Strain identifier

BacDive ID: 3788

Type strain: Yes

Species: Eisenibacter elegans

Strain Designation: Fx a2, NZ-1

Strain history: CIP <- 1996, IFO <- 1990, ATCC <- R.A. Lewin: strain NZ-1

NCBI tax ID(s): 1121902 (strain), 997 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1354

BacDive-ID: 3788

DSM-Number: 3317

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative

description: Eisenibacter elegans Fx a2 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from hot spring.

NCBI tax id

NCBI tax idMatching level
997species
1121902strain

strain history

@refhistory
1354<- H. Reichenbach, Fx a2 <- ATCC <- R.A. Lewin, NZ-1
67770IAM 14319 <-- IFO 15055 <-- ATCC 23112 <-- R. A. Lewin NZ-1.
119002CIP <- 1996, IFO <- 1990, ATCC <- R.A. Lewin: strain NZ-1

doi: 10.13145/bacdive3788.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Microscillaceae
  • genus: Eisenibacter
  • species: Eisenibacter elegans
  • full scientific name: Eisenibacter elegans (Reichenbach 1989 ex Lewin 1969) Hahnke et al. 2017
  • synonyms

    • @ref: 20215
    • synonym: Flexibacter elegans

@ref: 1354

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Microscillaceae

genus: Eisenibacter

species: Eisenibacter elegans

full scientific name: Eisenibacter elegans (Reichenbach 1989) Hahnke et al. 2017

strain designation: Fx a2, NZ-1

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
119002negativerod-shapedno

colony morphology

  • @ref: 119002

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_3317_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_3317_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_3317_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_3317_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_3317_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1354FLEXIBACTER MEDIUM (DSMZ Medium 344)yeshttps://mediadive.dsmz.de/medium/344Name: FLEXIBACTER MEDIUM (DSMZ Medium 344) Composition: Agar 15.0 g/l Na glutamate 5.0 g/l Glucose 2.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 1.0 g/l Distilled water
42265MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119002CIP Medium 33yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=33
119002CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1354positivegrowth30mesophilic
42265positivegrowth25mesophilic
67770positivegrowth30mesophilic
119002positivegrowth25-45
119002nogrowth5psychrophilic
119002nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119002
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.904

halophily

@refsaltgrowthtested relationconcentration
119002NaClpositivegrowth0-2 %
119002NaClnogrowth4 %
119002NaClnogrowth6 %
119002NaClnogrowth8 %
119002NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1190024853esculin-hydrolysis
119002606565hippurate+hydrolysis
11900217632nitrate-reduction
11900216301nitrite-reduction
11900215792malonate-assimilation
11900217632nitrate+respiration

antibiotic resistance

  • @ref: 119002
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119002
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11900215688acetoin-
11900217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119002oxidase+
119002beta-galactosidase+3.2.1.23
119002alcohol dehydrogenase-1.1.1.1
119002gelatinase+
119002amylase-
119002DNase-
119002caseinase-3.4.21.50
119002catalase-1.11.1.6
119002tween esterase+
119002gamma-glutamyltransferase+2.3.2.2
119002lecithinase-
119002lipase-
119002lysine decarboxylase+4.1.1.18
119002ornithine decarboxylase-4.1.1.17
119002phenylalanine ammonia-lyase-4.3.1.24
119002protease-
119002tryptophan deaminase-
119002urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119002-+++++++++++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119002---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1354hot springRotoruaNew ZealandNZLAustralia and Oceania
67770Hot springRotoruaNew ZealandNZLAustralia and Oceania
119002Environment, Hot springRotoruaNew ZealandNZLAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_19313.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2259;96_9849;97_11877;98_14599;99_19313&stattab=map
  • Last taxonomy: Cytophagales
  • 16S sequence: NR_113723
  • Sequence Identity:
  • Total samples: 9
  • soil counts: 3
  • aquatic counts: 3
  • animal counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
13541Risk group (German classification)
1190021Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Flexibacter elegans 16S ribosomal RNAM587831459ena997
20218Flexibacter elegans gene for 16S rRNA, strain:IFO 15055AB0780481461ena997
20218Flexibacter elegans gene for 16S rRNA, partial sequence, strain: NBRC 15055AB6807601431ena997
1354Eisenibacter elegans strain NBRC 15055 16S ribosomal RNA, partial sequenceNR_1137231431nuccore997

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Eisenibacter elegans DSM 33171121902.6wgspatric1121902
66792Eisenibacter elegans DSM 33172524614599draftimg1121902
67770Eisenibacter elegans DSM 3317GCA_000430505scaffoldncbi1121902

GC content

  • @ref: 1354
  • GC-content: 47.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno88.217no
flagellatedno96.407no
gram-positiveno98.727no
anaerobicno97.509no
halophileno75.45no
spore-formingno91.492no
thermophileno93.856yes
glucose-utilyes66.512no
aerobicyes86.034no
glucose-fermentno91.819no

External links

@ref: 1354

culture collection no.: DSM 3317, ATCC 23112, IAM 14319, IFO 15055, NBRC 15055, JCM 21159, ATCC 19594, ATCC 23180, CIP 104801, LMG 10750, LMG 3988, NCIMB 1385

straininfo link

  • @ref: 73300
  • straininfo: 9860

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1354Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3317)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3317
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42265Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16749
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
73300Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9860.1StrainInfo: A central database for resolving microbial strain identifiers
119002Curators of the CIPCollection of Institut Pasteur (CIP 104801)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104801