Strain identifier

BacDive ID: 3988

Type strain: Yes

Species: Desulfofrigus oceanense

Strain Designation: ASv26

Strain history: <- C. Knoblauch, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; ASv26 <- C. Knoblauch {1997}

NCBI tax ID(s): 84982 (species)

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General

@ref: 4702

BacDive-ID: 3988

DSM-Number: 12341

keywords: 16S sequence, Bacteria, anaerobe, chemoorganotroph, psychrophilic, Gram-negative

description: Desulfofrigus oceanense ASv26 is an anaerobe, chemoorganotroph, psychrophilic bacterium that was isolated from marine mud.

NCBI tax id

  • NCBI tax id: 84982
  • Matching level: species

strain history

  • @ref: 4702
  • history: <- C. Knoblauch, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; ASv26 <- C. Knoblauch {1997}

doi: 10.13145/bacdive3988.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Desulfobacterales
  • family: Desulfobacteraceae
  • genus: Desulfofrigus
  • species: Desulfofrigus oceanense
  • full scientific name: Desulfofrigus oceanense Knoblauch et al. 1999

@ref: 4702

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Desulfobacterales

family: Desulfobacteraceae

genus: Desulfofrigus

species: Desulfofrigus oceanense

full scientific name: Desulfofrigus oceanense Knoblauch et al. 1999

strain designation: ASv26

type strain: yes

Morphology

cell morphology

  • @ref: 23372
  • gram stain: negative
  • cell length: 4.2-6.1 µm
  • cell width: 2.1 µm

colony morphology

  • @ref: 4702
  • incubation period: >14 days

Culture and growth conditions

culture medium

  • @ref: 4702
  • name: DESULFOFRIGUS MEDIUM (DSMZ Medium 861)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/861
  • composition: Name: DESULFOFRIGUS MEDIUM (DSMZ Medium 861; with strain-specific modifications) Composition: NaCl 19.9203 g/l Na2SO4 3.98406 g/l MgCl2 x 6 H2O 2.98805 g/l Na-acetate 1.49402 g/l Na2CO3 1.49402 g/l KCl 0.498008 g/l Na2S x 9 H2O 0.298805 g/l NH4Cl 0.249004 g/l KH2PO4 0.199203 g/l CaCl2 x 2 H2O 0.149402 g/l KBr 0.0896414 g/l Na2S2O4 0.0498008 g/l NaHCO3 0.0498008 g/l HCl 0.00249004 g/l FeCl2 x 4 H2O 0.00149402 g/l NaOH 0.000498008 g/l Sodium resazurin 0.000498008 g/l CoCl2 x 6 H2O 0.000189243 g/l Pyridoxine hydrochloride 9.96016e-05 g/l MnCl2 x 4 H2O 9.96016e-05 g/l ZnCl2 6.97211e-05 g/l p-Aminobenzoic acid 4.98008e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l Nicotinic acid 4.98008e-05 g/l Riboflavin 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Na2MoO4 x 2 H2O 3.58566e-05 g/l NiCl2 x 6 H2O 2.39044e-05 g/l Folic acid 1.99203e-05 g/l Biotin 1.99203e-05 g/l H3BO3 5.9761e-06 g/l Na2WO4 x 2 H2O 3.98406e-06 g/l Na2SeO3 x 5 H2O 2.98805e-06 g/l CuCl2 x 2 H2O 1.99203e-06 g/l Vitamin B12 9.96016e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4702positivegrowth8psychrophilic
23372positivegrowth-1.8-16psychrophilic
23372positiveoptimum10psychrophilic

culture pH

  • @ref: 23372
  • ability: positive
  • type: optimum
  • pH: 7.0-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4702anaerobe
23372obligate anaerobe

