Strain identifier

BacDive ID: 42

Type strain: Yes

Species: Gluconacetobacter sacchari

Strain history: CIP <- 2000, ACM <- 1995, I. Franke, Queensland Univ., Australia: strain SRI 1794

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General

@ref: 4795

BacDive-ID: 42

DSM-Number: 12717

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Gluconacetobacter sacchari DSM 12717 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from mealy bug from sugar cane.

NCBI tax id

NCBI tax idMatching level
1307940strain
92759species

strain history

@refhistory
4795<- I. H. Franke, Univ. Queensland, Brisbane; SRI1794
122330CIP <- 2000, ACM <- 1995, I. Franke, Queensland Univ., Australia: strain SRI 1794

doi: 10.13145/bacdive42.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Gluconacetobacter
  • species: Gluconacetobacter sacchari
  • full scientific name: Gluconacetobacter sacchari Franke et al. 1999

@ref: 4795

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Gluconacetobacter

species: Gluconacetobacter sacchari

full scientific name: Gluconacetobacter sacchari Franke et al. 1999

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23243negative1.3-2.2 µm0.7-0.9 µmrod-shapedyesperitrichous
122330negativerod-shapedyes

colony morphology

@refcolony colorincubation periodmedium used
23243khaki-brown3-4 daysWL nutrient agar
23243brownGYC

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4795SABOURAND GLUCOSE MEDIUM (DSMZ Medium 1429)yeshttps://mediadive.dsmz.de/medium/1429Name: SABOURAUD GLUCOSE MEDIUM (DSMZ Medium 1429) Composition: SABOURAUD- Glucose-Bouillon 30.0 g/l Agar 15.0 g/l Distilled water
4795GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)yeshttps://mediadive.dsmz.de/medium/105Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water
23243GYCyes50 g glucose, 10 g yeast extract, 0 g calcium carbonate and 25 g agar per 1l distilled water
23243WL nutrient agaryes4 g yeast extract, 4 g tryptone, 50 g glucose, 0.55 g K2HPO4, 0.425 g KCl, 0.125 g CaCl2, 0.125 g MgSO4, 0.0025 g FeCl3, 0.0025 g MnSO4, 0.022 g bromocresol green and 15 g agar per 1l distilled water
39554MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
122330CIP Medium 314yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314
122330CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
4795positivegrowth28mesophilic
39554positivegrowth30mesophilic
122330positivegrowth22-30
122330nogrowth10psychrophilic
122330nogrowth37mesophilic
122330nogrowth41thermophilic
122330nogrowth45thermophilic

Physiology and metabolism

tolerance

@refcompoundpercentage
23243Glucose30
23243Malachite green0.01

oxygen tolerance

@refoxygen tolerance
23243obligate aerobe
122330obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122330NaClpositivegrowth0 %
122330NaClnogrowth2 %
122330NaClnogrowth4 %
122330NaClnogrowth6 %
122330NaClnogrowth8 %
122330NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2324316899D-mannitol-builds acid from
2324330911sorbitol-builds acid from
2324316813galactitol-carbon source
2324315428glycine-carbon source
2324317196L-asparagine-carbon source
2324318050L-glutamine-carbon source
2324316857L-threonine-carbon source
2324316828L-tryptophan-carbon source
2324317790methanol-carbon source
2324327518acetylene-reduction
2324317632nitrate-reduction
2324317234glucose+builds acid from
2324328831propanol+builds acid from
2324330089acetate+carbon source
2324316236ethanol+carbon source
2324329987glutamate+growth
2324329864mannitol+growth
2324330089acetate+oxidation
2324316236ethanol+oxidation
2324324996lactate+oxidation
122330606565hippurate-hydrolysis
12233017632nitrate+reduction
12233016301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2324316136hydrogen sulfideno
23243174265-dehydro-D-gluconateyes
23243168082-dehydro-D-gluconateyes
12233035581indoleno

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
122330oxidase-
122330beta-galactosidase-3.2.1.23
122330gelatinase-
122330catalase+1.11.1.6
122330gamma-glutamyltransferase-2.3.2.2
122330urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122330--++-+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122330-+----------+++------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionisolation procedureisolation date
4795mealy bug from sugar caneQueenslandAustraliaAUSAustralia and Oceania
23243leaf sheath of sugar cane and pink sugar-cane mealy bug (Saccharicoccus sacchari)WL nutrient agaradd 100 mg/L cycloheximideSerial dilutions plated
122330Sugar caneAustraliaAUSAustralia and Oceania1984

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Plant#Leaf (Phyllosphere)
#Host#Arthropoda#Insecta
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_126865.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_64;97_2865;98_3566;99_126865&stattab=map
  • Last taxonomy: Gluconacetobacter
  • 16S sequence: JF794008
  • Sequence Identity:
  • Total samples: 7
  • aquatic counts: 1
  • animal counts: 6

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
47951Risk group (German classification)
1223301Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Gluconacetobacter sacchari strain DSM 12717 16S ribosomal RNA gene, partial sequenceJF7940081364ena92759
4795Gluconacetobacter sacchari isolate SRI1794 16S ribosomal RNA gene, partial sequenceAF1274071427ena92759

GC content

  • @ref: 4795
  • GC-content: 65
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4795

culture collection no.: DSM 12717, SRI 1794, CIP 106693

straininfo link

  • @ref: 69727
  • straininfo: 68639

literature

  • topic: Phylogeny
  • Pubmed-ID: 10555349
  • title: Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug.
  • authors: Franke IH, Fegan M, Hayward C, Leonard G, Stackebrandt E, Sly LI
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-4-1681
  • year: 1999
  • mesh: Acetobacteraceae/chemistry/*classification/isolation & purification/physiology, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Insecta/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Plant Leaves/microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4795Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12717)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12717
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23243Ingrid H. Franke, Mark Fegan, Chris Hayward, Graham Leonard, Erko Stackebrandt, Lindsay I. Sly10.1099/00207713-49-4-1681Description of Gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bugIJSEM 49: 1681-1693 199910555349
39554Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18851
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69727Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID68639.1StrainInfo: A central database for resolving microbial strain identifiers
122330Curators of the CIPCollection of Institut Pasteur (CIP 106693)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106693