Strain identifier
BacDive ID: 3915
Type strain: 
Species: Brachybacterium phenoliresistens
Strain Designation: phenol-A
Strain history: BCRC 17589 <-- W.-M. Chen strain phenol-A.
NCBI tax ID(s): 396014 (species)
version 8.1 (current version)
General
@ref: 16262
BacDive-ID: 3915
DSM-Number: 22299
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, ovoid-shaped
description: Brachybacterium phenoliresistens phenol-A is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from oil-contaminated coastal sand.
NCBI tax id
- NCBI tax id: 396014
- Matching level: species
strain history
| @ref | history |
|---|---|
| 16262 | <- LMG <- W.-M. Chen, Natl. Kaohsiung Marine Univ. Taiwan; phenol-A |
| 67770 | BCRC 17589 <-- W.-M. Chen strain phenol-A. |
doi: 10.13145/bacdive3915.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Dermabacteraceae
- genus: Brachybacterium
- species: Brachybacterium phenoliresistens
- full scientific name: Brachybacterium phenoliresistens Chou et al. 2007
@ref: 16262
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Dermabacteraceae
genus: Brachybacterium
species: Brachybacterium phenoliresistens
full scientific name: Brachybacterium phenoliresistens Chou et al. 2007
strain designation: phenol-A
type strain: yes
Morphology
cell morphology
- @ref: 32133
- gram stain: positive
- cell length: 1.3 µm
- cell width: 0.8 µm
- cell shape: ovoid-shaped
- motility: no
pigmentation
- @ref: 32133
- production: yes
Culture and growth conditions
culture medium
- @ref: 16262
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16262 | positive | growth | 28 | mesophilic |
| 32133 | positive | growth | 04-40 | |
| 32133 | positive | optimum | 32.5 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 32133 | positive | growth | 05-10 | alkaliphile |
| 32133 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 32133
- oxygen tolerance: facultative anaerobe
spore formation
- @ref: 32133
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 32133 | NaCl | positive | growth | 0.5-7 % |
| 32133 | NaCl | positive | optimum | 2.5 % |
murein
- @ref: 16262
- murein short key: A31.03
- type: A4gamma m-Dpm-D-Asp-D-Glu; alpha-carboxyl group of D-Glu substituted by glycine
observation
| @ref | observation |
|---|---|
| 32133 | aggregates in clumps |
| 67770 | quinones: MK-7 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 32133 | 22599 | arabinose | + | carbon source |
| 32133 | 18403 | L-arabitol | + | carbon source |
| 32133 | 35391 | aspartate | + | carbon source |
| 32133 | 17057 | cellobiose | + | carbon source |
| 32133 | 23652 | dextrin | + | carbon source |
| 32133 | 28757 | fructose | + | carbon source |
| 32133 | 28260 | galactose | + | carbon source |
| 32133 | 17234 | glucose | + | carbon source |
| 32133 | 15428 | glycine | + | carbon source |
| 32133 | 28087 | glycogen | + | carbon source |
| 32133 | 17716 | lactose | + | carbon source |
| 32133 | 17306 | maltose | + | carbon source |
| 32133 | 29864 | mannitol | + | carbon source |
| 32133 | 37684 | mannose | + | carbon source |
| 32133 | 506227 | N-acetylglucosamine | + | carbon source |
| 32133 | 51850 | methyl pyruvate | + | carbon source |
| 32133 | 16634 | raffinose | + | carbon source |
| 32133 | 26546 | rhamnose | + | carbon source |
| 32133 | 17814 | salicin | + | carbon source |
| 32133 | 30911 | sorbitol | + | carbon source |
| 32133 | 17992 | sucrose | + | carbon source |
| 32133 | 27082 | trehalose | + | carbon source |
| 32133 | 53424 | tween 20 | + | carbon source |
| 32133 | 53423 | tween 40 | + | carbon source |
| 32133 | 18222 | xylose | + | carbon source |
| 32133 | 4853 | esculin | + | hydrolysis |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 32133 | acid phosphatase | + | 3.1.3.2 |
| 32133 | alkaline phosphatase | + | 3.1.3.1 |
| 32133 | catalase | + | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 16262 | oil-contaminated coastal sand | Pingtung County | Taiwan | TWN | Asia |
| 67770 | Oil-contaminated coastal sand | Pingtung County | Taiwan | TWN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | #Oil (Fuel) |
| #Environmental | #Terrestrial | #Coast |
| #Environmental | #Terrestrial | #Sandy |
taxonmaps
- @ref: 69479
- File name: preview.99_6192.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1217;97_3626;98_4590;99_6192&stattab=map
- Last taxonomy: Brachybacterium phenoliresistens subclade
- 16S sequence: DQ822566
- Sequence Identity:
- Total samples: 1762
- soil counts: 292
- aquatic counts: 148
- animal counts: 916
- plant counts: 406
Safety information
risk assessment
- @ref: 16262
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16262
- description: Brachybacterium phenoliresistens strain phenol-A 16S ribosomal RNA gene, partial sequence
- accession: DQ822566
- length: 1456
- database: ena
- NCBI tax ID: 396014
GC content
| @ref | GC-content | method |
|---|---|---|
| 16262 | 70.8 | high performance liquid chromatography (HPLC) |
| 32133 | 70.8 |
External links
@ref: 16262
culture collection no.: DSM 22299, BCRC 17589, JCM 15157, LMG 23707
straininfo link
- @ref: 73425
- straininfo: 291592
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17978239 | Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. | Chou JH, Lin KY, Lin MC, Sheu SY, Wei YH, Arun AB, Young CC, Chen WM | Int J Syst Evol Microbiol | 10.1099/ijs.0.65019-0 | 2007 | Actinomycetales/chemistry/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Molecular Sequence Data, *Petroleum, Phenols/pharmacology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Pollutants, Species Specificity, Taiwan | Genetics |
| Phylogeny | 26311250 | Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch. | Liu Y, Zhai L, Yao S, Cao Y, Cao Y, Zhang X, Su J, Ge Y, Zhao R, Cheng C | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000559 | 2015 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Morinda/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 16262 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22299) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22299 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 32133 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28377 | 28776041 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 73425 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID291592.1 | StrainInfo: A central database for resolving microbial strain identifiers |