Strain identifier
BacDive ID: 363
Type strain:
Species: Pusillimonas soli
Strain Designation: MJ07
Strain history: <- MJ Lee, H-Plus Eco, Korea
NCBI tax ID(s): 659016 (species)
General
@ref: 17962
BacDive-ID: 363
DSM-Number: 25264
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Pusillimonas soli MJ07 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from farm soil.
NCBI tax id
- NCBI tax id: 659016
- Matching level: species
strain history
@ref | history |
---|---|
17962 | <- KCTC <- M. Lee, H-Plus Eco Ltd., KRIBB, Daejeon |
67770 | M. J. Lee MJ07. |
67771 | <- MJ Lee, H-Plus Eco, Korea |
doi: 10.13145/bacdive363.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Alcaligenaceae
- genus: Pusillimonas
- species: Pusillimonas soli
- full scientific name: Pusillimonas soli Lee et al. 2010
synonyms
- @ref: 20215
- synonym: Allopusillimonas soli
@ref: 17962
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Alcaligenaceae
genus: Pusillimonas
species: Pusillimonas soli
full scientific name: Pusillimonas soli Lee et al. 2010
strain designation: MJ07
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
23277 | negative | 0.7-1.0 µm | 0.3-0.5 µm | rod-shaped | yes | |
67771 | negative | |||||
69480 | yes | 94.589 | ||||
69480 | negative | 99.994 |
multimedia
- @ref: 17962
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_25264.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 17962
- name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
- growth: yes
- link: https://mediadive.dsmz.de/medium/381
- composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
17962 | positive | growth | 28 | mesophilic |
23277 | positive | optimum | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
67771 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
23277 | positive | growth | 5.0-9.0 | alkaliphile |
23277 | positive | optimum | 6.5-7.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 67771
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
23277 | no | |
69481 | no | 100 |
69480 | no | 99.994 |
observation
- @ref: 67770
- observation: quinones: Q-8
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23277 | 27689 | decanoate | - | assimilation |
23277 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
23277 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
23277 | 27613 | amygdalin | - | builds acid from |
23277 | 15963 | ribitol | - | builds acid from |
23277 | 17108 | D-arabinose | - | builds acid from |
23277 | 18333 | D-arabitol | - | builds acid from |
23277 | 17057 | cellobiose | - | builds acid from |
23277 | 15824 | D-fructose | - | builds acid from |
23277 | 28847 | D-fucose | - | builds acid from |
23277 | 12936 | D-galactose | - | builds acid from |
23277 | 17716 | lactose | - | builds acid from |
23277 | 62318 | D-lyxose | - | builds acid from |
23277 | 16899 | D-mannitol | - | builds acid from |
23277 | 16024 | D-mannose | - | builds acid from |
23277 | 6731 | melezitose | - | builds acid from |
23277 | 28053 | melibiose | - | builds acid from |
23277 | 16634 | raffinose | - | builds acid from |
23277 | 17992 | sucrose | - | builds acid from |
23277 | 17924 | D-sorbitol | - | builds acid from |
23277 | 16443 | D-tagatose | - | builds acid from |
23277 | 32528 | turanose | - | builds acid from |
23277 | 65327 | D-xylose | - | builds acid from |
23277 | 16813 | galactitol | - | builds acid from |
23277 | 17113 | erythritol | - | builds acid from |
23277 | 4853 | esculin | - | builds acid from |
23277 | 28066 | gentiobiose | - | builds acid from |
23277 | 28087 | glycogen | - | builds acid from |
23277 | 17268 | myo-inositol | - | builds acid from |
23277 | 15443 | inulin | - | builds acid from |
23277 | 18403 | L-arabitol | - | builds acid from |
23277 | 18287 | L-fucose | - | builds acid from |
23277 | 62345 | L-rhamnose | - | builds acid from |
23277 | 17266 | L-sorbose | - | builds acid from |
23277 | 65328 | L-xylose | - | builds acid from |
23277 | 17306 | maltose | - | builds acid from |
23277 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
23277 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
23277 | 506227 | N-acetylglucosamine | - | builds acid from |
23277 | 17814 | salicin | - | builds acid from |
23277 | 28017 | starch | - | builds acid from |
23277 | 27082 | trehalose | - | builds acid from |
23277 | 17151 | xylitol | - | builds acid from |
23277 | 16808 | 2-dehydro-D-gluconate | - | growth |
23277 | 17426 | 5-dehydro-D-gluconate | - | growth |
23277 | 17634 | D-glucose | - | growth |
23277 | 16899 | D-mannitol | - | growth |
23277 | 16024 | D-mannose | - | growth |
23277 | 24265 | gluconate | - | growth |
23277 | 28087 | glycogen | - | growth |
23277 | 30849 | L-arabinose | - | growth |
23277 | 18287 | L-fucose | - | growth |
23277 | 17306 | maltose | - | growth |
23277 | 28053 | melibiose | - | growth |
23277 | 506227 | N-acetylglucosamine | - | growth |
23277 | 17814 | salicin | - | growth |
23277 | 17992 | sucrose | - | growth |
23277 | casein | - | hydrolysis | |
23277 | 17029 | chitin | - | hydrolysis |
