Strain identifier
BacDive ID: 4195
Type strain: 
Species: Aeropyrum pernix
Strain Designation: K1
Strain history: N. Nomura K1.
NCBI tax ID(s): 56636 (species)
version 8.1 (current version)
General
@ref: 4521
BacDive-ID: 4195
DSM-Number: 11879
keywords: genome sequence, 16S sequence, Archaea, aerobe, heterotroph, hyperthermophilic, Gram-negative, motile, coccus-shaped, colony-forming
description: Aeropyrum pernix K1 is an aerobe, heterotroph, hyperthermophilic archaeon that forms irregular colonies and was isolated from marine solfataric vent.
NCBI tax id
- NCBI tax id: 56636
- Matching level: species
strain history
| @ref | history |
|---|---|
| 4521 | <- JCM; JCM 9820 <- N. Nomura, Kyoto Univ., Japan; K1 |
| 67770 | N. Nomura K1. |
doi: 10.13145/bacdive4195.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Desulfurococcales
- family: Desulfurococcaceae
- genus: Aeropyrum
- species: Aeropyrum pernix
- full scientific name: Aeropyrum pernix Sako et al. 1996
@ref: 4521
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Desulfurococcales
family: Desulfurococcaceae
genus: Aeropyrum
species: Aeropyrum pernix
full scientific name: Aeropyrum pernix Sako et al. 1996
strain designation: K1
type strain: yes
Morphology
cell morphology
- @ref: 23222
- gram stain: negative
- cell length: 0.8-1.0 µm
- cell shape: coccus-shaped
- motility: yes
colony morphology
- @ref: 23222
- colony color: brown
- colony shape: irregular
Culture and growth conditions
culture medium
- @ref: 4521
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l Na2S2O3 x 5 H2O 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 4521 | positive | growth | 90 | hyperthermophilic |
| 23222 | positive | growth | 70-100 | hyperthermophilic |
| 23222 | no | growth | 68 | thermophilic |
| 23222 | no | growth | 102 | hyperthermophilic |
| 23222 | positive | optimum | 90-95 | hyperthermophilic |
| 67770 | positive | growth | 90 | hyperthermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 23222 | positive | growth | 5-9 | alkaliphile |
| 23222 | positive | optimum | 7.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 23222
- oxygen tolerance: aerobe
nutrition type
- @ref: 23222
- type: heterotroph
observation
- @ref: 67770
- observation: quinones: DMK-6(Hn), MTK-6(Hn)
metabolite utilization
- @ref: 23222
- Chebi-ID: 16094
- metabolite: thiosulfate
- utilization activity: +
- kind of utilization tested: growth
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 4521 | marine solfataric vent | Kodakara-Jima Island | Japan | JPN | Asia |
| 23222 | hot sedimentary materials and venting waters at a coastal solfataric vent | ||||
| 67770 | Coastal solfataric vent at Kodakara-Jima Island | Japan | JPN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Condition | #Sulfuric | |
| #Condition | #Thermophilic (>45°C) | |
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Volcanic |
Safety information
risk assessment
- @ref: 4521
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 4521 | Aeropyrum pernix gene for 16S rRNA, partial sequence | D83259 | 1444 | ena | 56636 |
| 67770 | Aeropyrum pernix genes for 16S rRNA, 23S rRNA and rRNA intron-encoded homing endonuclease, complete cds | AB008745 | 10777 | ena | 56636 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Aeropyrum pernix K1 | GCA_000011125 | complete | ncbi | 272557 |
| 66792 | Aeropyrum pernix K1 | 2923402010 | complete | img | 272557 |
| 66792 | Aeropyrum pernix K1 | 272557.25 | complete | patric | 272557 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 4521 | 67 | high performance liquid chromatography (HPLC) |
| 23222 | 67.0 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 57.388 | yes |
| flagellated | no | 93.948 | no |
| gram-positive | no | 96.458 | yes |
| anaerobic | yes | 80.312 | yes |
| halophile | no | 61.253 | no |
| spore-forming | no | 96.572 | no |
