Strain identifier

BacDive ID: 4269

Type strain: Yes

Species: Arhodomonas aquaeolei

Strain Designation: HA-1

Strain history: CIP <- 1993, ATCC <- J.O. Adkins: strain HA-1 <- W. Jenkins

NCBI tax ID(s): 1121016 (strain), 2369 (species)

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General

@ref: 3447

BacDive-ID: 4269

DSM-Number: 8974

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile

description: Arhodomonas aquaeolei HA-1 is a mesophilic, motile bacterium that was isolated from oil field brine.

NCBI tax id

NCBI tax idMatching level
1121016strain
2369species

strain history

@refhistory
3447<- ATCC <- J. P. Adkins <- C. D. Jenkins; HA-1
116425CIP <- 1993, ATCC <- J.O. Adkins: strain HA-1 <- W. Jenkins

doi: 10.13145/bacdive4269.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Ectothiorhodospiraceae
  • genus: Arhodomonas
  • species: Arhodomonas aquaeolei
  • full scientific name: Arhodomonas aquaeolei Adkins et al. 1993

@ref: 3447

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Ectothiorhodospiraceae

genus: Arhodomonas

species: Arhodomonas aquaeolei

full scientific name: Arhodomonas aquaeolei Adkins et al. 1993

strain designation: HA-1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.39
69480100negative
116425yesnegativerod-shaped

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_8974_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_8974_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_8974_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_8974_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_8974_5.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_8974_6.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3447BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
3447MEDIUM FOR ARHODOMONAS AND MARINOBACTER (DSMZ Medium 941)yeshttps://mediadive.dsmz.de/medium/941Name: MEDIUM FOR ARHODOMONAS AND MARINOBACTER (DSMZ Medium 941) Composition: NaCl 6.0 g/l NH4Cl 1.0 g/l Na-acetate 1.0 g/l MgSO4 x 7 H2O 0.2 g/l KCl 0.1 g/l KH2PO4 0.1 g/l Bacto peptone 0.1 g/l CaCl2 x 2 H2O 0.04 g/l HCl 0.001625 g/l FeCl2 x 7 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l H3BO3 6.2e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l CuCl2 x 2 H2O 1.7e-05 g/l Pyridoxine hydrochloride 1e-05 g/l (DL)-alpha-Lipoic acid 5e-06 g/l Calcium D-(+)-pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine HCl 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water
34431MEDIUM 86 - for Arhodomonas aquaeoleiyesDistilled water make up to (1000.000 ml);Sodium chloride (150.000 g);Agar (20.000 g);Potassium nitrate (2.000 g);Plate count broth (17.000 g)
116425CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
3447positivegrowth37mesophilic
34431positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11642516947citrate-carbon source
11642517632nitrate+reduction
11642516301nitrite-reduction

metabolite production

  • @ref: 116425
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 116425
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
116425oxidase+
116425alcohol dehydrogenase-1.1.1.1
116425gelatinase-
116425catalase+1.11.1.6
116425lysine decarboxylase-4.1.1.18
116425ornithine decarboxylase-4.1.1.17
116425urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3447oil field brine
116425Oil field brineOklahomaUnited States of AmericaUSANorth America1987

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Oil reservoir
#Condition#Saline

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
34471Risk group (German classification)
1164251Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3447
  • description: Arhodomonas aquaeolei strain ATCC 49307 small subunit ribosomal RNA gene, complete sequence
  • accession: M26631
  • length: 1485
  • database: ena
  • NCBI tax ID: 2369

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arhodomonas aquaeolei DSM 8974GCA_000374645scaffoldncbi1121016
66792Arhodomonas aquaeolei DSM 89741121016.3wgspatric1121016
66792Arhodomonas aquaeolei DSM 89742519899679draftimg1121016

GC content

  • @ref: 3447
  • GC-content: 67

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno84no
motileyes91.29no
flagellatedyes73.255no
gram-positiveno98.548no
anaerobicno92.249no
aerobicyes65.203no
halophileyes81.644no
spore-formingno94.088no
glucose-utilyes72.554no
thermophileno95.774yes
glucose-fermentno88.127no

External links

@ref: 3447

culture collection no.: DSM 8974, ATCC 49307, CIP 103913

straininfo link

  • @ref: 73763
  • straininfo: 42415

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8347510Arhodomonas aquaeolei gen. nov., sp. nov., an aerobic, halophilic bacterium isolated from a subterranean brine.Adkins JP, Madigan MT, Mandelco L, Woese CR, Tanner RSInt J Syst Bacteriol10.1099/00207713-43-3-5141993Bacterial Typing Techniques, Base Sequence, Biotin/metabolism, Carboxylic Acids/metabolism, Catalase/analysis, Flagella, Gram-Negative Aerobic Bacteria/*classification/genetics/*isolation & purification/ultrastructure, Industrial Microbiology, Molecular Sequence Data, Petroleum, RNA, Ribosomal, 16S/genetics, Salts, Sodium ChlorideMetabolism
Genetics25149520Arhodomonas sp. strain Seminole and its genetic potential to degrade aromatic compounds under high-salinity conditions.Dalvi S, Nicholson C, Najar F, Roe BA, Canaan P, Hartson SD, Fathepure BZAppl Environ Microbiol10.1128/AEM.01509-142014Chromatography, Liquid, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ectothiorhodospiraceae/drug effects/*genetics/growth & development/*metabolism, Genome, Bacterial, Hydrocarbons, Aromatic/*metabolism, Mass Spectrometry, Metabolic Networks and Pathways/*genetics, Molecular Sequence Data, Multigene Family, Phylogeny, Proteome/analysis, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNAPhylogeny
Phylogeny26394793Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern.Leon MJ, Rodriguez-Olmos A, Sanchez-Porro C, Lopez-Perez M, Rodriguez-Valera F, Soliveri J, Ventosa A, Copa-Patino JLInt J Syst Evol Microbiol10.1099/ijsem.0.0006212015Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Spain, *Water MicrobiologyGenetics
Phylogeny27470064Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns.Leon MJ, Vera-Gargallo B, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0013382016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Spain, *Water MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3447Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8974)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8974
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34431Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15763
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
73763Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42415.1StrainInfo: A central database for resolving microbial strain identifiers
116425Curators of the CIPCollection of Institut Pasteur (CIP 103913)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103913