Strain identifier

BacDive ID: 362

Type strain: Yes

Species: Pusillimonas noertemannii

Strain Designation: BN9

Strain history: CIP <- 2005, P. Kämpfer, Giessen Univ., Giessen, Germany <- R. Rub <- B. Nörtemann: strain BN9

NCBI tax ID(s): 305977 (species)

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General

@ref: 3791

BacDive-ID: 362

DSM-Number: 10065

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Pusillimonas noertemannii BN9 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water of river Elbe.

NCBI tax id

  • NCBI tax id: 305977
  • Matching level: species

strain history

@refhistory
3791<- R. Ruß <- B. Nörtemann; BN9
121324CIP <- 2005, P. Kämpfer, Giessen Univ., Giessen, Germany <- R. Rub <- B. Nörtemann: strain BN9

doi: 10.13145/bacdive362.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Pusillimonas
  • species: Pusillimonas noertemannii
  • full scientific name: Pusillimonas noertemannii Stolz et al. 2005

@ref: 3791

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Pusillimonas

species: Pusillimonas noertemannii

full scientific name: Pusillimonas noertemannii Stolz et al. 2005

strain designation: BN9

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23276negative1-1.5 µm0.5-0.8 µmrod-shapedyesmonotrichous, polar
69480yes93.334
69480negative99.999
121324negativerod-shapedyes

colony morphology

@refcolony colorcolony shapemedium used
23276brownishcircularnutrient agar
121324

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3791NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
3791PLATE COUNT AGAR (DSMZ Medium 464)yeshttps://mediadive.dsmz.de/medium/464Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
3791MINERAL MEDIUM PH 7.25 (DSMZ Medium 465)yeshttps://mediadive.dsmz.de/medium/465Name: MINERAL MEDIUM PH 7.25 (DSMZ Medium 465; with strain-specific modifications) Composition: Na2HPO4 x 2 H2O 3.5 g/l KH2PO4 1.0 g/l None 0.5 g/l (NH4)2SO4 0.5 g/l MgCl2 x 6 H2O 0.1 g/l Ca(NO3)2 x 4 H2O 0.05 g/l Na2-EDTA 0.0005 g/l H3BO3 0.0003 g/l FeSO4 x 7 H2O 0.0002 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
23276Nutrient agar (NA)yes
37643MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121324CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
3791positivegrowth30mesophilic
23276positivegrowth30mesophilic
23276positivegrowth37mesophilic
23276positivegrowth42thermophilic
23276nogrowth4psychrophilic
37643positivegrowth37mesophilic
59271positivegrowth30-37mesophilic
121324positivegrowth15-41
121324nogrowth5psychrophilic
121324nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59271aerobe
121324obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

halophily

@refsaltgrowthtested relationconcentration
121324NaClpositivegrowth0-4 %
121324NaClnogrowth6 %
121324NaClnogrowth8 %
121324NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2327625115malate-carbon source
23276911224-nitrophenyl alpha-D-glucopyranoside-hydrolysis
232763557154-nitrophenyl beta-D-galactopyranoside-hydrolysis
23276902594-nitrophenyl beta-D-glucopyranoside-hydrolysis
23276901464-nitrophenyl beta-D-glucuronide-hydrolysis
23276901484-nitrophenyl beta-D-xylopyranoside-hydrolysis
232763122bis-4-nitrophenyl phosphate-hydrolysis
2327691050bis-4-nitrophenyl-phenyl phosphonate-hydrolysis
2327655394bis-4-nitrophenyl-phosphorylcholine-hydrolysis
23276gamma-L-glutamate-4-nitroanilide-hydrolysis
23276L-aniline-4-nitroanilide-hydrolysis
23276L-proline-4-nitroanilide-hydrolysis
23276L-alanine 4-nitroanilide+hydrolysis
12132416947citrate-carbon source
1213244853esculin-hydrolysis
121324606565hippurate+hydrolysis
12132417632nitrate-builds gas from
12132417632nitrate-reduction
12132416301nitrite-builds gas from
12132416301nitrite-reduction
12132415792malonate-assimilation
12132417632nitrate-respiration

