Strain identifier

BacDive ID: 400

Type strain: Yes

Species: Alicyclobacillus acidoterrestris

Strain Designation: GD3B

Strain history: CIP <- 2013, DSMZ <- K. Poralla, Bacillus acidoterrestris <- B. Hippchen <- Deinhard: strain GD3B

NCBI tax ID(s): 1356854 (strain), 1450 (species)

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General

@ref: 1510

BacDive-ID: 400

DSM-Number: 3922

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, thermophilic, motile

description: Alicyclobacillus acidoterrestris GD3B is a spore-forming, thermophilic, motile bacterium that was isolated from garden soil.

NCBI tax id

NCBI tax idMatching level
1356854strain
1450species

strain history

@refhistory
1510<- Deinhard, GD3B
116022CIP <- 2013, DSMZ <- K. Poralla, Bacillus acidoterrestris <- B. Hippchen <- Deinhard: strain GD3B

doi: 10.13145/bacdive400.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Alicyclobacillaceae
  • genus: Alicyclobacillus
  • species: Alicyclobacillus acidoterrestris
  • full scientific name: Alicyclobacillus acidoterrestris (Deinhard et al. 1988) Wisotzkey et al. 1992

  • synonyms

    • @ref: 20215
    • synonym: Bacillus acidoterrestris

@ref: 1510

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Alicyclobacillaceae

genus: Alicyclobacillus

species: Alicyclobacillus acidoterrestris

full scientific name: Alicyclobacillus acidoterrestris (Deinhard et al. 1988) Wisotzkey et al. 1992

strain designation: GD3B

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes93.332
69480100positive
116022nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41589MEDIUM 313 - for Alicyclobacillus acidoterrestrisyesDistilled water make up to (500.000 ml);Magnesium sulphate heptahydrate (0.180 g);Calcium chloride dihydrate (0.660 g);Glucose (5.000 g);Yeast extract (1.000 g);Ammonium sulphate (0.200 g);Potassium di-hydrogen phosphate (3.000 g);Agar solution - M0614(50
1510ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)yesName: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/402
116022CIP Medium 313yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=313

culture temp

@refgrowthtypetemperaturerange
1510positivegrowth45thermophilic
41589positivegrowth50thermophilic
116022positivegrowth25-55
116022nogrowth10psychrophilic

culture pH

  • @ref: 116022
  • ability: no
  • type: growth
  • pH: 6

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 99.728

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1160224853esculin-hydrolysis
116022606565hippurate-hydrolysis
11602217632nitrate-reduction
11602216301nitrite-reduction

metabolite production

  • @ref: 116022
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11602215688acetoin-
11602217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
116022oxidase-
116022beta-galactosidase-3.2.1.23
116022alcohol dehydrogenase-1.1.1.1
116022catalase+1.11.1.6
116022gamma-glutamyltransferase-2.3.2.2
116022lysine decarboxylase-4.1.1.18
116022ornithine decarboxylase-4.1.1.17
116022urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116022--++-++-+-++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116022---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
1510garden soil
116022Environment, Garden soilGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
15101Risk group (German classification)
1160221Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Alicyclobacillus acidoterrestris gene for 16S ribosomal RNA, partial sequenceAB0420571514ena1450
20218Alicyclobacillus acidoterrestris strain ATCC 49025 16S ribosomal RNA gene, partial sequenceAY5737971494ena1450
20218Alicyclobacillus acidoterrestris 16S rRNA gene, strain DSM 3922TAJ1336311482ena1450
20218Alicyclobacillus acidoterrestris isolate Aaci36 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723608564ena1450
20218Alicyclobacillus acidoterrestris isolate Aaci39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723609441ena1450
20218Alicyclobacillus acidoterrestris strain DSM 3922 16S ribosomal RNA gene, partial sequenceKF8807241421ena1450
20218Bacillus sp. 16S ribosomal RNAX606021432ena1409

