Strain identifier
BacDive ID: 3784
Type strain: 
Species: Flectobacillus major
Strain history: <- VKM <- B.V. Gromov (Microcyclus major)
NCBI tax ID(s): 929703 (strain), 103 (species)
version 8.1 (current version)
General
@ref: 2079
BacDive-ID: 3784
DSM-Number: 103
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative
description: Flectobacillus major DSM 103 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Algal culture.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 103 | species |
| 929703 | strain |
strain history
- @ref: 2079
- history: <- VKM <- B.V. Gromov (Microcyclus major)
doi: 10.13145/bacdive3784.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Cytophagia
- order: Cytophagales
- family: Spirosomataceae
- genus: Flectobacillus
- species: Flectobacillus major
- full scientific name: Flectobacillus major (Gromov 1963) Larkin et al. 1977 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Microcyclus major
@ref: 2079
domain: Bacteria
phylum: Bacteroidetes
class: Cytophagia
order: Cytophagales
family: Spirosomaceae
genus: Flectobacillus
species: Flectobacillus major
full scientific name: Flectobacillus major (Gromov 1963) Larkin et al. 1977 emend. Hahnke et al. 2016
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.999
multimedia
- @ref: 66793
- multimedia content: EM_DSM_103_1.jpg
- caption: electron microscopic image
- intellectual property rights: © HZI/Manfred Rohde
Culture and growth conditions
culture medium
- @ref: 2079
- name: ANCYLOBACTER - SPIROSOMA MEDIUM (DSMZ Medium 7)
- growth: yes
- link: https://mediadive.dsmz.de/medium/7
- composition: Name: ANCYLOBACTER - SPIROSOMA MEDIUM (DSMZ Medium 7) Composition: Agar 15.0 g/l Yeast extract 1.0 g/l Peptone 1.0 g/l Glucose 1.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 2079 | positive | growth | 26 | mesophilic |
| 46164 | positive | growth | 20-30 |
Physiology and metabolism
oxygen tolerance
- @ref: 46164
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.98 |
Isolation, sampling and environmental information
isolation
- @ref: 46164
- sample type: Algal culture
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Laboratory | #Lab enrichment |
| #Host | #Algae |
Safety information
risk assessment
- @ref: 2079
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 2079
- description: Flectobacillus major strain ATCC 29496 16S ribosomal RNA gene, partial sequence
- accession: M62787
- length: 1476
- database: ena
- NCBI tax ID: 103
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Flectobacillus major DSM 103 | GCA_000427405 | scaffold | ncbi | 929703 |
| 66792 | Flectobacillus major DSM 103 | 929703.3 | wgs | patric | 929703 |
| 66792 | Flectobacillus major VKMB-859, DSM 103 | 2509601041 | draft | img | 929703 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 2079 | 39.5 | |
| 2079 | 37.8 | sequence analysis |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 92.328 | no |
| flagellated | no | 97.165 | no |
| gram-positive | no | 98.447 | no |
| anaerobic | no | 99.192 | yes |
| aerobic | yes | 86.361 | no |
| halophile | no | 96.092 | no |
| spore-forming | no | 95.638 | no |
| glucose-util | yes | 83.201 | no |
| thermophile | no | 98.557 | yes |
| glucose-ferment | no | 90.237 | no |
External links
@ref: 2079
culture collection no.: DSM 103, ATCC 29496, BKM B-859, VKM B-859, CCUG 14833, CCM 2921
straininfo link
- @ref: 73296
- straininfo: 7686
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19622645 | Flectobacillus roseus sp. nov., isolated from freshwater in Taiwan. | Sheu SY, Chiu TF, Cho NT, Chou JH, Sheu DS, Arun AB, Young CC, Chen CA, Wang JT, Chen WM | Int J Syst Evol Microbiol | 10.1099/ijs.0.009738-0 | 2009 | Cytophagaceae/chemistry/classification/genetics/*isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Taiwan | Genetics |
| Phylogeny | 20008110 | Siphonobacter aquaeclarae gen. nov., sp. nov., a novel member of the family 'Flexibacteraceae', phylum Bacteroidetes. | Tancsics A, Keki Z, Marialigeti K, Schumann P, Toth EM | Int J Syst Evol Microbiol | 10.1099/ijs.0.019398-0 | 2009 | Base Composition, Cytophagaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/metabolism, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, Power Plants, RNA, Ribosomal, 16S/genetics | Metabolism |
| Phylogeny | 26297007 | Flectobacillus rhizosphaerae sp. nov., isolated from the rhizosphere soil of Oryza sativa (L.), and emended description of the genus Flectobacillus. | Ramaprasad EVV, Sasikala C, Ramana CV | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000432 | 2015 | Bacterial Typing Techniques, Base Composition, Cytophagaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2079 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 46164 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 14833) | https://www.ccug.se/strain?id=14833 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66793 | Mukherjee et al. | 10.1038/nbt.3886 | GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life | 35: 676-683 2017 | 28604660 | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 73296 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID7686.1 | StrainInfo: A central database for resolving microbial strain identifiers |