Strain identifier

BacDive ID: 428

Type strain: Yes

Species: Alicyclobacillus sacchari

Strain Designation: RB-718

Culture col. no.: DSM 17974, IAM 15230

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7306

BacDive-ID: 428

DSM-Number: 17974

keywords: gram-positive, thermophilic, spore-forming, aerobe, Bacteria, 16S sequence, genome sequence, motile, rod-shaped

description: Alicyclobacillus sacchari RB-718 is an aerobe, spore-forming, thermophilic bacterium that was isolated from liquid sugar.

strain history: <- Keiichi Goto, RB718

doi: 10.13145/bacdive428.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Caryophanales
  • family: Alicyclobacillaceae
  • genus: Alicyclobacillus
  • species: Alicyclobacillus sacchari
  • full scientific name: Alicyclobacillus sacchari Goto et al. 2007

@ref: 7306

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Alicyclobacillaceae

genus: Alicyclobacillus

species: Alicyclobacillus sacchari

full scientific name: Alicyclobacillus sacchari Goto et al. 2007

strain designation: RB-718

type strain: yes

Morphology

cell morphology

  • @ref: 31943
  • gram stain: positive
  • cell length: 04-05 µm
  • cell width: 0.6-0.7 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 31943
  • production: no

Culture and growth conditions

culture medium

  • @ref: 7306
  • name: BACILLUS ACIDOCALDARIUS MEDIUM (DSMZ Medium 13), + MnSO4 (see medium 1), pH:4.2
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium13.pdf

culture temp

@refgrowthtypetemperaturerange
7306positivegrowth50thermophilic
31943positivegrowth30-55
31943positiveoptimum45-50thermophilic

culture pH

@refabilitytypepH
31943positivegrowth4.0-4.5
31943positiveoptimum4.25

Physiology and metabolism

oxygen tolerance

  • @ref: 31943
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31943
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
31943NaClpositivegrowth0-2 %
31943NaClpositiveoptimum1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3194322599arabinose+carbon source
3194317057cellobiose+carbon source
3194328260galactose+carbon source
3194317754glycerol+carbon source
3194328087glycogen+carbon source
3194317716lactose+carbon source
3194317306maltose+carbon source
3194329864mannitol+carbon source
3194328053melibiose+carbon source
3194316634raffinose+carbon source
3194326546rhamnose+carbon source
3194317814salicin+carbon source
3194317992sucrose+carbon source
3194327082trehalose+carbon source
3194318222xylose+carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 7306
  • sample type: liquid sugar
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Sugary food

Safety information

risk assessment

  • @ref: 7306
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7306
  • description: Alicyclobacillus sacchari gene for 16S ribosomal RNA, partial sequence
  • accession: AB264020
  • length: 1518
  • database: ena
  • NCBI tax ID: 392010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alicyclobacillus sacchari DSM 17974GCA_004366795scaffoldncbi392010
66792Alicyclobacillus sacchari strain DSM 17974392010.3wgspatric392010
66792Alicyclobacillus sacchari DSM 179742756170229permanentimg392010

GC content

  • @ref: 7306
  • GC-content: 56.6

External links

@ref: 7306

culture collection no.: DSM 17974, IAM 15230

straininfo link

@refpassport
20218http://www.straininfo.net/strains/720519
20218http://www.straininfo.net/strains/720520

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7306Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17974)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17974
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2819910.1099/ijs.0.64692-0
31943Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information28199
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)