Strain identifier

BacDive ID: 39

Type strain: Yes

Species: Belnapia rosea

Strain history: <- Y.-Q. Zhang, Inst. Med. Biotechnol., Chinese Academy of Med. Sci. and Peking Union Med. College, Beijing; CPCC 100156 <- R. Jin

NCBI tax ID(s): 938405 (species)

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General

@ref: 16783

BacDive-ID: 39

DSM-Number: 23312

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Belnapia rosea DSM 23312 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 938405
  • Matching level: species

strain history

  • @ref: 16783
  • history: <- Y.-Q. Zhang, Inst. Med. Biotechnol., Chinese Academy of Med. Sci. and Peking Union Med. College, Beijing; CPCC 100156 <- R. Jin

doi: 10.13145/bacdive39.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Belnapia
  • species: Belnapia rosea
  • full scientific name: Belnapia rosea Jin et al. 2012

@ref: 16783

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Belnapia

species: Belnapia rosea

full scientific name: Belnapia rosea Jin et al. 2012

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23242negative1.8 µm1.8 µmcoccus-shapedno
69480negative100

colony morphology

  • @ref: 23242
  • colony color: pink to rose, opaque
  • colony shape: circular
  • incubation period: 3 days
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16783R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23242Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
16783positivegrowth28mesophilic
23242positivemaximum37mesophilic
23242positiveminimum20psychrophilic
23242positiveoptimum28-32mesophilic

culture pH

@refabilitytypepH
23242positiveminimum5
23242positivemaximum8
23242positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23242
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.992

halophily

  • @ref: 23242
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 1 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
23242casein-degradation
23242starch-degradation28017
23242glucose-fermentation17234
23242cellulose-hydrolysis62968
23242gelatin-hydrolysis5291
23242D-arabinose+builds acid from17108
23242D-fructose+builds acid from15824
23242D-fucose+builds acid from28847
23242D-lyxose+builds acid from62318
23242D-sorbose+builds acid from17317
23242galactitol+builds acid from16813
23242inulin+builds acid from15443
23242L-arabinose+builds acid from30849
23242L-rhamnose+builds acid from62345
23242L-xylose+builds acid from65328
232422-oxoglutarate+carbon source30916
232423-hydroxybutyrate+carbon source37054
232423-O-methyl-D-glucose+carbon source73918
23242alpha-D-glucose+carbon source17925
23242D-fructose 6-phosphate+carbon source78697
23242D-fucose+carbon source28847
23242D-galactose+carbon source12936
23242D-glucose 6-phosphate+carbon source14314
23242D-glucuronate+carbon source15748
23242glucuronamide+carbon source32323
23242L-fucose+carbon source18287
23242L-rhamnose+carbon source62345
23242malate+carbon source25115
23242alanine+nitrogen source16449
23242arginine+nitrogen source29016
23242glutamate+nitrogen source29987
23242glutamine+nitrogen source28300
23242isoleucine+nitrogen source24898
23242leucine+nitrogen source25017
23242lysine+nitrogen source25094
23242potassium nitrate+nitrogen source63043
23242threonine+nitrogen source26986
23242tryptophan+nitrogen source27897
23242tyrosine+nitrogen source18186
23242valine+nitrogen source27266
23242nitrate+reduction17632

metabolite production

@refChebi-IDmetaboliteproduction
2324216136hydrogen sulfideno
2324215688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2324215688acetoin-
2324217234glucose-

enzymes

@refvalueactivityec
23242acid phosphatase+3.1.3.2
23242alkaline phosphatase+3.1.3.1
23242alpha-chymotrypsin+3.4.21.1
23242alpha-fucosidase-3.2.1.51
23242alpha-galactosidase-3.2.1.22
23242alpha-glucosidase-3.2.1.20
23242alpha-mannosidase-3.2.1.24
23242beta-galactosidase-3.2.1.23
23242beta-glucosidase-3.2.1.21
23242beta-glucuronidase-3.2.1.31
23242catalase+1.11.1.6
23242cystine arylamidase+3.4.11.3
23242cytochrome oxidase-1.9.3.1
23242esterase (C 4)+
23242esterase lipase (C 8)+
23242leucine arylamidase+3.4.11.1
23242lipase (C 14)-
23242N-acetyl-beta-glucosaminidase-3.2.1.52
23242naphthol-AS-BI-phosphohydrolase+
23242trypsin+3.4.21.4
23242valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionisolation procedure
16783forest soilHainan IslandChinaCHNAsia
23242YM agar4.0 g yeast extract (Difco), 10.0 g malt extract (Difco), 4.0 g glucose (Difco), 15.0 g agar and 1 l distilled water, pH 7.2dilution plating

