Strain identifier
BacDive ID: 3788
Type strain:
Species: Eisenibacter elegans
Strain Designation: Fx a2, NZ-1
Strain history: CIP <- 1996, IFO <- 1990, ATCC <- R.A. Lewin: strain NZ-1
NCBI tax ID(s): 1121902 (strain), 997 (species)
General
@ref: 1354
BacDive-ID: 3788
DSM-Number: 3317
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative
description: Eisenibacter elegans Fx a2 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from hot spring.
NCBI tax id
NCBI tax id | Matching level |
---|---|
997 | species |
1121902 | strain |
strain history
@ref | history |
---|---|
1354 | <- H. Reichenbach, Fx a2 <- ATCC <- R.A. Lewin, NZ-1 |
67770 | IAM 14319 <-- IFO 15055 <-- ATCC 23112 <-- R. A. Lewin NZ-1. |
119002 | CIP <- 1996, IFO <- 1990, ATCC <- R.A. Lewin: strain NZ-1 |
doi: 10.13145/bacdive3788.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Cytophagia
- order: Cytophagales
- family: Microscillaceae
- genus: Eisenibacter
- species: Eisenibacter elegans
- full scientific name: Eisenibacter elegans (Reichenbach 1989 ex Lewin 1969) Hahnke et al. 2017
synonyms
- @ref: 20215
- synonym: Flexibacter elegans
@ref: 1354
domain: Bacteria
phylum: Bacteroidetes
class: Cytophagia
order: Cytophagales
family: Microscillaceae
genus: Eisenibacter
species: Eisenibacter elegans
full scientific name: Eisenibacter elegans (Reichenbach 1989) Hahnke et al. 2017
strain designation: Fx a2, NZ-1
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.999 | ||
119002 | negative | rod-shaped | no |
colony morphology
- @ref: 119002
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
66793 | EM_DSM_3317_1.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_3317_2.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_3317_3.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_3317_4.jpg | electron microscopic image | © HZI/Manfred Rohde |
66793 | EM_DSM_3317_5.jpg | electron microscopic image | © HZI/Manfred Rohde |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
1354 | FLEXIBACTER MEDIUM (DSMZ Medium 344) | yes | https://mediadive.dsmz.de/medium/344 | Name: FLEXIBACTER MEDIUM (DSMZ Medium 344) Composition: Agar 15.0 g/l Na glutamate 5.0 g/l Glucose 2.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 1.0 g/l Distilled water |
42265 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
119002 | CIP Medium 33 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=33 | |
119002 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1354 | positive | growth | 30 | mesophilic |
42265 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
119002 | positive | growth | 25-45 | |
119002 | no | growth | 5 | psychrophilic |
119002 | no | growth | 15 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 119002
- oxygen tolerance: facultative anaerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.904 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
119002 | NaCl | positive | growth | 0-2 % |
119002 | NaCl | no | growth | 4 % |
119002 | NaCl | no | growth | 6 % |
119002 | NaCl | no | growth | 8 % |
119002 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
119002 | 4853 | esculin | - | hydrolysis |
119002 | 606565 | hippurate | + | hydrolysis |
119002 | 17632 | nitrate | - | reduction |
119002 | 16301 | nitrite | - | reduction |
119002 | 15792 | malonate | - | assimilation |
119002 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 119002
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 119002
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
119002 | 15688 | acetoin | - | |
119002 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
119002 | oxidase | + | |
119002 | beta-galactosidase | + | 3.2.1.23 |
119002 | alcohol dehydrogenase | - | 1.1.1.1 |
119002 | gelatinase | + | |
119002 | amylase | - | |
119002 | DNase | - | |
119002 | caseinase | - | 3.4.21.50 |
119002 | catalase | - | 1.11.1.6 |
119002 | tween esterase | + | |
119002 | gamma-glutamyltransferase | + | 2.3.2.2 |
119002 | lecithinase | - | |
119002 | lipase | - | |
119002 | lysine decarboxylase | + | 4.1.1.18 |
119002 | ornithine decarboxylase | - | 4.1.1.17 |
119002 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
119002 | protease | - | |
119002 | tryptophan deaminase | - | |
119002 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119002 | - | + | + | + | + | + | + | + | + | + | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119002 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
1354 | hot spring | Rotorua | New Zealand | NZL | Australia and Oceania |
67770 | Hot spring | Rotorua | New Zealand | NZL | Australia and Oceania |
119002 | Environment, Hot spring | Rotorua | New Zealand | NZL | Australia and Oceania |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_19313.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2259;96_9849;97_11877;98_14599;99_19313&stattab=map
- Last taxonomy: Cytophagales
- 16S sequence: NR_113723
- Sequence Identity:
- Total samples: 9
- soil counts: 3
- aquatic counts: 3
- animal counts: 3
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
1354 | 1 | Risk group (German classification) |
119002 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Flexibacter elegans 16S ribosomal RNA | M58783 | 1459 | ena | 997 |
20218 | Flexibacter elegans gene for 16S rRNA, strain:IFO 15055 | AB078048 | 1461 | ena | 997 |
20218 | Flexibacter elegans gene for 16S rRNA, partial sequence, strain: NBRC 15055 | AB680760 | 1431 | ena | 997 |
1354 | Eisenibacter elegans strain NBRC 15055 16S ribosomal RNA, partial sequence | NR_113723 | 1431 | nuccore | 997 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Eisenibacter elegans DSM 3317 | 1121902.6 | wgs | patric | 1121902 |
66792 | Eisenibacter elegans DSM 3317 | 2524614599 | draft | img | 1121902 |
67770 | Eisenibacter elegans DSM 3317 | GCA_000430505 | scaffold | ncbi | 1121902 |
GC content
- @ref: 1354
- GC-content: 47.4
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 88.217 | no |
flagellated | no | 96.407 | no |
gram-positive | no | 98.727 | no |
anaerobic | no | 97.509 | no |
halophile | no | 75.45 | no |
spore-forming | no | 91.492 | no |
thermophile | no | 93.856 | yes |
glucose-util | yes | 66.512 | no |
aerobic | yes | 86.034 | no |
glucose-ferment | no | 91.819 | no |
External links
@ref: 1354
culture collection no.: DSM 3317, ATCC 23112, IAM 14319, IFO 15055, NBRC 15055, JCM 21159, ATCC 19594, ATCC 23180, CIP 104801, LMG 10750, LMG 3988, NCIMB 1385
straininfo link
- @ref: 73300
- straininfo: 9860
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1354 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3317) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3317 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
42265 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16749 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
73300 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID9860.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119002 | Curators of the CIP | Collection of Institut Pasteur (CIP 104801) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104801 |