Strain identifier
BacDive ID: 4164
Type strain: 
Species: Pseudodesulfovibrio tunisiensis
Strain history: Z. Ben Ali Gam RB22.
NCBI tax ID(s): 1121465 (strain), 463192 (species)
version 8.1 (current version)
General
@ref: 7992
BacDive-ID: 4164
DSM-Number: 19275
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Pseudodesulfovibrio tunisiensis DSM 19275 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from exhaust water of oil refinery.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1121465 | strain |
| 463192 | species |
strain history
| @ref | history |
|---|---|
| 7992 | <- Z. Ben Ali Gam; RB22 |
| 67770 | Z. Ben Ali Gam RB22. |
doi: 10.13145/bacdive4164.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfovibrionales
- family: Desulfovibrionaceae
- genus: Pseudodesulfovibrio
- species: Pseudodesulfovibrio tunisiensis
- full scientific name: Pseudodesulfovibrio tunisiensis (Ben Ali Gam et al. 2009) Waite et al. 2020
synonyms
- @ref: 20215
- synonym: Desulfovibrio tunisiensis
@ref: 7992
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfovibrionales
family: Desulfovibrionaceae
genus: Pseudodesulfovibrio
species: Pseudodesulfovibrio tunisiensis
full scientific name: Pseudodesulfovibrio tunisiensis (Ben Ali Gam et al. 2009) Waite et al. 2020
type strain: yes
Morphology
cell morphology
- @ref: 28900
- gram stain: negative
- cell length: 1.75 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 60521
- incubation period: 7 days
pigmentation
- @ref: 28900
- production: no
Culture and growth conditions
culture medium
- @ref: 7992
- name: DESULFOBACTER SP. MEDIUM (LACTATE) (DSMZ Medium 195c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/195c
- composition: Name: DESULFOBACTER SP. MEDIUM (LACTATE) (DSMZ Medium 195c; with strain-specific modifications) Composition: NaCl 20.9372 g/l Na2SO4 2.99103 g/l MgCl2 x 6 H2O 2.99103 g/l Na-L-lactate 2.49252 g/l Na2CO3 1.49551 g/l Yeast extract 0.997009 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Nicotinic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7992 | positive | growth | 35 | mesophilic |
| 28900 | positive | growth | 15-45 | |
| 28900 | positive | optimum | 37 | |
| 60521 | positive | growth | 37 | |
| 67770 | positive | growth | 37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 28900 | positive | growth | 4.5-9 | alkaliphile |
| 28900 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 7992 | anaerobe |
| 28900 | anaerobe |
| 60521 | anaerobe |
spore formation
- @ref: 28900
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 28900 | NaCl | positive | growth | 0-7 % |
| 28900 | NaCl | positive | optimum | 4 % |
observation
- @ref: 28900
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 28900 | 17754 | glycerol | + | carbon source |
| 28900 | 24996 | lactate | + | carbon source |
| 28900 | 30031 | succinate | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|---|
| 7992 | exhaust water of oil refinery | Bizerte | Tunisia | TUN | Africa | |
| 60521 | Wastewater reffinery | Tunisia | TUN | Africa | 2007-02-15 | |
| 67770 | Exhaust water of a Tunisian oil refinery |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Oil reservoir |
| #Engineered | #Waste | #Wastewater |
| #Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_125578.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_320;96_49183;97_63286;98_84316;99_125578&stattab=map
- Last taxonomy: Desulfovibrio tunisiensis subclade
- 16S sequence: EF577029
- Sequence Identity:
- Total samples: 101
- soil counts: 4
- aquatic counts: 83
- animal counts: 7
- plant counts: 7
Safety information
risk assessment
- @ref: 7992
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7992
- description: Desulfovibrio tunisiensis strain RB22 16S ribosomal RNA gene, partial sequence
- accession: EF577029
- length: 1541
- database: ena
- NCBI tax ID: 463192
GC content
| @ref | GC-content | method |
|---|---|---|
| 7992 | 59.6 | high performance liquid chromatography (HPLC) |
| 28900 | 59.6 |
External links
@ref: 7992
culture collection no.: DSM 19275, JCM 15076, NCIMB 14400, CCUG 55298, RB 22
straininfo link
| @ref | straininfo |
|---|---|
| 73665 | 408204 |
| 73666 | 408456 |
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19406793 | Desulfovibrio tunisiensis sp. nov., a novel weakly halotolerant, sulfate-reducing bacterium isolated from exhaust water of a Tunisian oil refinery. | Ben Ali Gam Z, Oueslati R, Abdelkafi S, Casalot L, Tholozan JL, Labat M | Int J Syst Evol Microbiol | 10.1099/ijs.0.000943-0 | 2009 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis/genetics, Desulfovibrio/*classification/genetics/isolation & purification/physiology, Fresh Water/*microbiology, Genes, rRNA, Genotype, Molecular Sequence Data, *Petroleum, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*pharmacology, Species Specificity, Sulfates/metabolism, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification/physiology, Tunisia | Genetics |
| Phylogeny | 32103446 | Paradesulfovibrio onnuriensis gen. nov., sp. nov., a chemolithoautotrophic sulfate-reducing bacterium isolated from the Onnuri vent field of the Indian Ocean and reclassification of Desulfovibrio senegalensis as Paradesulfovibrio senegalensis comb. nov. | Kim YJ, Yang JA, Lim JK, Park MJ, Yang SH, Lee HS, Kang SG, Lee JH, Kwon KK | J Microbiol | 10.1007/s12275-020-9376-0 | 2020 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desulfovibrio/*classification/isolation & purification, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Indian Ocean, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sulfates/metabolism | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 7992 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19275) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19275 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 28900 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25340 | 28776041 | |
| 60521 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 55298) | https://www.ccug.se/strain?id=55298 | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 73665 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID408204.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 73666 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID408456.1 | StrainInfo: A central database for resolving microbial strain identifiers |