nutrition type

  • @ref: 23372
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentration
23372NaClpositiveoptimum1.5-2.5 %
23372MgCl2positivegrowth0.003-2.0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2337233403elemental sulfur-electron acceptor
2337216449alanine-electron donor
2337227689decanoate-electron donor
2337229806fumarate-electron donor
233727896hexadecanoate-electron donor
2337217272propionate-electron donor
2337230031succinate-electron donor
2337229806fumarate-fermentation
2337233403elemental sulfur-reduction
2337217632nitrate-reduction
2337216301nitrite-reduction
2337230089acetate+carbon source
2337228885butanol+carbon source
2337217968butyrate+carbon source
2337216236ethanol+carbon source
2337215740formate+carbon source
2337217754glycerol+carbon source
2337215428glycine+carbon source
2337224996lactate+carbon source
2337225115malate+carbon source
2337228831propanol+carbon source
2337215361pyruvate+carbon source
2337217822serine+carbon source
2337231011valerate+carbon source
2337216189sulfate+electron acceptor
2337217359sulfite+electron acceptor
2337216094thiosulfate+electron acceptor
2337230089acetate+electron donor
2337228885butanol+electron donor
2337217968butyrate+electron donor
2337216236ethanol+electron donor
2337215740formate+electron donor
2337224996lactate+electron donor
2337225115malate+electron donor
2337228831propanol+electron donor
2337215361pyruvate+electron donor
2337231011valerate+electron donor
2337224996lactate+fermentation
2337225115malate+fermentation
2337215361pyruvate+fermentation
2337217754glycerol+/-electron donor
2337215428glycine+/-electron donor
2337217822serine+/-electron donor
2337233403elemental sulfurelectron acceptor
2337216189sulfateelectron acceptor
2337217359sulfiteelectron acceptor
2337216094thiosulfateelectron acceptor
2337216449alanineelectron donor
2337228885butanolelectron donor
2337217968butyrateelectron donor
2337227689decanoateelectron donor
2337216236ethanolelectron donor
2337229806fumarateelectron donor
2337217754glycerolelectron donor
2337215428glycineelectron donor
233727896hexadecanoateelectron donor
2337224996lactateelectron donor
2337225115malateelectron donor
2337228831propanolelectron donor
2337217272propionateelectron donor
2337215361pyruvateelectron donor
2337217822serineelectron donor
2337232954sodium acetateelectron donor
2337230031succinateelectron donor
2337231011valerateelectron donor
2337229806fumaratefermentation
2337224996lactatefermentation
2337225115malatefermentation
2337215361pyruvatefermentation

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4702marine mudSpitsbergen, HornsundNorwayNOREurope
23372permanently cold marine sediments

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment
#Condition#Psychrophilic (<10°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_56159.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_705;96_25002;97_31330;98_40098;99_56159&stattab=map
  • Last taxonomy: Desulfofrigus oceanense subclade
  • 16S sequence: AF099064
  • Sequence Identity:
  • Total samples: 96
  • soil counts: 8
  • aquatic counts: 88

Safety information

risk assessment

  • @ref: 4702
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4702
  • description: Desulfofrigus oceanense strain ASv26 16S ribosomal RNA gene, partial sequence
  • accession: AF099064
  • length: 1523
  • database: ena
  • NCBI tax ID: 84982

GC content

  • @ref: 4702
  • GC-content: 52.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4702

culture collection no.: DSM 12341

straininfo link

  • @ref: 73494
  • straininfo: 49271

literature

  • topic: Phylogeny
  • Pubmed-ID: 10555345
  • title: Psychrophilic sulfate-reducing bacteria isolated from permanently cold arctic marine sediments: description of Desulfofrigus oceanense gen. nov., sp. nov., Desulfofrigus fragile sp. nov., Desulfofaba gelida gen. nov., sp. nov., Desulfotalea psychrophila gen. nov., sp. nov. and Desulfotalea arctica sp. nov.
  • authors: Knoblauch C, Sahm K, Jorgensen BB
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-4-1631
  • year: 1999
  • mesh: Arctic Regions, Bacterial Typing Techniques, Base Composition, Cold Temperature, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Deltaproteobacteria/*classification/isolation & purification, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sulfur-Reducing Bacteria/*classification/cytology/isolation & purification/physiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4702Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12341)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12341
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23372Christian Knoblauch, Kerstin Sahm, Bo B. Jorgensen10.1099/00207713-49-4-1631Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigus oceanense gen. nov., sp. nov., Desulfofrigus fragile sp. nov., Desulfofaba gelida gen. nov., sp. nov., Desulfotalea psychrophila gen. nov., sp. nov. and Desulfotalea arctica sp. nov.IJSEM 49: 1631-1643 199910555345
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73494Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49271.1StrainInfo: A central database for resolving microbial strain identifiers