23277 | 16991 | dna | - | hydrolysis |
23277 | 85249 | hydroxyethylcellulose | - | hydrolysis |
23277 | 28017 | starch | - | hydrolysis |
23277 | 37166 | xylan | - | hydrolysis |
23277 | 16193 | 3-hydroxybenzoate | + | assimilation |
23277 | 17128 | adipate | + | assimilation |
23277 | 16947 | citrate | + | assimilation |
23277 | 16988 | D-ribose | + | assimilation |
23277 | 17240 | itaconate | + | assimilation |
23277 | 16977 | L-alanine | + | assimilation |
23277 | 15971 | L-histidine | + | assimilation |
23277 | 17203 | L-proline | + | assimilation |
23277 | 17115 | L-serine | + | assimilation |
23277 | 25115 | malate | + | assimilation |
23277 | 15792 | malonate | + | assimilation |
23277 | 17268 | myo-inositol | + | assimilation |
23277 | 18401 | phenylacetate | + | assimilation |
23277 | 17272 | propionate | + | assimilation |
23277 | 9300 | suberic acid | + | assimilation |
23277 | 31011 | valerate | + | assimilation |
23277 | 18305 | arbutin | + | builds acid from |
23277 | 17634 | D-glucose | + | builds acid from |
23277 | 16988 | D-ribose | + | builds acid from |
23277 | 17924 | D-sorbitol | + | builds acid from |
23277 | 8391 | D-gluconate | + | builds acid from |
23277 | 17754 | glycerol | + | builds acid from |
23277 | 30849 | L-arabinose | + | builds acid from |
23277 | 62345 | L-rhamnose | + | builds acid from |
23277 | 74863 | methyl beta-D-xylopyranoside | + | builds acid from |
23277 | 37054 | 3-hydroxybutyrate | + | growth |
23277 | 30089 | acetate | + | growth |
23277 | 24996 | lactate | + | growth |
23277 | 17632 | nitrate | + | reduction |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23277 | Acid phosphatase | + | 3.1.3.2 |
23277 | Alkaline phosphatase | + | 3.1.3.1 |
23277 | alpha-chymotrypsin | - | 3.4.21.1 |
23277 | alpha-fucosidase | - | 3.2.1.51 |
23277 | alpha-galactosidase | - | 3.2.1.22 |
23277 | alpha-glucosidase | - | 3.2.1.20 |
23277 | alpha-mannosidase | - | 3.2.1.24 |
23277 | beta-galactosidase | - | 3.2.1.23 |
23277 | beta-glucosidase | - | 3.2.1.21 |
23277 | beta-glucuronidase | - | 3.2.1.31 |
23277 | catalase | + | 1.11.1.6 |
23277 | cystine arylamidase | - | 3.4.11.3 |
23277 | cytochrome oxidase | + | 1.9.3.1 |
23277 | esterase (C 4) | + | |
23277 | esterase lipase (C 8) | + | |
23277 | leucine arylamidase | + | 3.4.11.1 |
23277 | lipase (C 14) | - | |
23277 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
23277 | naphthol-AS-BI-phosphohydrolase | + | |
23277 | trypsin | - | 3.4.21.4 |
23277 | valine arylamidase | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
17962 | farm soil | near Daejeon | Republic of Korea | KOR | Asia |
67770 | Farm soil near Daejeon | Republic of Korea | KOR | Asia | |
67771 | From soil | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Engineered | #Agriculture | #Field |
#Engineered | #Food production | #Aquaculture |
Safety information
risk assessment
- @ref: 17962
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17962
- description: Pusillimonas soli strain MJ07 16S ribosomal RNA gene, partial sequence
- accession: GQ241322
- length: 1454
- database: ena
- NCBI tax ID: 659016
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Allopusillimonas soli DSM 25264 | GCA_013416335 | contig | ncbi | 659016 |
66792 | Pusillimonas soli strain DSM 25264 | 659016.5 | wgs | patric | 659016 |
66792 | Pusillimonas soli strain KCTC 22455 | 659016.4 | wgs | patric | 659016 |
66792 | Pusillimonas soli KCTC 22455 | 2891629496 | draft | img | 659016 |
67770 | Allopusillimonas soli KCTC 22455 | GCA_004153445 | scaffold | ncbi | 659016 |
GC content
@ref | GC-content | method |
---|---|---|
17962 | 59.4 | |
23277 | 59.4 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 98.039 | yes |
anaerobic | no | 99.246 | yes |
halophile | no | 74.962 | no |
spore-forming | no | 94.162 | yes |
glucose-util | no | 76.983 | yes |
thermophile | no | 96.73 | yes |
flagellated | no | 73.271 | no |
motile | yes | 86.672 | yes |
aerobic | yes | 90.419 | no |
glucose-ferment | no | 91.875 | no |
External links
@ref: 17962
culture collection no.: DSM 25264, JCM 16386, KCTC 22455
straininfo link
- @ref: 70043
- straininfo: 404651
literature
- topic: Phylogeny
- Pubmed-ID: 19933592
- title: Pusillimonas soli sp. nov., isolated from farm soil.
- authors: Lee M, Woo SG, Chae M, Ten LN
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.020404-0
- year: 2009
- mesh: Alcaligenaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Polyamines/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
17962 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25264) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25264 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23277 | Myungjin Lee, Sung-Geun Woo, Myoungsoo Chae, Leonid N. Ten | 10.1099/ijs.0.020404-0 | Pusillimonas soli sp. nov., isolated from farm soil | IJSEM 60: 2326-2330 2010 | 19933592 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
70043 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID404651.1 | StrainInfo: A central database for resolving microbial strain identifiers |