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alicyclobacillus acidoterrestris ATCC 49025GCA_000444055contigncbi1356854
66792Alicyclobacillus acidoterrestris ATCC 490251356854.4wgspatric1356854
66792Alicyclobacillus acidoterrestris ATCC 490252558860250draftimg1356854
66792Alicyclobacillus acidoterrestris DSM 3922GCA_022674245completencbi1450

GC content

  • @ref: 1510
  • GC-content: 51.6
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno63no
flagellatedno59.549no
gram-positiveyes80.328no
anaerobicno98.868no
aerobicyes92.637no
halophileno92.295no
spore-formingyes94.052no
motileyes84.473no
glucose-fermentno86.875no
thermophileyes98.384yes
glucose-utilyes87.932no

External links

@ref: 1510

culture collection no.: DSM 3922, ATCC 49025, CIP 106132

straininfo link

  • @ref: 70080
  • straininfo: 1091

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology20338657Kinetics models for the inactivation of Alicyclobacillus acidiphilus DSM14558(T) and Alicyclobacillus acidoterrestris DSM 3922(T) in apple juice by ultrasound.Wang J, Hu X, Wang ZInt J Food Microbiol10.1016/j.ijfoodmicro.2010.02.0292010Alicyclobacillus/*growth & development, Beverages/*microbiology, Food Contamination/prevention & control, *Food Microbiology, Linear Models, Malus/*microbiology, Microbial Viability, Models, Theoretical, *UltrasonicsEnzymology
Stress22747993Expression of DnaJ gene in Alicyclobacillus acidoterrestris under stress conditions by quantitative real-time PCR.Jiao L, Fan M, Hua C, Wang S, Wei XJ Food Sci10.1111/j.1750-3841.2012.02790.x2012Alicyclobacillus/*genetics/metabolism, Amino Acid Sequence, Cloning, Molecular, Colony Count, Microbial, DNA, Bacterial/genetics, Food Handling/methods, *Food Microbiology, Gene Expression Regulation, Bacterial/*genetics, *Genes, Bacterial, Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Stress, PhysiologicalEnzymology
Enzymology23992502Purification and characterization of anti-Alicyclobacillus bacteriocin produced by Lactobacillus rhamnosus.Yue T, Pei J, Yuan YJ Food Prot10.4315/0362-028X.JFP-12-4962013Alicyclobacillus/*drug effects/growth & development, Bacteriocins/biosynthesis/*isolation & purification/*pharmacology, Cell Membrane/*drug effects, Culture Media, Food Microbiology, Food Preservation/*methods, Lactobacillus rhamnosus/*metabolismPathogenicity
Genetics24009113Draft Genome Sequence of Alicyclobacillus acidoterrestris Strain ATCC 49025.Shemesh M, Pasvolsky R, Sela N, Green SJ, Zakin VGenome Announc10.1128/genomeA.00638-132013
Enzymology24042001UV-C light inactivation and modeling kinetics of Alicyclobacillus acidoterrestris spores in white grape and apple juices.Baysal AH, Molva C, Unluturk SInt J Food Microbiol10.1016/j.ijfoodmicro.2013.08.0152013Alicyclobacillus/*physiology/*radiation effects, Beverages/*microbiology, Food Microbiology/*methods, Hot Temperature, Kinetics, Malus/microbiology, Microbial Viability/radiation effects, *Models, Theoretical, Spores, Bacterial/*radiation effects, *Ultraviolet Rays, Vitis/microbiologyStress
Enzymology25129530Effect of sporulation medium on wet-heat resistance and structure of Alicyclobacillus acidoterrestris DSM 3922-type strain spores and modeling of the inactivation kinetics in apple juice.Molva C, Baysal AHInt J Food Microbiol10.1016/j.ijfoodmicro.2014.07.0332014Agar/chemistry, Alicyclobacillus/chemistry/*drug effects/physiology/ultrastructure, Beverages/*microbiology, Culture Media/chemistry/*pharmacology, Hot Temperature, Kinetics, Malus/*microbiology, Picolinic Acids/metabolism, Spores, Bacterial/drug effects/physiology/ultrastructure, Stress, PhysiologicalPathogenicity
