218 | Gluconacetobacter azotocaptans strain DSM 13594 16S ribosomal RNA gene, partial sequence | JF793973 | 1357 | ena | 142834 |
20218 | Gluconacetobacter azotocaptans strain LMG 21311 16S ribosomal RNA gene, partial sequence | JF793972 | 1357 | ena | 142834 |
GC content
- @ref: 22919
- GC-content: 64.01
External links
@ref: 5102
culture collection no.: DSM 13594, ATCC 700988, CIP 107161
straininfo link
- @ref: 69728
- straininfo: 86039
literature
- topic: Phylogeny
- Pubmed-ID: 11491326
- title: Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants.
- authors: Fuentes-Ramirez LE, Bustillos-Cristales R, Tapia-Hernandez A, Jimenez-Salgado T, Wang ET, Martinez-Romero E, Caballero-Mellado J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-51-4-1305
- year: 2001
- mesh: Acetic Acid/metabolism, Acetobacteraceae/*classification/genetics/*isolation & purification/metabolism, Base Sequence, Coffee/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, Mexico, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, Phenotype, Phylogeny, Species Specificity, Terminology as Topic
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5102 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13594) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13594 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
22919 | L E Fuentes-Ramírez, R Bustillos-Cristales, A Tapia-Hernández, T Jiménez-Salgado, E T Wang, E Martínez-Romero, J Caballero-Mellado | 10.1099/00207713-51-4-1305 | Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants. | IJSEM 51: 1305-1314 2001 | 11491326 | |
33648 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/4566 | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69728 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID86039.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116690 | Curators of the CIP | Collection of Institut Pasteur (CIP 107161) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107161 |