Strain identifier
BacDive ID: 4066
Type strain: 
Species: Desulfonatronum thioautotrophicum
Strain Designation: ASO4-1
Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO4-1 <- D. Y. Sorokin {2007}
NCBI tax ID(s): 617001 (species)
version 8.1 (current version)
General
@ref: 15623
BacDive-ID: 4066
DSM-Number: 21337
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, motile
description: Desulfonatronum thioautotrophicum ASO4-1 is an anaerobe, mesophilic, motile bacterium that was isolated from sediments from hypersaline soda lake.
NCBI tax id
- NCBI tax id: 617001
- Matching level: species
strain history
- @ref: 15623
- history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ASO4-1 <- D. Y. Sorokin {2007}
doi: 10.13145/bacdive4066.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfovibrionales
- family: Desulfonatronaceae
- genus: Desulfonatronum
- species: Desulfonatronum thioautotrophicum
- full scientific name: Desulfonatronum thioautotrophicum Sorokin et al. 2011
@ref: 15623
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfovibrionales
family: Desulfonatronaceae
genus: Desulfonatronum
species: Desulfonatronum thioautotrophicum
full scientific name: Desulfonatronum thioautotrophicum Sorokin et al. 2011
strain designation: ASO4-1
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 97.663 | |
| 69480 | 99.825 | negative |
colony morphology
- @ref: 15623
- incubation period: 8-14 days
Culture and growth conditions
culture medium
- @ref: 15623
- name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1166
- composition: Name: DESULFONATRONUM THIOAUTOTROPHICUM MEDIUM (DSMZ Medium 1166) Composition: Na2CO3 21.9342 g/l NaHCO3 7.97607 g/l NaCl 5.98205 g/l Na2S2O3 x 5 H2O 4.98504 g/l Na-formate 3.38983 g/l Na2SO4 2.79163 g/l K2HPO4 0.997009 g/l Na-pyruvate 0.548355 g/l Na2S x 9 H2O 0.239282 g/l MgCl2 x 6 H2O 0.199402 g/l NH4Cl 0.199402 g/l Yeast extract 0.0498504 g/l EDTA 0.00498504 g/l FeSO4 x 7 H2O 0.00219342 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnSO4 x 7 H2O 9.97009e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l NiCl2 x 6 H2O 2.99103e-05 g/l MnCl2 x 4 H2O 2.99103e-05 g/l H3BO3 2.99103e-05 g/l CuCl2 x 2 H2O 2.99103e-05 g/l Na2MoO4 x 2 H2O 2.99103e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
- @ref: 15623
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 15623 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.429
Isolation, sampling and environmental information
isolation
- @ref: 15623
- sample type: sediments from hypersaline soda lake
- geographic location: Altai, south-eastern Siberia, Kulunda Steppe, soda lake Tanatar-1
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Alkaline | |
| #Condition | #Saline | |
| #Environmental | #Aquatic | #Non-marine Saline and Alkaline |
taxonmaps
- @ref: 69479
- File name: preview.99_165292.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_453;96_2133;97_2569;98_61041;99_165292&stattab=map
- Last taxonomy: Desulfonatronum thioautotrophicum subclade
- 16S sequence: FJ469577
- Sequence Identity:
- Total samples: 1
- aquatic counts: 1
Safety information
risk assessment
- @ref: 15623
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15623
- description: Desulfonatronum thioautotrophicum strain ASO4-1 16S ribosomal RNA gene, partial sequence
- accession: FJ469577
- length: 1496
- database: ena
- NCBI tax ID: 617001
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Desulfonatronum thioautotrophicum ASO4-1 | GCA_000934745 | scaffold | ncbi | 617001 |
| 66792 | Desulfonatronum thioautotrophicum ASO4-1 | 2593339128 | draft | img | 617001 |
GC content
- @ref: 15623
- GC-content: 57.6
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 80 | no |
| motile | yes | 88.167 | no |
| flagellated | no | 65.402 | no |
| gram-positive | no | 98.798 | no |
| anaerobic | yes | 99.725 | yes |
| aerobic | no | 98.87 | yes |
| halophile | no | 73.507 | no |
| spore-forming | no | 91.834 | no |
| glucose-util | no | 53.975 | no |
| thermophile | no | 95.949 | yes |
| glucose-ferment | no | 78.694 | no |
External links
@ref: 15623
culture collection no.: DSM 21337, UNIQEM U756
straininfo link
- @ref: 73569
- straininfo: 405261
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 15623 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21337) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21337 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 73569 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405261.1 | StrainInfo: A central database for resolving microbial strain identifiers |