25475338Study of the inactivation of spoilage microorganisms in apple juice by pulsed light and ultrasound.Ferrario M, Alzamora SM, Guerrero SFood Microbiol10.1016/j.fm.2014.06.0172014Bacteria/growth & development/radiation effects, Beverages/*microbiology, Food Preservation/instrumentation/*methods, Light, Malus/*microbiology, Ultrasonics, Yeasts/growth & development/radiation effects
Biotechnology25676243Effects of pomegranate and pomegranate-apple blend juices on the growth characteristics of Alicyclobacillus acidoterrestris DSM 3922 type strain vegetative cells and spores.Molva C, Baysal AHInt J Food Microbiol10.1016/j.ijfoodmicro.2015.01.0192015Alicyclobacillus/*growth & development/metabolism, Beverages/*microbiology, *Food Microbiology, Lythraceae/*chemistry, Malus/*chemistry, Spores, Bacterial/growth & developmentMetabolism
Phylogeny26476812Alicyclobacillus fodiniaquatilis sp. nov., isolated from acid mine water.Zhang B, Wu YF, Song JL, Huang ZS, Wang BJ, Liu SJ, Jiang CYInt J Syst Evol Microbiol10.1099/ijsem.0.0006952015Alicyclobacillus/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water MicrobiologyGenetics
26629793Evaluation of hydrophobicity and quantitative analysis of biofilm formation by Alicyclobacillus sp.Tyfa A, Kunicka-Styczynska A, Zabielska JActa Biochim Pol10.18388/abp.2015_11332015Alicyclobacillus/*physiology, Bacterial Adhesion, *Biofilms, Hydrophobic and Hydrophilic Interactions
Biotechnology28242037Influence of high power ultrasound on selected moulds, yeasts and Alicyclobacillus acidoterrestris in apple, cranberry and blueberry juice and nectar.Rezek Jambrak A, Simunek M, Evacic S, Markov K, Smoljanic G, Frece JUltrasonics10.1016/j.ultras.2017.02.0112017Bacteria/cytology/*radiation effects, Dose-Response Relationship, Radiation, Food Contamination/*prevention & control, Food Handling/methods, Food Microbiology, Fruit and Vegetable Juices/*microbiology/*radiation effects, Fungi/cytology/*radiation effects, *High-Energy Shock Waves, Radiation Dosage, Sterilization/*methods
Biotechnology28728873Inactivation of Alicyclobacillus acidoterrestris ATCC 49025 spores in apple juice by pulsed light. Influence of initial contamination and required reduction levels.Ferrario MI, Guerrero SNRev Argent Microbiol10.1016/j.ram.2017.04.0022017*Alicyclobacillus, *Beverages, Food Contamination, Food Microbiology, *Malus, *Spores, Bacterial
Pathogenicity29425865Characterization and long term antimicrobial activity of the nisin anchored cellulose films.Wu H, Teng C, Liu B, Tian H, Wang JInt J Biol Macromol10.1016/j.ijbiomac.2018.01.1942018Alicyclobacillus/drug effects, Anti-Bacterial Agents/*chemistry/*pharmacology, Cellulose/*chemistry, Food Packaging, Membranes, Artificial, Nisin/*chemistry/*pharmacology
Enzymology29706336Development and validation of predictive models for the effect of storage temperature and pH on the growth boundaries and kinetics of Alicyclobacillus acidoterrestris ATCC 49025 in fruit drinks.Kakagianni M, Kalantzi K, Beletsiotis E, Ghikas D, Lianou A, Koutsoumanis KPFood Microbiol10.1016/j.fm.2018.02.0192018Alicyclobacillus/*growth & development, Beverages/microbiology, *Food Microbiology, *Food Storage, Fruit/microbiology, Fruit and Vegetable Juices/*microbiology, *Hydrogen-Ion Concentration, Kinetics, Logistic Models, Models, Biological, Spores, Bacterial/growth & development, *TemperatureBiotechnology
