Strain identifier
BacDive ID: 42
Type strain:
Species: Gluconacetobacter sacchari
Strain history: CIP <- 2000, ACM <- 1995, I. Franke, Queensland Univ., Australia: strain SRI 1794
NCBI tax ID(s): 1307940 (strain), 92759 (species)
General
@ref: 4795
BacDive-ID: 42
DSM-Number: 12717
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Gluconacetobacter sacchari DSM 12717 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from mealy bug from sugar cane.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1307940 | strain |
92759 | species |
strain history
@ref | history |
---|---|
4795 | <- I. H. Franke, Univ. Queensland, Brisbane; SRI1794 |
122330 | CIP <- 2000, ACM <- 1995, I. Franke, Queensland Univ., Australia: strain SRI 1794 |
doi: 10.13145/bacdive42.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Rhodospirillales
- family: Acetobacteraceae
- genus: Gluconacetobacter
- species: Gluconacetobacter sacchari
- full scientific name: Gluconacetobacter sacchari Franke et al. 1999
@ref: 4795
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Acetobacteraceae
genus: Gluconacetobacter
species: Gluconacetobacter sacchari
full scientific name: Gluconacetobacter sacchari Franke et al. 1999
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement |
---|---|---|---|---|---|---|
23243 | negative | 1.3-2.2 µm | 0.7-0.9 µm | rod-shaped | yes | peritrichous |
122330 | negative | rod-shaped | yes |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
23243 | khaki-brown | 3-4 days | WL nutrient agar |
23243 | brown | GYC |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4795 | SABOURAND GLUCOSE MEDIUM (DSMZ Medium 1429) | yes | https://mediadive.dsmz.de/medium/1429 | Name: SABOURAUD GLUCOSE MEDIUM (DSMZ Medium 1429) Composition: SABOURAUD- Glucose-Bouillon 30.0 g/l Agar 15.0 g/l Distilled water |
4795 | GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) | yes | https://mediadive.dsmz.de/medium/105 | Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water |
23243 | GYC | yes | 50 g glucose, 10 g yeast extract, 0 g calcium carbonate and 25 g agar per 1l distilled water | |
23243 | WL nutrient agar | yes | 4 g yeast extract, 4 g tryptone, 50 g glucose, 0.55 g K2HPO4, 0.425 g KCl, 0.125 g CaCl2, 0.125 g MgSO4, 0.0025 g FeCl3, 0.0025 g MnSO4, 0.022 g bromocresol green and 15 g agar per 1l distilled water | |
39554 | MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g) | |
122330 | CIP Medium 314 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314 | |
122330 | CIP Medium 1 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4795 | positive | growth | 28 | mesophilic |
39554 | positive | growth | 30 | mesophilic |
122330 | positive | growth | 22-30 | |
122330 | no | growth | 10 | psychrophilic |
122330 | no | growth | 37 | mesophilic |
122330 | no | growth | 41 | thermophilic |
122330 | no | growth | 45 | thermophilic |
Physiology and metabolism
tolerance
@ref | compound | percentage |
---|---|---|
23243 | Glucose | 30 |
23243 | Malachite green | 0.01 |
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23243 | obligate aerobe |
122330 | obligate aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
122330 | NaCl | positive | growth | 0 % |
122330 | NaCl | no | growth | 2 % |
122330 | NaCl | no | growth | 4 % |
122330 | NaCl | no | growth | 6 % |
122330 | NaCl | no | growth | 8 % |
122330 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23243 | 16899 | D-mannitol | - | builds acid from |
23243 | 30911 | sorbitol | - | builds acid from |
23243 | 16813 | galactitol | - | carbon source |
23243 | 15428 | glycine | - | carbon source |
23243 | 17196 | L-asparagine | - | carbon source |
23243 | 18050 | L-glutamine | - | carbon source |
23243 | 16857 | L-threonine | - | carbon source |
23243 | 16828 | L-tryptophan | - | carbon source |
23243 | 17790 | methanol | - | carbon source |
23243 | 27518 | acetylene | - | reduction |
23243 | 17632 | nitrate | - | reduction |
23243 | 17234 | glucose | + | builds acid from |
23243 | 28831 | propanol | + | builds acid from |
23243 | 30089 | acetate | + | carbon source |
23243 | 16236 | ethanol | + | carbon source |
23243 | 29987 | glutamate | + | growth |
23243 | 29864 | mannitol | + | growth |
23243 | 30089 | acetate | + | oxidation |
23243 | 16236 | ethanol | + | oxidation |
23243 | 24996 | lactate | + | oxidation |
122330 | 606565 | hippurate | - | hydrolysis |
122330 | 17632 | nitrate | + | reduction |
122330 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23243 | 16136 | hydrogen sulfide | no |
23243 | 17426 | 5-dehydro-D-gluconate | yes |
23243 | 16808 | 2-dehydro-D-gluconate | yes |
122330 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
122330 | oxidase | - | |
122330 | beta-galactosidase | - | 3.2.1.23 |
122330 | gelatinase | - | |
122330 | catalase | + | 1.11.1.6 |
122330 | gamma-glutamyltransferase | - | 2.3.2.2 |
122330 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122330 | - | - | + | + | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
122330 | - | + | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | isolation procedure | isolation date |
---|---|---|---|---|---|---|---|---|---|
4795 | mealy bug from sugar cane | Queensland | Australia | AUS | Australia and Oceania | ||||
23243 | leaf sheath of sugar cane and pink sugar-cane mealy bug (Saccharicoccus sacchari) | WL nutrient agar | add 100 mg/L cycloheximide | Serial dilutions plated | |||||
122330 | Sugar cane | Australia | AUS | Australia and Oceania | 1984 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Disease | |
#Host Body-Site | #Plant | #Leaf (Phyllosphere) |
#Host | #Arthropoda | #Insecta |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
taxonmaps
- @ref: 69479
- File name: preview.99_126865.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_64;97_2865;98_3566;99_126865&stattab=map
- Last taxonomy: Gluconacetobacter
- 16S sequence: JF794008
- Sequence Identity:
- Total samples: 7
- aquatic counts: 1
- animal counts: 6
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4795 | 1 | Risk group (German classification) |
122330 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Gluconacetobacter sacchari strain DSM 12717 16S ribosomal RNA gene, partial sequence | JF794008 | 1364 | ena | 92759 |
4795 | Gluconacetobacter sacchari isolate SRI1794 16S ribosomal RNA gene, partial sequence | AF127407 | 1427 | ena | 92759 |
GC content
- @ref: 4795
- GC-content: 65
- method: high performance liquid chromatography (HPLC)
External links
@ref: 4795
culture collection no.: DSM 12717, SRI 1794, CIP 106693
straininfo link
- @ref: 69727
- straininfo: 68639
literature
- topic: Phylogeny
- Pubmed-ID: 10555349
- title: Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug.
- authors: Franke IH, Fegan M, Hayward C, Leonard G, Stackebrandt E, Sly LI
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-49-4-1681
- year: 1999
- mesh: Acetobacteraceae/chemistry/*classification/isolation & purification/physiology, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Insecta/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Leaves/microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4795 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12717) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12717 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23243 | Ingrid H. Franke, Mark Fegan, Chris Hayward, Graham Leonard, Erko Stackebrandt, Lindsay I. Sly | 10.1099/00207713-49-4-1681 | Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug | IJSEM 49: 1681-1693 1999 | 10555349 | |
39554 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18851 | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69727 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID68639.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
122330 | Curators of the CIP | Collection of Institut Pasteur (CIP 106693) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106693 |