Archive BacDiveID:10.13145/bacdive3755.20230509.8.1

Strain identifier

BacDive ID: 3755

Type strain:

Species: Anaeromyxobacter dehalogenans

Strain Designation: 2CP-1

Strain history: <- S. H. Thomas, Georgia Institute of Technology, School of Civil and Environmental Engineering, Atlanta <- R. A. Sanford, Department of Civil Environmental Engineering, University of Illinois <- J. R. Cole, Center for Microbial Ecol. , Michigan State Univ., USA

NCBI tax ID(s): 161493 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 16008

BacDive-ID: 3755

DSM-Number: 21875

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Anaeromyxobacter dehalogenans 2CP-1 is an anaerobe, mesophilic bacterium that was isolated from stream sediment near Lansing, Michigan.

NCBI tax id

NCBI tax id: 161493
Matching level: species

strain history

@ref: 16008
history: <- S. H. Thomas, Georgia Institute of Technology, School of Civil and Environmental Engineering, Atlanta <- R. A. Sanford, Department of Civil Environmental Engineering, University of Illinois <- J. R. Cole, Center for Microbial Ecol. , Michigan State Univ., USA

doi: 10.13145/bacdive3755.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Deltaproteobacteria
order: Myxococcales
family: Anaeromyxobacteraceae
genus: Anaeromyxobacter
species: Anaeromyxobacter dehalogenans
full scientific name: Anaeromyxobacter dehalogenans Sanford et al. 2002

@ref: 16008

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Anaeromyxobacteraceae

genus: Anaeromyxobacter

species: Anaeromyxobacter dehalogenans

full scientific name: Anaeromyxobacter dehalogenans Sanford et al. 2002

strain designation: 2CP-1

type strain: yes

Morphology

colony morphology

@ref: 16008
incubation period: >14 days

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
16008	R2A MEDIUM (DSMZ Medium 830)	yes	https://mediadive.dsmz.de/medium/830	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
16008	ANAEROMYXOBACTER-MEDIUM (DSMZ Medium 1200)	yes	https://mediadive.dsmz.de/medium/1200	Name: ANAEROMYXOBACTER-MEDIUM (DSMZ Medium 1200) Composition: NaHCO3 4.54545 g/l NaCl 1.00563 g/l MgCl2 x 6 H2O 0.502815 g/l Na-acetate 0.402253 g/l Disodium fumarate 0.363636 g/l NH4Cl 0.301689 g/l KCl 0.301689 g/l KH2PO4 0.201126 g/l DL-Dithiothreitol 0.14 g/l CaCl2 x 2 H2O 0.0150845 g/l Na2S x H2O 0.0145455 g/l L-Cysteine 0.0136364 g/l HCl 0.00193584 g/l FeSO4 x 7 H2O 0.00149648 g/l NaOH 0.00100563 g/l H3BO3 0.000299295 g/l CoCl2 x 6 H2O 0.000189553 g/l Pyridoxine hydrochloride 0.000181818 g/l MnCl2 x 4 H2O 9.97651e-05 g/l Nicotinic acid 9.09091e-05 g/l D-Calcium pantothenate 9.09091e-05 g/l p-Aminobenzoic acid 9.09091e-05 g/l Lipoic acid 9.09091e-05 g/l Thiamine-HCl x 2 H2O 9.09091e-05 g/l Riboflavin 9.09091e-05 g/l ZnCl2 6.98355e-05 g/l Biotin 3.63636e-05 g/l Folic acid 3.63636e-05 g/l Na2MoO4 x 2 H2O 3.59154e-05 g/l NiCl2 x 6 H2O 2.39436e-05 g/l Na2WO4 x 2 H2O 8.04505e-06 g/l Na2SeO3 x 5 H2O 6.03379e-06 g/l CuCl2 x 2 H2O 1.9953e-06 g/l Vitamin B12 1.81818e-06 g/l Resazurin Distilled water

culture temp

@ref: 16008
growth: positive
type: growth
temperature: 28
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
16008	anaerobe
16008	microaerophile

