Strain identifier

BacDive ID: 394

Type strain: Yes

Species: Alcanivorax dieselolei

Strain Designation: B-5

Strain history: <- Z. Shao, State Oceanic Admin., Third Inst. Oceanography, China; B-5 <- C. Liu

NCBI tax ID(s): 285091 (species)

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General

@ref: 6379

BacDive-ID: 394

DSM-Number: 16502

keywords: genome sequence, 16S sequence, Bacteria, aerobe, halophilic, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Alcanivorax dieselolei B-5 is an aerobe, halophilic, mesophilic bacterium that forms irregular colonies and was isolated from oil-contaminated sea water at the Yellow River dock of Shengli oilfield.

NCBI tax id

  • NCBI tax id: 285091
  • Matching level: species

strain history

  • @ref: 6379
  • history: <- Z. Shao, State Oceanic Admin., Third Inst. Oceanography, China; B-5 <- C. Liu

doi: 10.13145/bacdive394.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Alcanivoracaceae
  • genus: Alcanivorax
  • species: Alcanivorax dieselolei
  • full scientific name: Alcanivorax dieselolei Liu and Shao 2005

  • synonyms

    • @ref: 20215
    • synonym: Alloalcanivorax dieselolei

@ref: 6379

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Alcanivoracaceae

genus: Alcanivorax

species: Alcanivorax dieselolei

full scientific name: Alcanivorax dieselolei Liu and Shao 2005

strain designation: B-5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23284negative0.8-2.0 µm0.3-0.7 µmrod-shapedyeslophotrichous
69480yes92.822
69480negative99.999

colony morphology

  • @ref: 23284
  • colony color: translucent
  • colony shape: irregular
  • medium used: HLB agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6379BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l Na-pyruvate 10.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23284HLB agaryes

culture temp

@refgrowthtypetemperaturerange
6379positivegrowth28mesophilic
23284positivegrowth15-45
23284nogrowth4psychrophilic
23284positivegrowth45thermophilic
23284positiveoptimum28mesophilic
23284mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 23284
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
23284no
69481no100
69480no99.995

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23284halophilic
23284NaClpositivegrowth1-15 %
23284NaClpositiveoptimum3-7.5 %
23284NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2328415963ribitol-assimilation
2328436219alpha-lactose-assimilation
2328417057cellobiose-assimilation
2328418333D-arabitol-assimilation
2328415824D-fructose-assimilation
2328412936D-galactose-assimilation
2328417634D-glucose-assimilation
2328416899D-mannitol-assimilation
2328416024D-mannose-assimilation
2328428053melibiose-assimilation
2328416634raffinose-assimilation
2328417924D-sorbitol-assimilation
2328416551D-trehalose-assimilation
2328417113erythritol-assimilation
23284167244-hydroxybutyrate-assimilation
2328428066gentiobiose-assimilation
2328430849L-arabinose-assimilation
2328418287L-fucose-assimilation
2328462345L-rhamnose-assimilation
232846359lactulose-assimilation
2328417306maltose-assimilation
23284320055methyl beta-D-glucopyranoside-assimilation
2328417268myo-inositol-assimilation
2328428037N-acetylgalactosamine-assimilation
23284506227N-acetylglucosamine-assimilation
2328433951psicose-assimilation
2328417992sucrose-assimilation
2328432528turanose-assimilation
2328417151xylitol-assimilation
23284620642,3-butanediol+assimilation
23284181014-hydroxyphenylacetic acid+assimilation
2328430089acetate+assimilation
232848295beta-hydroxybutyrate+assimilation
2328416947citrate+assimilation
2328424996lactate+assimilation
2328451850methyl pyruvate+assimilation
2328475146monomethyl succinate+assimilation
2328417272propionate+assimilation
2328430031succinate+assimilation
2328453423tween 40+assimilation
2328453426tween 80+assimilation
23284181014-hydroxyphenylacetic acid+carbon source
2328416947citrate+carbon source
2328424996lactate+carbon source
2328415361pyruvate+carbon source
2328441808decane+degradation
2328446050docosane+degradation
2328428817dodecane+degradation
2328436020dotriacontane+degradation
2328432931henicosane+degradation
232845659hentriacontane+degradation
2328432941heptacosane+degradation
2328416148heptadecane+degradation
2328443098heptane+degradation
2328432940hexacosane+degradation
2328445296hexadecane+degradation
2328429021hexane+degradation
2328472688hexatriacontane+degradation
2328443619icosane+degradation
232847613nonacosane+degradation
2328432927nonadecane+degradation
2328432892nonane+degradation
2328432943octacosane+degradation
2328432926octadecane+degradation
2328417590octane+degradation
2328432938pentacosane+degradation
2328428897pentadecane+degradation
2328437830pentane+degradation
23284pentatriacontane+degradation
2328432936tetracosane+degradation
2328441253tetradecane+degradation
2328487443tetratriacontane+degradation
2328431006triacontane+degradation
2328432934tricosane+degradation
2328435998tridecane+degradation
232849751tritriacontane+degradation
2328453426tween 80+degradation
2328446342undecane+degradation
2328417632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitive
232842637amikacinyesyes
232846104kanamycinyesyes
232847507neomycinyesyes
232848309polymyxin byesyes