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_65755.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_266;96_3099;97_3800;98_46427;99_65755&stattab=map
  • Last taxonomy: Belnapia rosea subclade
  • 16S sequence: HQ641379
  • Sequence Identity:
  • Total samples: 2472
  • soil counts: 1033
  • aquatic counts: 326
  • animal counts: 952
  • plant counts: 161

Safety information

risk assessment

  • @ref: 16783
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16783
  • description: Belnapia rosea strain CPCC 100156 16S ribosomal RNA gene, partial sequence
  • accession: HQ641379
  • length: 1473
  • database: ena
  • NCBI tax ID: 938405

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Belnapia rosea CPCC 100156GCA_900101615scaffoldncbi938405
66792Belnapia rosea CGMCC 1.10758GCA_900104205scaffoldncbi938405
66792Belnapia rosea strain CGMCC 1.10758938405.4wgspatric938405
66792Belnapia rosea strain CPCC 100156938405.3wgspatric938405
66792Belnapia rosea CGMCC 1.107582596583546draftimg938405

GC content

@refGC-contentmethod
1678370.3thermal denaturation, midpoint method (Tm)
2324270.30thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileyes56.086yes
flagellatedno87.602yes
gram-positiveno98.438no
anaerobicno97.842yes
aerobicyes89.18yes
halophileno94.023yes
spore-formingno92.328no
glucose-fermentno90.248yes
thermophileno91.974yes
glucose-utilyes87.702yes

External links

@ref: 16783

culture collection no.: DSM 23312, CGMCC 1.10758, CPCC 100156

straininfo link

  • @ref: 69724
  • straininfo: 401611

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21551325Description of Belnapia rosea sp. nov. and emended description of the genus Belnapia Reddy et al. 2006.Jin R, Su J, Liu HY, Wei YZ, Li QP, Zhang YQ, Yu LYInt J Syst Evol Microbiol10.1099/ijs.0.031021-02011Acetobacteraceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Benzoquinones/analysis, China, Cluster Analysis, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Temperature, TreesGenetics
Phylogeny23024144Belnapia soli sp. nov., a proteobacterium isolated from grass soil.Jin L, Lee HG, No KJ, Ko SR, Kim HS, Ahn CY, Oh HMInt J Syst Evol Microbiol10.1099/ijs.0.045302-02012Acetobacteraceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analysisGenetics
Phylogeny33548024Siccirubricoccus phaeus sp. nov., isolated from oil reservoir water and emended description of the genus Siccirubricoccus.Li FL, Zhang YX, Zhang YK, Chen WF, Li WJ, Wang LAntonie Van Leeuwenhoek10.1007/s10482-021-01516-82021Acetobacteraceae, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids, Nucleic Acid Hybridization, *Oil and Gas Fields, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *WaterTranscriptome
Phylogeny34292142Belnapia mucosa sp. nov. and Belnapia arida sp. nov., isolated from desert biocrust.Molina-Menor E, Vidal-Verdu A, Satari L, Calonge-Garcia A, Pascual J, Pereto J, Porcar MInt J Syst Evol Microbiol10.1099/ijsem.0.0048372021Acetobacteraceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, SpainTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16783Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23312)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23312
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23242Rong Jin, Jing Su, Hong-Yu Liu, Yu-Zhen Wei, Qiu-Ping Li, Yu-Qin Zhang, Li-Yan Yu10.1099/ijs.0.031021-0Description of Belnapia rosea sp. nov. and emended description of the genus Belnapia Reddy et al. 2006IJSEM 62: 705-709 201221551325
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69724Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401611.1StrainInfo: A central database for resolving microbial strain identifiers