| thermophile | yes | 99.999 | no |
| glucose-util | yes | 79.971 | no |
| aerobic | no | 70.343 | no |
| glucose-ferment | no | 78.05 | no |
External links
@ref: 4521
culture collection no.: DSM 11879, JCM 9820, ATCC 700893, NBRC 100138
straininfo link
- @ref: 73695
- straininfo: 44195
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 8863437 | Aeropyrum pernix gen. nov., sp. nov., a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100 degrees C. | Sako Y, Nomura N, Uchida A, Ishida Y, Morii H, Koga Y, Hoaki T, Maruyama T | Int J Syst Bacteriol | 10.1099/00207713-46-4-1070 | 1996 | Archaea/*classification/isolation & purification/physiology, Base Sequence, DNA, Ribosomal/chemistry, Lipids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics | Genetics |
| Enzymology | 9357994 | An extremely heat-stable extracellular proteinase (aeropyrolysin) from the hyperthermophilic archaeon Aeropyrum pernix K1. | Sako Y, Croocker PC, Ishida Y | FEBS Lett | 10.1016/s0014-5793(97)01153-8 | 1997 | Caseins/metabolism, Chelating Agents/pharmacology, Crenarchaeota/*enzymology, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Hot Temperature, Hydrogen-Ion Concentration, Metalloendopeptidases/antagonists & inhibitors/chemistry/*isolation & purification/*metabolism, Molecular Weight, Protease Inhibitors/pharmacology, Protein Denaturation, Substrate Specificity | Metabolism |
| Enzymology | 10086839 | Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1. | Chavez Croocker P, Sako Y, Uchida A | Extremophiles | 10.1007/s007920050093 | 1999 | Amino Acid Sequence, Archaea/*enzymology, Chromatography, Gel, Enzyme Stability, Hot Temperature, Hydrogen-Ion Concentration, Protease Inhibitors/pharmacology, Protein Denaturation, Serine Endopeptidases/chemistry/*isolation & purification/metabolism, Substrate Specificity | Stress |
| Enzymology | 11139075 | Glutamate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix K1: enzymatic characterization, identification of the encoding gene, and phylogenetic implications. | Bhuiya MW, Sakuraba H, Kujo C, Nunoura-Kominato N, Kawarabayasi Y, Kikuchi H, Ohshima T | Extremophiles | 10.1007/s007920070002 | 2000 | Amino Acid Sequence, Cloning, Molecular, Desulfurococcaceae/*enzymology/genetics, Enzyme Stability, Glutamate Dehydrogenase/chemistry/*genetics/isolation & purification/*metabolism, Hydrogen-Ion Concentration, Kinetics, Molecular Sequence Data, Molecular Weight, Phylogeny, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Sequence Homology, Amino Acid, Substrate Specificity, Temperature | Phylogeny |
| Enzymology | 11846799 | Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1. | Han JS, Kosugi Y, Ishida H, Ishikawa K | Eur J Biochem | 10.1046/j.0014-2956.2001.02731.x | 2002 | Amino Acid Sequence, Cloning, Molecular, Crenarchaeota/*enzymology/genetics, Dihydroxyacetone Phosphate/metabolism, Escherichia coli/genetics, Glycerolphosphate Dehydrogenase/*genetics/isolation & purification/*metabolism, Kinetics, Molecular Sequence Data, Molecular Weight, NAD/metabolism, NADP/metabolism, Sequence Homology, Amino Acid, Substrate Specificity, Temperature | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4521 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11879) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11879 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23222 | YOSHIHIKO SAKO, NORIMICHI NOMURA, ARITSUNE UCHIDA, YUZABURO ISHIDA, HIROYUKI MORII, YOSUKE KOGA, TOSHIHIRO HOAKI, TADASHI MARUYAMA | 10.1099/00207713-46-4-1070 | Aeropyrum pernix gen. nov., sp. nov., a Novel Aerobic Hyperthermophilic Archaeon Growing at Temperatures up to 100°C | IJSEM 46: 1070-1077 1996 | 8863437 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 73695 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44195.1 | StrainInfo: A central database for resolving microbial strain identifiers |