Stress30704599Analysis of differential expression proteins reveals the key pathway in response to heat stress in Alicyclobacillus acidoterrestris DSM 3922(T).Feng X, He C, Jiao L, Liang X, Zhao R, Guo YFood Microbiol10.1016/j.fm.2019.01.0032019Adaptation, Physiological, Alicyclobacillus/genetics/metabolism/*physiology, Bacterial Proteins/genetics/metabolism, Gene Expression Profiling, *Gene Expression Regulation, Bacterial, *Heat-Shock Response/genetics, Hot Temperature, Metabolic Networks and Pathways/*genetics, Microbial Viability, ProteomicsMetabolism
Cultivation30714624The Role of Solid Support Bound Metal Chelators on System-Dependent Synergy and Antagonism with Nisin.Herskovitz JE, Worobo RW, Goddard JMJ Food Sci10.1111/1750-3841.144442019Alicyclobacillus/*drug effects, Anti-Infective Agents, Chelating Agents/chemistry/*pharmacology, Culture Media, Food, Food Microbiology, *Food Packaging, *Food Preservation, Food Preservatives/pharmacology, Fruit and Vegetable Juices/analysis/microbiology, Malus/chemistry, Metals/*chemistry, Nisin/pharmacologyBiotechnology
31294163Modeling growth of Alicyclobacillus acidoterrestris DSM 3922 type strain vegetative cells in the apple juice with nisin and lysozyme.Molva C, Baysal AHAIMS Microbiol10.3934/microbiol.2017.2.3152017
Metabolism31522091Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages.Van Luong TS, Moir CJ, Kaur M, Frank D, Bowman JP, Bradbury MIInt J Food Microbiol10.1016/j.ijfoodmicro.2019.1083142019Alicyclobacillus/*genetics/isolation & purification/*metabolism, DNA Fingerprinting, Fruit/*metabolism, Fruit and Vegetable Juices/*microbiology, Gas Chromatography-Mass Spectrometry, Genotype, Guaiacol/*metabolism, Polymerase Chain Reaction, Random Amplified Polymorphic DNA TechniqueGenetics
34458303Viability, Sublethal Injury, and Release of Cellular Components From Alicyclobacillus acidoterrestris Spores and Cells After the Application of Physical Treatments, Natural Extracts, or Their Components.Bevilacqua A, Petruzzi L, Speranza B, Campaniello D, Ciuffreda E, Altieri C, Sinigaglia M, Corbo MRFront Nutr10.3389/fnut.2021.7005002021
Transcriptome36240455Unveiling the complete genome sequence of Alicyclobacillus acidoterrestris DSM 3922T, a taint-producing strain.Leonardo IC, Barreto Crespo MT, Gaspar FBG3 (Bethesda)10.1093/g3journal/jkac2252022*Alicyclobacillus/genetics/metabolism, Reproducibility of Results, Fruit and Vegetable Juices, Sequence Analysis, DNAGenetics
36446270Inactivation of Alicyclobacillus acidoterrestris spores, single or composite Escherichia coli and native microbiota in isotonic fruit-flavoured sports drinks processed by UV-C light.Kozono L, Fenoglio D, Ferrario M, Guerrero SInt J Food Microbiol10.1016/j.ijfoodmicro.2022.1100242022
36462814Using regression and Multifactorial Analysis of Variance to assess the effect of ascorbic, citric, and malic acids on spores and activated spores of Alicyclobacillusacidoterrestris.Bevilacqua A, Speranza B, Petruzzi L, Sinigaglia M, Corbo MRFood Microbiol10.1016/j.fm.2022.1041582022*Malates/pharmacology, *Citric Acid, Spores, Ascorbic Acid/pharmacology, Analysis of Variance

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1510Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3922)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3922
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41589Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18229
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
70080Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1091.1StrainInfo: A central database for resolving microbial strain identifiers
116022Curators of the CIPCollection of Institut Pasteur (CIP 106132)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106132