Strain identifier

BacDive ID: 395

Type strain: Yes

Species: Alcanivorax balearicus

Strain Designation: MACL04

Strain history: CIP <- 2007, CECT <- 2004, E. Velazquez, Salamanca Univ., Salamanca, Spain: strain MACL04

NCBI tax ID(s): 413232 (species)

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General

@ref: 17370

BacDive-ID: 395

DSM-Number: 23776

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Alcanivorax balearicus MACL04 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from water from a subterranean saline lake.

NCBI tax id

  • NCBI tax id: 413232
  • Matching level: species

strain history

@refhistory
17370<- CECT <- E. Velázquez <- R. Rivas, Depart. Microbiol. y Gen., Univ. Salamanca, Spain
116715CIP <- 2007, CECT <- 2004, E. Velazquez, Salamanca Univ., Salamanca, Spain: strain MACL04

doi: 10.13145/bacdive395.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Alcanivoracaceae
  • genus: Alcanivorax
  • species: Alcanivorax balearicus
  • full scientific name: Alcanivorax balearicus Rivas et al. 2007

  • synonyms

    • @ref: 20215
    • synonym: Alloalcanivorax balearicus

@ref: 17370

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Alcanivoracaceae

genus: Alcanivorax

species: Alcanivorax balearicus

full scientific name: Alcanivorax balearicus Rivas et al. 2007

strain designation: MACL04

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
32069negativerod-shapedyes
116715negativerod-shapedyes
125438negative98.5

colony morphology

  • @ref: 17370
  • incubation period: 2-3 days

pigmentation

  • @ref: 32069
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17370ALCANIVORAX BALERICUS MEDIUM (DSMZ Medium 1289)yeshttps://mediadive.dsmz.de/medium/1289Name: ALCANIVORAX BALERICUS MEDIUM (DSMZ Medium 1289) Composition: Agar 15.0 g/l Peptone 10.0 g/l NaCl 5.0 g/l Beef extract 5.0 g/l Distilled water
37823MEDIUM 666 - for AlcanivoraxyesDistilled water make up to (1.000 l);Sodium chloride (30.000 g);Agar (15.000 g);Yeast extract (5.000 g);Tryptone (10.000 g)
116715CIP Medium 325yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=325

culture temp

@refgrowthtypetemperature
17370positivegrowth28
32069positivegrowth04-37
32069positiveoptimum28
37823positivegrowth30

culture pH

@refabilitytypepHPH range
32069positivegrowth5.5-9alkaliphile
32069positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32069aerobe
116715obligate aerobe

spore formation

  • @ref: 125438
  • spore formation: no
  • confidence: 94.29

halophily

@refsaltgrowthtested relationconcentration
32069NaClpositivegrowth0-15 %
32069NaClpositiveoptimum7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3206930089acetate+carbon source
3206922599arabinose+carbon source
3206916947citrate+carbon source
3206917234glucose+carbon source
3206925115malate+carbon source
3206929864mannitol+carbon source
3206918401phenylacetate+carbon source
3206917272propionate+carbon source
3206915361pyruvate+carbon source
11671517632nitrate+reduction
11671516301nitrite+reduction

metabolite production

  • @ref: 116715
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32069catalase+1.11.1.6
32069cytochrome oxidase+1.9.3.1
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116715oxidase+
116715catalase+1.11.1.6
116715urease-3.5.1.5
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382naphthol-AS-BI-phosphohydrolase+
68382lipase (C 14)+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116715-+++++----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17370water from a subterranean saline lakeBalearic Islands, Palma de Mallorca, Lake MartelSpainESPEurope
116715Environment, Lake Martel, saline lakePalma de MallorcaSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_740.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15837;96_450;97_516;98_593;99_740&stattab=map
  • Last taxonomy: Alcanivorax
  • 16S sequence: AY686709
  • Sequence Identity:
  • Total samples: 10696
  • soil counts: 778
  • aquatic counts: 8225
  • animal counts: 1456
  • plant counts: 237

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
173701Risk group (German classification)
1167151Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17370
  • description: Alcanivorax balearicus strain MACL04 16S ribosomal RNA gene, partial sequence
  • accession: AY686709
  • length: 1540
  • database: nuccore
  • NCBI tax ID: 1177182

Genome sequences

  • @ref: 66792
  • description: Alloalcanivorax balearicus MACL04
  • accession: GCA_025532145
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1177182

GC content

@refGC-contentmethod
1737062.8thermal denaturation, midpoint method (Tm)
3206962.8

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno98.5no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.866yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes83.643no
125438spore-formingspore-formingAbility to form endo- or exosporesno94.29no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno97.5no
125438motile2+flagellatedAbility to perform flagellated movementyes87.76no

External links

@ref: 17370

culture collection no.: DSM 23776, CECT 5683, LMG 22508, CIP 109716

straininfo link

  • @ref: 70075
  • straininfo: 131631

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17551053Alcanivorax balearicus sp. nov., isolated from Lake Martel.Rivas R, Garcia-Fraile P, Peix A, Mateos PF, Martinez-Molina E, Velazquez EInt J Syst Evol Microbiol10.1099/ijs.0.64912-02007Alcanivoraceae/*classification/drug effects/*isolation & purification/physiology, Alkanes/metabolism, Amino Acids/metabolism, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, Carboxylic Acids/metabolism, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Fatty Acids/analysis, Flagella/physiology, Genes, rRNA, Lipase/analysis, Locomotion, Mixed Function Oxygenases/genetics, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic/pharmacology, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Spain, *Water MicrobiologyMetabolism
Phylogeny25061066Alcanivorax xenomutans sp. nov., a hydrocarbonoclastic bacterium isolated from a shrimp cultivation pond.Rahul K, Sasikala C, Tushar L, Debadrita R, Ramana CVInt J Syst Evol Microbiol10.1099/ijs.0.061168-02014Alcanivoraceae/*classification/genetics/isolation & purification, Animals, Aquaculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Decapoda, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17370Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23776)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23776
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32069Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2831628776041
37823Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7490
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70075Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID131631.1StrainInfo: A central database for resolving microbial strain identifiers
116715Curators of the CIPCollection of Institut Pasteur (CIP 109716)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109716
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets