Strain identifier

BacDive ID: 3942

Type strain: Yes

Species: Austwickia chelonae

Strain Designation: W16, W 16

Strain history: CIP <- 1995, A. Masters, Animal Hlth. Lab., Dept. Agri. and food Western Australia, South Perth, Western Australia: strain W 16 <- Dept. Agri. and food, Western Australia, Australia

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General

@ref: 11576

BacDive-ID: 3942

DSM-Number: 44178

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, animal pathogen

description: Austwickia chelonae W16 is an aerobe, mesophilic animal pathogen that was isolated from snapping turtle .

NCBI tax id

NCBI tax idMatching level
100225species
1184607strain

strain history

@refhistory
11576<- CIP <- A. Masters, W 16 (1995), Animal Health Lab., Dept. Agri Western <- Agricult. Dept. Western Australia
67770CIP 104541 <-- A. M. Masters W16.
118227CIP <- 1995, A. Masters, Animal Hlth. Lab., Dept. Agri. and food Western Australia, South Perth, Western Australia: strain W 16 <- Dept. Agri. and food, Western Australia, Australia

doi: 10.13145/bacdive3942.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Dermatophilaceae
  • genus: Austwickia
  • species: Austwickia chelonae
  • full scientific name: Austwickia chelonae (Masters et al. 1995) Hamada et al. 2011
  • synonyms

    • @ref: 20215
    • synonym: Dermatophilus chelonae

@ref: 11576

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Dermatophilaceae

genus: Austwickia

species: Austwickia chelonae

full scientific name: Austwickia chelonae (Masters et al. 1995) Hamada et al. 2011

strain designation: W16, W 16

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19847Ivory10-14 daysISP 2
19847Ivory10-14 daysISP 3
19847Ivory10-14 daysISP 4
1984710-14 daysISP 5
19847Ivory10-14 daysISP 6
1984710-14 daysISP 7
576943 days

multicellular morphology

@refforms multicellular complexmedium name
19847noISP 2
19847noISP 3
19847noISP 4
19847noISP 5
19847noISP 6
19847noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11576TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535; with strain-specific modifications) Composition: Defibrinated sheep blood 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19847ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19847ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19847ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19847ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19847ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19847ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40148MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
118227CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
11576positivegrowth37mesophilic
40148positivegrowth30mesophilic
57694positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 57694
  • oxygen tolerance: aerobe

halophily

  • @ref: 19847
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19847+++-++++-+----++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19847-++++-+---+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
11576snapping turtle (nose scab)Perth WesternAustraliaAUSAustralia and Oceania
57694Snapping turtle,nose scabPerthAustraliaAUSAustralia and Oceania1987
67770Nose scab on a snapping turtlePerth Zoo, Western AustraliaAustraliaAUSAustralia and Oceania
118227Snapping turtle, nose scabPerthAustraliaAUSAustralia and Oceania1987

isolation source categories

Cat1Cat2Cat3
#Host#Reptilia
#Host Body-Site#Other#Wound
#Host Body-Site#Organ#Nose

taxonmaps

  • @ref: 69479
  • File name: preview.99_4905.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2413;97_2938;98_3672;99_4905&stattab=map
  • Last taxonomy: Austwickia chelonae subclade
  • 16S sequence: AJ243919
  • Sequence Identity:
  • Total samples: 57
  • soil counts: 10
  • aquatic counts: 6
  • animal counts: 40
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
11576yes2Risk group (German classification)
198472Hazard group
1182271Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Dermatophilus chelonae gene for 16S rRNA, partial sequence, strain: NBRC 105200AB5508011475ena1184607
11576Dermatophilus chelonae partial 16S rRNA gene, DSM 44178 type strainAJ2439191495ena1184607

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Austwickia chelonae NBRC 1052001184607.3wgspatric1184607
66792Austwickia chelonae strain DSM 44178100225.5wgspatric100225
66792Austwickia chelonae DSM 441782687453746draftimg100225
66792Austwickia chelonae NBRC 1052002537562210draftimg1184607
67770Austwickia chelonae NBRC 105200GCA_000298175contigncbi1184607
67770Austwickia chelonae DSM 44178GCA_900111385contigncbi100225

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes51.951no
gram-positiveyes84.853no
anaerobicno90.348no
aerobicno51.385no
halophileno96.019no
spore-formingno76.506no
flagellatedno89.622no
thermophileno87.809yes
glucose-utilyes86.751no
glucose-fermentno63.373no

External links

@ref: 11576

culture collection no.: DSM 44178, ATCC 51576, CCUG 47447, CIP 104541, JCM 9706, NBRC 105200

straininfo link

  • @ref: 73451
  • straininfo: 45303

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7857807Dermatophilus chelonae sp. nov., isolated from chelonids in Australia.Masters AM, Ellis TM, Carson JM, Sutherland SS, Gregory ARInt J Syst Bacteriol10.1099/00207713-45-1-501995Actinomycetales/growth & development/*isolation & purification/metabolism, Animals, Australia, Turtles/*microbiologyEnzymology
Phylogeny21282898Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.Hamada M, Iino T, Iwami T, Harayama S, Tamura T, Suzuki KJ Gen Appl Microbiol10.2323/jgam.56.4272010Actinomycetales/*classification/genetics/isolation & purification/physiology, Animals, *Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Fishes/microbiology, Intestines/microbiology, Japan, Microscopy, Electron, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11576Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44178)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44178
19847Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44178.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40148Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16461
57694Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47447)https://www.ccug.se/strain?id=47447
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73451Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45303.1StrainInfo: A central database for resolving microbial strain identifiers
118227Curators of the CIPCollection of Institut Pasteur (CIP 104541)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104541