Strain identifier
BacDive ID: 4170
Type strain:
Species: Fundidesulfovibrio butyratiphilus
Strain Designation: BSY
Strain history: A. Ueki strain BSY.
NCBI tax ID(s): 442866 (species)
General
@ref: 15794
BacDive-ID: 4170
DSM-Number: 21556
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Fundidesulfovibrio butyratiphilus BSY is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from anaerobic municipal sewage sludge digester.
NCBI tax id
- NCBI tax id: 442866
- Matching level: species
strain history
@ref | history |
---|---|
15794 | <- A. Ueki, Yamagata Univ., Tsuruoka, Japan; BSY <- A. Ueki {1998} |
67770 | A. Ueki strain BSY. |
doi: 10.13145/bacdive4170.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfovibrionales
- family: Desulfovibrionaceae
- genus: Fundidesulfovibrio
- species: Fundidesulfovibrio butyratiphilus
- full scientific name: Fundidesulfovibrio butyratiphilus (Suzuki et al. 2010) Galushko and Kuever 2021
synonyms
- @ref: 20215
- synonym: Desulfovibrio butyratiphilus
@ref: 15794
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfovibrionales
family: Desulfovibrionaceae
genus: Fundidesulfovibrio
species: Fundidesulfovibrio butyratiphilus
full scientific name: Fundidesulfovibrio butyratiphilus
strain designation: BSY
type strain: yes
Morphology
cell morphology
- @ref: 23217
- gram stain: negative
- cell length: 2.4-5.6 µm
- cell width: 0.8-0.9 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: polar
colony morphology
@ref | incubation period | colony color |
---|---|---|
15794 | 3-7 days | |
23217 | greyish |
Culture and growth conditions
culture medium
- @ref: 15794
- name: DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194)
- growth: yes
- link: https://mediadive.dsmz.de/medium/194
- composition: Name: DESULFOBULBUS SP. MEDIUM (FRESHWATER) (DSMZ Medium 194; with strain-specific modifications) Composition: Na2SO4 2.99103 g/l Na2CO3 1.49551 g/l Na-butyrate 0.997009 g/l NaCl 0.997009 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l MgCl2 x 6 H2O 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15794 | positive | growth | 30 | mesophilic |
23217 | positive | growth | 25-40 | |
23217 | positive | optimum | 35 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
23217 | positive | growth | 6.2-8.0 |
23217 | positive | optimum | 7.1 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
15794 | anaerobe |
23217 | anaerobe |
spore formation
- @ref: 23217
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23217 | NaCl | positive | growth | 0-2.0 %(w/v) |
23217 | NaCl | positive | optimum | 0.5 %(w/v) |
observation
- @ref: 67770
- observation: quinones: MK-6(H2)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23217 | 29806 | fumarate | - | electron acceptor |
23217 | 17632 | nitrate | - | electron acceptor |
23217 | 17359 | sulfite | - | electron acceptor |
23217 | 15740 | formate | - | electron donor |
23217 | 29806 | fumarate | - | electron donor |
23217 | 25115 | malate | - | electron donor |
23217 | 30031 | succinate | - | electron donor |
23217 | 16189 | sulfate | + | electron acceptor |
23217 | 16094 | thiosulfate | + | electron acceptor |
23217 | 17968 | butyrate | + | electron donor |
23217 | 28831 | propanol | + | electron donor |
23217 | 16189 | sulfate | + | reduction |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23217 | catalase | - | 1.11.1.6 |
23217 | cytochrome oxidase | - | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15794 | anaerobic municipal sewage sludge digester | Yokohama | Japan | JPN | Asia |
67770 | Anaerobic sewage sludge digester in Yokohama | Japan | JPN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | #Anaerobic digestor |
#Engineered | #Waste | #Sewage sludge |
#Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 15794
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
15794 | Desulfovibrio butyratiphilus gene for 16S ribosomal RNA, partial sequence, strain: BSY | AB303305 | 1450 | ena | 442866 |
67770 | Desulfovibrio butyratiphilus gene for 16S ribosomal RNA, partial sequence, strain: BST | AB303302 | 1286 | ena | 442866 |
GC content
- @ref: 15794
- GC-content: 63.3
- method: high performance liquid chromatography (HPLC)
External links
@ref: 15794
culture collection no.: DSM 21556, JCM 15519
straininfo link
- @ref: 73672
- straininfo: 398940
literature
- topic: Phylogeny
- Pubmed-ID: 19654341
- title: Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester.
- authors: Suzuki D, Ueki A, Shizuku T, Ohtaki Y, Ueki K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.013771-0
- year: 2009
- mesh: Anaerobiosis, Butyrates/*metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Desulfovibrio/*classification/genetics/*isolation & purification/metabolism, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Sulfates/*metabolism
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15794 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21556) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21556 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23217 | Daisuke Suzuki, Atsuko Ueki, Toshiko Shizuku, Yoshimi Ohtaki, Katsuji Ueki | 10.1099/ijs.0.013771-0 | Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester | IJSEM 60: 595-602 2010 | 19654341 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
73672 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID398940.1 | StrainInfo: A central database for resolving microbial strain identifiers |