Strain identifier
BacDive ID: 3942
Type strain:
Species: Austwickia chelonae
Strain Designation: W16, W 16
Strain history: CIP <- 1995, A. Masters, Animal Hlth. Lab., Dept. Agri. and food Western Australia, South Perth, Western Australia: strain W 16 <- Dept. Agri. and food, Western Australia, Australia
NCBI tax ID(s): 1184607 (strain), 100225 (species)
General
@ref: 11576
BacDive-ID: 3942
DSM-Number: 44178
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, animal pathogen
description: Austwickia chelonae W16 is an aerobe, mesophilic animal pathogen that was isolated from snapping turtle .
NCBI tax id
NCBI tax id | Matching level |
---|---|
100225 | species |
1184607 | strain |
strain history
@ref | history |
---|---|
11576 | <- CIP <- A. Masters, W 16 (1995), Animal Health Lab., Dept. Agri Western <- Agricult. Dept. Western Australia |
67770 | CIP 104541 <-- A. M. Masters W16. |
118227 | CIP <- 1995, A. Masters, Animal Hlth. Lab., Dept. Agri. and food Western Australia, South Perth, Western Australia: strain W 16 <- Dept. Agri. and food, Western Australia, Australia |
doi: 10.13145/bacdive3942.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Dermatophilaceae
- genus: Austwickia
- species: Austwickia chelonae
- full scientific name: Austwickia chelonae (Masters et al. 1995) Hamada et al. 2011
synonyms
- @ref: 20215
- synonym: Dermatophilus chelonae
@ref: 11576
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Dermatophilaceae
genus: Austwickia
species: Austwickia chelonae
full scientific name: Austwickia chelonae (Masters et al. 1995) Hamada et al. 2011
strain designation: W16, W 16
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19847 | Ivory | 10-14 days | ISP 2 |
19847 | Ivory | 10-14 days | ISP 3 |
19847 | Ivory | 10-14 days | ISP 4 |
19847 | 10-14 days | ISP 5 | |
19847 | Ivory | 10-14 days | ISP 6 |
19847 | 10-14 days | ISP 7 | |
57694 | 3 days |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19847 | no | ISP 2 |
19847 | no | ISP 3 |
19847 | no | ISP 4 |
19847 | no | ISP 5 |
19847 | no | ISP 6 |
19847 | no | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11576 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535; with strain-specific modifications) Composition: Defibrinated sheep blood 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
19847 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19847 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19847 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19847 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19847 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19847 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
40148 | MEDIUM 6 - Columbia agar with 10 % horse blood | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml) | |
118227 | CIP Medium 6 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11576 | positive | growth | 37 | mesophilic |
40148 | positive | growth | 30 | mesophilic |
57694 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 57694
- oxygen tolerance: aerobe
halophily
- @ref: 19847
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 7.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68368 | 5291 | gelatin | + | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | + | hydrolysis |
68368 | 16947 | citrate | + | assimilation |
68368 | 18257 | ornithine | + | degradation |
68368 | 25094 | lysine | + | degradation |
68368 | 29016 | arginine | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | + | 3.5.1.5 |
68368 | ornithine decarboxylase | + | 4.1.1.17 |
68368 | lysine decarboxylase | + | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19847 | + | + | + | - | + | + | + | + | - | + | - | - | - | - | + | + | - | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
---|---|---|---|---|---|---|---|---|---|---|---|
19847 | - | + | + | + | + | - | + | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date | isolation date |
---|---|---|---|---|---|---|---|
11576 | snapping turtle (nose scab) | Perth Western | Australia | AUS | Australia and Oceania | ||
57694 | Snapping turtle,nose scab | Perth | Australia | AUS | Australia and Oceania | 1987 | |
67770 | Nose scab on a snapping turtle | Perth Zoo, Western Australia | Australia | AUS | Australia and Oceania | ||
118227 | Snapping turtle, nose scab | Perth | Australia | AUS | Australia and Oceania | 1987 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Reptilia | |
#Host Body-Site | #Other | #Wound |
#Host Body-Site | #Organ | #Nose |
taxonmaps
- @ref: 69479
- File name: preview.99_4905.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2413;97_2938;98_3672;99_4905&stattab=map
- Last taxonomy: Austwickia chelonae subclade
- 16S sequence: AJ243919
- Sequence Identity:
- Total samples: 57
- soil counts: 10
- aquatic counts: 6
- animal counts: 40
- plant counts: 1
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
11576 | yes | 2 | Risk group (German classification) |
19847 | 2 | Hazard group | |
118227 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Dermatophilus chelonae gene for 16S rRNA, partial sequence, strain: NBRC 105200 | AB550801 | 1475 | ena | 1184607 |
11576 | Dermatophilus chelonae partial 16S rRNA gene, DSM 44178 type strain | AJ243919 | 1495 | ena | 1184607 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Austwickia chelonae NBRC 105200 | 1184607.3 | wgs | patric | 1184607 |
66792 | Austwickia chelonae strain DSM 44178 | 100225.5 | wgs | patric | 100225 |
66792 | Austwickia chelonae DSM 44178 | 2687453746 | draft | img | 100225 |
66792 | Austwickia chelonae NBRC 105200 | 2537562210 | draft | img | 1184607 |
67770 | Austwickia chelonae NBRC 105200 | GCA_000298175 | contig | ncbi | 1184607 |
67770 | Austwickia chelonae DSM 44178 | GCA_900111385 | contig | ncbi | 100225 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 51.951 | no |
gram-positive | yes | 84.853 | no |
anaerobic | no | 90.348 | no |
aerobic | no | 51.385 | no |
halophile | no | 96.019 | no |
spore-forming | no | 76.506 | no |
flagellated | no | 89.622 | no |
thermophile | no | 87.809 | yes |
glucose-util | yes | 86.751 | no |
glucose-ferment | no | 63.373 | no |
External links
@ref: 11576
culture collection no.: DSM 44178, ATCC 51576, CCUG 47447, CIP 104541, JCM 9706, NBRC 105200
straininfo link
- @ref: 73451
- straininfo: 45303
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 7857807 | Dermatophilus chelonae sp. nov., isolated from chelonids in Australia. | Masters AM, Ellis TM, Carson JM, Sutherland SS, Gregory AR | Int J Syst Bacteriol | 10.1099/00207713-45-1-50 | 1995 | Actinomycetales/growth & development/*isolation & purification/metabolism, Animals, Australia, Turtles/*microbiology | Enzymology |
Phylogeny | 21282898 | Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov. | Hamada M, Iino T, Iwami T, Harayama S, Tamura T, Suzuki K | J Gen Appl Microbiol | 10.2323/jgam.56.427 | 2010 | Actinomycetales/*classification/genetics/isolation & purification/physiology, Animals, *Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Fishes/microbiology, Intestines/microbiology, Japan, Microscopy, Electron, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Vitamin K 2/analysis | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11576 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44178) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44178 | |||
19847 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44178.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40148 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16461 | ||||
57694 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 47447) | https://www.ccug.se/strain?id=47447 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
73451 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45303.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118227 | Curators of the CIP | Collection of Institut Pasteur (CIP 104541) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104541 |