enzymes

@refvalueactivityec
23284agarase-3.2.1.81
23284amylase-
23284arginine dihydrolase-3.5.3.6
23284catalase+1.11.1.6
23284cytochrome oxidase+1.9.3.1
23284gelatinase-

Isolation, sampling and environmental information

isolation

  • @ref: 6379
  • sample type: oil-contaminated sea water at the Yellow River dock of Shengli oilfield
  • geographic location: Bohai Sea
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination#Oil (Fuel)
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_740.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15837;96_450;97_516;98_593;99_740&stattab=map
  • Last taxonomy: Alcanivorax
  • 16S sequence: AY683537
  • Sequence Identity:
  • Total samples: 10696
  • soil counts: 778
  • aquatic counts: 8225
  • animal counts: 1456
  • plant counts: 237

Safety information

risk assessment

  • @ref: 6379
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6379
  • description: Alcanivorax dieselolei strain B-5 clone 1 16S ribosomal RNA gene, partial sequence
  • accession: AY683537
  • length: 1504
  • database: ena
  • NCBI tax ID: 285091

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alloalcanivorax dieselolei CGMCC 1.3690GCA_014644575contigncbi285091
66792Alcanivorax dieselolei strain CGMCC 1.3690285091.9wgspatric285091

GC content

@refGC-contentmethod
637962.1
2328462.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes92.806yes
gram-positiveno98.675no
anaerobicno98.461yes
halophileyes82.777no
spore-formingno96.344yes
glucose-utilno76.211yes
flagellatedyes87.332yes
aerobicyes95.529yes
thermophileno99.377no
glucose-fermentno88.379no

External links

@ref: 6379

culture collection no.: DSM 16502, CGMCC 1.3690

straininfo link

  • @ref: 70074
  • straininfo: 138966

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15879252Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment.Liu C, Shao ZInt J Syst Evol Microbiol10.1099/ijs.0.63443-02005Aerobiosis, Alkanes/*metabolism, Base Composition, Carboxylic Acids/metabolism, Catalase/analysis, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, DNA, Ribosomal Spacer/genetics, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Geologic Sediments/*microbiology, Growth Inhibitors/pharmacology, Halomonadaceae/*classification/cytology/*isolation & purification/metabolism, Hydrolases/genetics, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Oxidoreductases/analysis, Pacific Ocean, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sodium Chloride/pharmacology, Water MicrobiologyEnzymology
Phylogeny25061066Alcanivorax xenomutans sp. nov., a hydrocarbonoclastic bacterium isolated from a shrimp cultivation pond.Rahul K, Sasikala C, Tushar L, Debadrita R, Ramana CVInt J Syst Evol Microbiol10.1099/ijs.0.061168-02014Alcanivoraceae/*classification/genetics/isolation & purification, Animals, Aquaculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Decapoda, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spain, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6379Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16502)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16502
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23284Chenli Liu, Zongze Shao10.1099/ijs.0.63443-0Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sedimentIJSEM 55: 1181-1186 200515879252
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70074Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID138966.1StrainInfo: A central database for resolving microbial strain identifiers