Strain identifier
BacDive ID: 4068
Type strain:
Species: Bilophila wadsworthia
Strain Designation: RZATAU
Strain history: <- H. Laue, RZATAU
NCBI tax ID(s): 35833 (species)
General
@ref: 4167
BacDive-ID: 4068
DSM-Number: 11045
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen
description: Bilophila wadsworthia RZATAU is an anaerobe, mesophilic human pathogen that was isolated from anaerobic mud, sewage plant.
NCBI tax id
- NCBI tax id: 35833
- Matching level: species
strain history
- @ref: 4167
- history: <- H. Laue, RZATAU
doi: 10.13145/bacdive4068.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfovibrionales
- family: Desulfovibrionaceae
- genus: Bilophila
- species: Bilophila wadsworthia
- full scientific name: Bilophila wadsworthia Baron et al. 1990
@ref: 4167
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfovibrionales
family: Desulfovibrionaceae
genus: Bilophila
species: Bilophila wadsworthia
full scientific name: Bilophila wadsworthia Baron et al. 1990
strain designation: RZATAU
type strain: no
Morphology
colony morphology
- @ref: 4167
- incubation period: 3-7 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4167 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
4167 | FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) | yes | https://mediadive.dsmz.de/medium/1203 | Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water |
4167 | ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503) | yes | https://mediadive.dsmz.de/medium/503 | Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: D-Glucose 4.98504 g/l Na-formate 4.08043 g/l Na2CO3 1.49551 g/l Taurine 1.25148 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l 1,4-Naphthoquinone Distilled water |
culture temp
- @ref: 4167
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4167
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4167
- sample type: anaerobic mud, sewage plant
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Waste | #Water treatment plant |
#Environmental | #Terrestrial | #Mud (Sludge) |
#Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_2428.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_322;96_1312;97_1559;98_1896;99_2428&stattab=map
- Last taxonomy: Bilophila wadsworthia subclade
- 16S sequence: AB117562
- Sequence Identity:
- Total samples: 248687
- soil counts: 2066
- aquatic counts: 7361
- animal counts: 238547
- plant counts: 713
Safety information
risk assessment
- @ref: 4167
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4167
- description: Bilophila wadsworthia gene for 16S rRNA, partial sequence, strain:NB-12
- accession: AB117562
- length: 1445
- database: ena
- NCBI tax ID: 35833
External links
@ref: 4167
culture collection no.: DSM 11045
straininfo link
- @ref: 73571
- straininfo: 49002
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 9143131 | Taurine reduction in anaerobic respiration of Bilophila wadsworthia RZATAU. | Laue H, Denger K, Cook AM | Appl Environ Microbiol | 10.1128/aem.63.5.2016-2021.1997 | 1997 | Acetates/metabolism, Alanine/biosynthesis, Ammonia/metabolism, Anaerobiosis, Bioreactors, Cysteic Acid/metabolism, DNA, Bacterial/analysis, Formates/metabolism, Gram-Negative Anaerobic Bacteria/classification/genetics/*metabolism, Isethionic Acid/metabolism, Lactic Acid/metabolism, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Sulfates/metabolism, Sulfides/metabolism, Sulfites/metabolism, Taurine/*metabolism, Thiosulfates/metabolism | Phylogeny |
Enzymology | 11041346 | Purification, properties and primary structure of alanine dehydrogenase involved in taurine metabolism in the anaerobe Bilophila wadsworthia. | Laue H, Cook AM | Arch Microbiol | 10.1007/s002030000190 | 2000 | Alanine Dehydrogenase, *Amino Acid Oxidoreductases/chemistry/genetics/isolation & purification/metabolism, Amino Acid Sequence, Bacteria, Anaerobic/*enzymology/genetics/growth & development, Catalysis, DNA, Bacterial/genetics, Deltaproteobacteria/*enzymology/genetics/growth & development, Kinetics, Molecular Sequence Data, Sequence Analysis, DNA, Taurine/*metabolism | Metabolism |
Metabolism | 11082195 | Biochemical and molecular characterization of taurine:pyruvate aminotransferase from the anaerobe Bilophila wadsworthia. | Laue H, Cook AM | Eur J Biochem | 10.1046/j.1432-1033.2000.01782.x | 2000 | Butyrates/metabolism, Chromatography, Gel, Chromatography, High Pressure Liquid, Cysteine/metabolism, Deltaproteobacteria/*enzymology, Electrophoresis, Polyacrylamide Gel, Kinetics, Models, Chemical, Molecular Sequence Data, Molecular Weight, Open Reading Frames, Oxaloacetic Acid/metabolism, Pyridoxal Phosphate/pharmacology, Taurine/*analogs & derivatives/metabolism, Time Factors, Transaminases/*chemistry/*genetics, Ultraviolet Rays, beta-Alanine/metabolism | Enzymology |
Enzymology | 11160104 | Dissimilatory sulfite reductase (desulfoviridin) of the taurine-degrading, non-sulfate-reducing bacterium Bilophila wadsworthia RZATAU contains a fused DsrB-DsrD subunit. | Laue H, Friedrich M, Ruff J, Cook AM | J Bacteriol | 10.1128/JB.183.5.1727-1733.2001 | 2001 | Amino Acid Sequence, Anaerobiosis, Deltaproteobacteria/*enzymology/genetics/growth & development, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Oxidoreductases/metabolism, Oxidoreductases Acting on Sulfur Group Donors/*genetics/isolation & purification/*metabolism, Phylogeny, Sequence Analysis, DNA, Sulfates/metabolism, Sulfites/metabolism, Taurine/*metabolism | Metabolism |
Metabolism | 31177942 | The 'in vivo lifestyle' of bile acid 7alpha-dehydroxylating bacteria: comparative genomics, metatranscriptomic, and bile acid metabolomics analysis of a defined microbial community in gnotobiotic mice. | Ridlon JM, Devendran S, Alves JM, Doden H, Wolf PG, Pereira GV, Ly L, Volland A, Takei H, Nittono H, Murai T, Kurosawa T, Chlipala GE, Green SJ, Hernandez AG, Fields CJ, Wright CL, Kakiyama G, Cann I, Kashyap P, McCracken V, Gaskins HR | Gut Microbes | 10.1080/19490976.2019.1618173 | 2019 | Animals, Bacteria, Anaerobic/*genetics/*metabolism, Bacteroides/genetics/metabolism, Bile Acids and Salts/*metabolism, Bilophila/genetics/metabolism, Cecum/*microbiology, Cholic Acids/metabolism, Clostridium/genetics/metabolism, Gene Expression Regulation, Bacterial, Genome, Bacterial, Genomics, Germ-Free Life, Humans, *Metabolome, Mice, Mice, Inbred C57BL, Microbiota, Operon, RNA-Seq, *Transcriptome, Up-Regulation | Genetics |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
4167 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11045) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11045 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
73571 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49002.1 | StrainInfo: A central database for resolving microbial strain identifiers |