Isolation, sampling and environmental information

isolation

@ref: 16008
sample type: stream sediment near Lansing, Michigan
geographic location: Lansing, Michigan
country: USA
origin.country: USA
continent: North America

isolation source categories

Cat1: #Environmental
Cat2: #Aquatic
Cat3: #Sediment

taxonmaps

@ref: 69479
File name: preview.99_3121.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_371;96_1627;97_1947;98_2395;99_3121&stattab=map
Last taxonomy: Anaeromyxobacter
16S sequence: AF382396
Sequence Identity:
Total samples: 10224
soil counts: 6429
aquatic counts: 2438
animal counts: 420
plant counts: 937

Safety information

risk assessment

@ref: 16008
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 16008
description: Anaeromyxobacter dehalogenans strain 2CP-1 16S ribosomal RNA gene, partial sequence
accession: AF382396
length: 1549
database: ena
NCBI tax ID: 455488

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Anaeromyxobacter dehalogenans 2CP-1 2CP1	GCA_000022145	complete	ncbi	455488
66792	Anaeromyxobacter dehalogenans 2CP-1	455488.5	complete	patric	455488
66792	Anaeromyxobacter dehalogenans 2CP-1	643348507	complete	img	455488

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	yes	81.192	no
flagellated	no	79.294	no
gram-positive	no	98.37	no
anaerobic	no	72.15	no
aerobic	no	83.018	yes
halophile	no	92.488	no
spore-forming	no	89.384	no
glucose-util	yes	51.289	no
thermophile	no	87.262	yes
glucose-ferment	no	92.444	no

External links

@ref: 16008

culture collection no.: DSM 21875, ATCC BAA 258

straininfo link

@ref: 73267
straininfo: 87742

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	11823233	Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an aryl-halorespiring facultative anaerobic myxobacterium.	Sanford RA, Cole JR, Tiedje JM	Appl Environ Microbiol	10.1128/AEM.68.2.893-900.2002	2002	Acetates/metabolism, Anaerobiosis, Chlorine/metabolism, Chlorophenols/metabolism, DNA, Ribosomal/analysis, Deltaproteobacteria/classification/genetics/growth & development/isolation & purification/physiology, Geologic Sediments/microbiology, Hydrogen/metabolism, Molecular Sequence Data, Nitrates/metabolism, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology	Metabolism
	19719643	Strain FAc12, a dissimilatory iron-reducing member of the Anaeromyxobacter subgroup of Myxococcales.	Treude N, Rosencrantz D, Liesack W, Schnell S	FEMS Microbiol Ecol	10.1016/S0168-6496(03)00048-5	2003
Phylogeny	23709511	Release of arsenic from soil by a novel dissimilatory arsenate-reducing bacterium, Anaeromyxobacter sp. strain PSR-1.	Kudo K, Yamaguchi N, Makino T, Ohtsuka T, Kimura K, Dong DT, Amachi S	Appl Environ Microbiol	10.1128/AEM.00693-13	2013	Arsenates/metabolism, Arsenic/metabolism, Denaturing Gradient Gel Electrophoresis, Molecular Sequence Data, Myxococcales/genetics/physiology, Oxidation-Reduction, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics/metabolism, Sequence Analysis, DNA, Sequence Homology, Soil Microbiology, Soil Pollutants/metabolism, Species Specificity	Metabolism
	25505896	Genomic analyses of bacterial porin-cytochrome gene clusters.	Shi L, Fredrickson JK, Zachara JM	Front Microbiol	10.3389/fmicb.2014.00657	2014
Phylogeny	36191051	Anaeromyxobacter oryzae sp. nov., Anaeromyxobacter diazotrophicus sp. nov. and Anaeromyxobacter paludicola sp. nov., isolated from paddy soils.	Itoh H, Xu Z, Mise K, Masuda Y, Ushijima N, Hayakawa C, Shiratori Y, Senoo K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005546	2022	Agar, Amino Acids, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nitrogenase/genetics, Nucleic Acid Hybridization, Nucleotides, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil	Transcriptome

Reference

@id	authors	catalogue	doi/url	title
16008	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21875)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-21875
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
73267	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID87742.1	StrainInfo: A central database for resolving microbial strain identifiers