Strain identifier

BacDive ID: 4067

Type strain: Yes

Species: Desulfonatronum thiosulfatophilum

Strain Designation: ASO4-2

Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO4-2 <- D. Y. Sorokin {2007}

NCBI tax ID(s): 617002 (species)

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General

@ref: 15624

BacDive-ID: 4067

DSM-Number: 21338

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Desulfonatronum thiosulfatophilum ASO4-2 is an anaerobe, mesophilic bacterium that was isolated from sediments from hypersaline soda lake.

NCBI tax id

  • NCBI tax id: 617002
  • Matching level: species

strain history

  • @ref: 15624
  • history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO4-2 <- D. Y. Sorokin {2007}

doi: 10.13145/bacdive4067.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Desulfovibrionales
  • family: Desulfonatronaceae
  • genus: Desulfonatronum
  • species: Desulfonatronum thiosulfatophilum
  • full scientific name: Desulfonatronum thiosulfatophilum Sorokin et al. 2011

@ref: 15624

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Desulfovibrionales

family: Desulfonatronaceae

genus: Desulfonatronum

species: Desulfonatronum thiosulfatophilum

full scientific name: Desulfonatronum thiosulfatophilum Sorokin et al. 2011

strain designation: ASO4-2

type strain: yes

Morphology

colony morphology

  • @ref: 15624
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 15624
  • name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1166
  • composition: Name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166) Composition: Na2CO3 21.9342 g/l NaHCO3 7.97607 g/l NaCl 5.98205 g/l Na2S2O3 x 5 H2O 4.98504 g/l Na-formate 3.38983 g/l Na2SO4 2.79163 g/l K2HPO4 0.997009 g/l Na-pyruvate 0.548355 g/l Na2S x 9 H2O 0.239282 g/l MgCl2 x 6 H2O 0.199402 g/l NH4Cl 0.199402 g/l Yeast extract 0.0498504 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

  • @ref: 15624
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 15624
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 15624
  • sample type: sediments from hypersaline soda lake
  • geographic location: Altai, south-eastern Siberia, Kulunda Steppe, soda lake Picturesque
  • country: Russia
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Alkaline
#Condition#Saline
#Environmental#Aquatic#Non-marine Saline and Alkaline

taxonmaps

  • @ref: 69479
  • File name: preview.99_165293.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_453;96_2133;97_2569;98_108555;99_165293&stattab=map
  • Last taxonomy: Desulfonatronum thiosulfatophilum subclade
  • 16S sequence: FJ469578
  • Sequence Identity:
  • Total samples: 129
  • soil counts: 18
  • aquatic counts: 105
  • animal counts: 6

Safety information

risk assessment

  • @ref: 15624
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15624
  • description: Desulfonatronum thiosulfatophilum strain ASO4-2 16S ribosomal RNA gene, partial sequence
  • accession: FJ469578
  • length: 1522
  • database: ena
  • NCBI tax ID: 617002

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Desulfonatronum thiosulfatophilum ASO4-2GCA_900104215contigncbi617002
66792Desulfonatronum thiosulfatophilum ASO4-22596583601draftimg617002

GC content

  • @ref: 15624
  • GC-content: 57.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 15624

culture collection no.: DSM 21338, UNIQEM U757

straininfo link

  • @ref: 73570
  • straininfo: 405461

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15624Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21338)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21338
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73570Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405461.1StrainInfo: A central database for resolving microbial strain identifiers