metabolite production

  • @ref: 121324
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12132415688acetoin-
12132417234glucose-

enzymes

@refvalueactivityec
23276cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121324oxidase+
121324beta-galactosidase+3.2.1.23
121324alcohol dehydrogenase-1.1.1.1
121324gelatinase-
121324amylase-
121324DNase-
121324caseinase-3.4.21.50
121324catalase+1.11.1.6
121324tween esterase-
121324gamma-glutamyltransferase-2.3.2.2
121324lecithinase-
121324lipase-
121324lysine decarboxylase-4.1.1.18
121324ornithine decarboxylase-4.1.1.17
121324phenylalanine ammonia-lyase-4.3.1.24
121324protease-
121324tryptophan deaminase-
121324urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121324--++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121324---------------------------------+-----------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3791water of river Elberiver ElbeGermanyDEUEurope
23276River Elbe in Germany after enrichment of a mixed bacterial culture with 6-aminonaphthalene-2-sulphonate
59271WaterRiver ElbeGermanyDEUEurope
121324Environment, Water, Elbe riverGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Engineered#Laboratory#Simulated communities
#Environmental#Aquatic#River (Creek)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4460.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_2229;97_2700;98_3352;99_4460&stattab=map
  • Last taxonomy: Pusillimonas
  • 16S sequence: AY695828
  • Sequence Identity:
  • Total samples: 530
  • soil counts: 207
  • aquatic counts: 172
  • animal counts: 135
  • plant counts: 16

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
37911Risk group (German classification)
1213241Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Proteobacteria 16S rRNA (BN9)X59160158ena2722
23276Pusillimonas noertemannii 16S ribosomal RNA gene, complete sequenceAY6958281525nuccore305977

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pusillimonas noertemannii DSM 10065GCA_003545825contigncbi305977
66792Pusillimonas noertemannii DSM 10065GCA_013416295contigncbi305977
66792Pusillimonas noertemannii DSM 10065GCA_003096595scaffoldncbi305977
66792Pusillimonas noertemannii strain DSM 10065305977.4wgspatric305977
66792Pusillimonas noertemannii strain DSM 10065305977.8wgspatric305977
66792Pusillimonas noertemannii strain DSM 10065305977.3wgspatric305977
66792Pusillimonas noertemannii DSM 100652756170261draftimg305977

GC content

  • @ref: 23276
  • GC-content: 61.8
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno55.388no
gram-positiveno97.391no
anaerobicno99.002no
halophileno86.635no
spore-formingno93.342no
glucose-utilno85.087no
aerobicyes87.027no
thermophileno98.394no
motileyes84.807no
glucose-fermentno91.482no

External links

@ref: 3791

culture collection no.: DSM 10065, CCUG 51657, NCIMB 14020, CIP 108767

straininfo link

  • @ref: 70042
  • straininfo: 48741

literature

  • topic: Phylogeny
  • Pubmed-ID: 15879236
  • title: Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates.
  • authors: Stolz A, Burger S, Kuhm A, Kampfer P, Busse HJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63466-0
  • year: 2005
  • mesh: Alcaligenaceae/*classification/cytology/isolation & purification/physiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA/genetics, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3791Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10065)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10065
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23276Andreas Stolz, Sibylle Bürger, Andrea Kuhm, Peter Kämpfer, Hans-Jürgen Busse10.1099/ijs.0.63466-0Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylatesIJSEM 55: 1077-1081 200515879236
37643Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6432
59271Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51657)https://www.ccug.se/strain?id=51657
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70042Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48741.1StrainInfo: A central database for resolving microbial strain identifiers
121324Curators of the CIPCollection of Institut Pasteur (CIP 108767)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108767