Strain identifier

BacDive ID: 43

Type strain: Yes

Species: Gluconacetobacter azotocaptans

Strain Designation: CFN-Ca54, CFN-Ca54T

Strain history: CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico, strain: CFN-Ca54T <- T. Jimenez-Salgado

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General

@ref: 5102

BacDive-ID: 43

DSM-Number: 13594

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Gluconacetobacter azotocaptans CFN-Ca54 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of coffee plants.

NCBI tax id

NCBI tax idMatching level
142834species
1307932strain

strain history

@refhistory
5102<- J. Caballero-Mellado; CFN-Ca54 <- T. Jiménez-Salgado
116690CIP <- 2001, J. Caballero-Mellado, Centro de Investigacion Sobre Fijacion de Nitrogeno, UNAM, Cuernavaca, Morelos, Mexico, strain: CFN-Ca54T <- T. Jimenez-Salgado

doi: 10.13145/bacdive43.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Gluconacetobacter
  • species: Gluconacetobacter azotocaptans
  • full scientific name: Gluconacetobacter azotocaptans Fuentes-Ramírez et al. 2001

@ref: 5102

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Gluconacetobacter

species: Gluconacetobacter azotocaptans

full scientific name: Gluconacetobacter azotocaptans Fuentes-Ramírez et al. 2001

strain designation: CFN-Ca54, CFN-Ca54T

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
22919negative1.6-2.0 µm0.5-0.6 µmrod-shapedyesperitrichous
116690negativerod-shapedno

colony morphology

  • @ref: 22919
  • colony color: beige to light-brownish
  • medium used: potato agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5102GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920)yeshttps://mediadive.dsmz.de/medium/920Name: GLUCONACETOBACTER JOHANNAE AND G. AZOTOCAPTANS MEDIUM (DSMZ Medium 920) Composition: Agar 18.0 g/l K2HPO4 4.81 g/l MES buffer 4.4 g/l Glucose 2.7 g/l Yeast extract 2.7 g/l Mannitol 1.8 g/l KH2PO4 0.65 g/l Distilled water
22919LGIyes
22919potato agaryes
33648MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
116690CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
5102positivegrowth28mesophilic
22919positivegrowth29mesophilic
22919nogrowth37mesophilic
33648positivegrowth30mesophilic
116690positivegrowth22-37
116690nogrowth10psychrophilic
116690nogrowth41thermophilic
116690nogrowth45thermophilic

culture pH

  • @ref: 22919
  • ability: positive
  • type: growth
  • pH: 4-7
  • PH range: acidophile

Physiology and metabolism

tolerance

@refcompoundpercentage
22919Glucose30
22919Sucrose30

oxygen tolerance

@refoxygen tolerance
22919aerobe
116690obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
22919NaClpositivegrowth0.25-0.5 %
22919NaClnogrowth1 %
116690NaClpositivegrowth0 %
116690NaClnogrowth2 %
116690NaClnogrowth4 %
116690NaClnogrowth6 %
116690NaClnogrowth8 %
116690NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2291928885butanol-growth
2291917108D-arabinose-growth
2291916899D-mannitol-growth
2291916634raffinose-growth
2291965327D-xylose-growth
2291917754glycerol-growth
2291917306maltose-growth
2291937684mannose-growth
2291928053melibiose-growth
2291917790methanol-growth
2291917203L-proline-nitrogen source
2291916828L-tryptophan-nitrogen source
2291917632nitrate-reduction
2291917634D-glucose+builds acid from
2291928120L-fructose+builds acid from
2291917992sucrose+builds acid from
2291928938ammonium+growth
2291915824D-fructose+growth
2291917634D-glucose+growth
2291917924D-sorbitol+growth
2291916236ethanol+growth
2291917992sucrose+growth
2291917561L-cysteine+nitrogen source
2291929985L-glutamate+nitrogen source
2291930089acetate+oxidation
2291916236ethanol+oxidation
2291924996lactate+oxidation
2291912936D-galactose+/-growth
2291917924D-sorbitol+/-growth
2291916236ethanol+/-growth
2291917306maltose+/-growth
2291928053melibiose+/-growth
116690606565hippurate+hydrolysis
11669017632nitrate-reduction
11669016301nitrite-reduction

antibiotic resistance

  • @ref: 116690
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116690
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
22919catalase+1.11.1.6
22919cytochrome oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
116690oxidase-
116690beta-galactosidase-3.2.1.23
116690gelatinase-
116690catalase+1.11.1.6
116690gamma-glutamyltransferase-2.3.2.2
116690urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116690-----+--+-----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116690+-----------------------------------------------------------+-+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5102rhizosphere of coffee plantsChiapasMexicoMEXNorth America
116690Environment, Coffee plants, rhizosphereChiapasMexicoMEXNorth America1996

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_94670.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_64;97_2865;98_3566;99_94670&stattab=map
  • Last taxonomy: Gluconacetobacter
  • 16S sequence: JF793973
  • Sequence Identity:
  • Total samples: 159
  • soil counts: 25
  • aquatic counts: 18
  • animal counts: 66
  • plant counts: 50

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51021Risk group (German classification)
1166901Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter azotocaptans 16S ribosomal RNA gene, partial sequenceAF1927611447ena142834
20218Gluconacetobacter azotocaptans strain DSM 13594 16S ribosomal RNA gene, partial sequenceJF7939731357ena142834
20218Gluconacetobacter azotocaptans strain LMG 21311 16S ribosomal RNA gene, partial sequenceJF7939721357ena142834

GC content

  • @ref: 22919
  • GC-content: 64.01

External links

@ref: 5102

culture collection no.: DSM 13594, ATCC 700988, CIP 107161

straininfo link

  • @ref: 69728
  • straininfo: 86039

literature

  • topic: Phylogeny
  • Pubmed-ID: 11491326
  • title: Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants.
  • authors: Fuentes-Ramirez LE, Bustillos-Cristales R, Tapia-Hernandez A, Jimenez-Salgado T, Wang ET, Martinez-Romero E, Caballero-Mellado J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-4-1305
  • year: 2001
  • mesh: Acetic Acid/metabolism, Acetobacteraceae/*classification/genetics/*isolation & purification/metabolism, Base Sequence, Coffee/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Genes, Bacterial, Mexico, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, Phenotype, Phylogeny, Species Specificity, Terminology as Topic
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5102Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13594)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13594
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22919L E Fuentes-Ramírez, R Bustillos-Cristales, A Tapia-Hernández, T Jiménez-Salgado, E T Wang, E Martínez-Romero, J Caballero-Mellado10.1099/00207713-51-4-1305Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants.IJSEM 51: 1305-1314 200111491326
33648Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4566
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69728Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86039.1StrainInfo: A central database for resolving microbial strain identifiers
116690Curators of the CIPCollection of Institut Pasteur (CIP 107161)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107161