Strain identifier
BacDive ID: 357
Type strain:
Species: Pigmentiphaga kullae
Strain Designation: K24
Culture col. no.: CCUG 47266, CIP 108234, DSM 13608, NCIMB 13708
General
@ref: 5109
BacDive-ID: 357
DSM-Number: 13608
keywords: gram-negative, mesophilic, Bacteria, 16S sequence, genome sequence, motile, rod-shaped
description: Pigmentiphaga kullae K24 is a mesophilic, gram-negative, motile bacterium that was isolated from soil.
strain history: <- A. Stolz, Univ. Stuttgart <- S. Blümel <- T. Leisinger, ETH Zuerich; K24
doi: 10.13145/bacdive357.20201210.5
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/proteobacteria
- domain: Bacteria
- phylum: Proteobacteria
- class: Betaproteobacteria
- order: Burkholderiales
- family: Alcaligenaceae
- genus: Pigmentiphaga
- species: Pigmentiphaga kullae
- full scientific name: Pigmentiphaga kullae Blümel et al. 2001
@ref: 5109
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Alcaligenaceae
genus: Pigmentiphaga
species: Pigmentiphaga kullae
full scientific name: Pigmentiphaga kullae Blümel et al. 2001
strain designation: K24
type strain: yes
Morphology
cell morphology
- @ref: 23273
- gram stain: negative
- cell length: 1.3-4 µm
- cell width: 0.7-1.2 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
@ref | colony size | incubation period | medium used | colony color | colony shape |
---|---|---|---|---|---|
23273 | 1 mm | 3 days | LB agar | ||
23273 | opaque | circular |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5109 | REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830c.pdf | |
5109 | REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1a.pdf | |
23273 | LB (Luria-Bertani) MEDIUM | yes | ||
33215 | MEDIUM 6 - Columbia agar with 10 % horse blood | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml) |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5109 | positive | growth | 30 | mesophilic |
23273 | positive | growth | 30 | mesophilic |
23273 | positive | growth | 37 | mesophilic |
23273 | positive | growth | 42 | thermophilic |
23273 | no | growth | 4 | psychrophilic |
33215 | positive | growth | 30 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 23273
- spore formation: no
compound production
- @ref: 5109
- compound: azoreductase
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23273 | 15963 | ribitol | - | builds acid from |
23273 | 17057 | cellobiose | - | builds acid from |
23273 | 18333 | D-arabitol | - | builds acid from |
23273 | 16899 | D-mannitol | - | builds acid from |
23273 | 16024 | D-mannose | - | builds acid from |
23273 | 65327 | D-xylose | - | builds acid from |
23273 | 16813 | galactitol | - | builds acid from |
23273 | 17113 | erythritol | - | builds acid from |
23273 | 17234 | glucose | - | builds acid from |
23273 | 17268 | myo-inositol | - | builds acid from |
23273 | 30849 | L-arabinose | - | builds acid from |
23273 | 17716 | lactose | - | builds acid from |
23273 | 17306 | maltose | - | builds acid from |
23273 | 28053 | melibiose | - | builds acid from |
23273 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
23273 | 16634 | raffinose | - | builds acid from |
23273 | 26546 | rhamnose | - | builds acid from |
23273 | 17814 | salicin | - | builds acid from |
23273 | 30911 | sorbitol | - | builds acid from |
23273 | 17992 | sucrose | - | builds acid from |
23273 | 27082 | trehalose | - | builds acid from |
23273 | 16865 | gamma-aminobutyric acid | - | carbon source |
23273 | 15963 | ribitol | - | carbon source |
23273 | 18305 | arbutin | - | carbon source |
23273 | 17057 | cellobiose | - | carbon source |
23273 | 15824 | D-fructose | - | carbon source |
23273 | 12936 | D-galactose | - | carbon source |
23273 | 17634 | D-glucose | - | carbon source |
23273 | 17306 | maltose | - | carbon source |
23273 | 16899 | D-mannitol | - | carbon source |
23273 | 16024 | D-mannose | - | carbon source |
23273 | 28053 | melibiose | - | carbon source |
23273 | 16988 | D-ribose | - | carbon source |
23273 | 17924 | D-sorbitol | - | carbon source |
23273 | 16551 | D-trehalose | - | carbon source |
23273 | 65327 | D-xylose | - | carbon source |
23273 | 24265 | gluconate | - | carbon source |
23273 | 16977 | L-alanine | - | carbon source |
23273 | 30849 | L-arabinose | - | carbon source |
23273 | 15971 | L-histidine | - | carbon source |
23273 | 15603 | L-leucine | - | carbon source |
23273 | 15729 | L-ornithine | - | carbon source |
23273 | 17295 | L-phenylalanine | - | carbon source |
23273 | 62345 | L-rhamnose | - | carbon source |
23273 | 17115 | L-serine | - | carbon source |
23273 | 16828 | L-tryptophan | - | carbon source |
23273 | 68428 | maltitol | - | carbon source |
23273 | 17268 | myo-inositol | - | carbon source |
23273 | 28037 | N-acetylgalactosamine | - | carbon source |
23273 | 506227 | N-acetylglucosamine | - | carbon source |
23273 | 18401 | phenylacetate | - | carbon source |
23273 | 17148 | putrescine | - | carbon source |
23273 | 17814 | salicin | - | carbon source |
23273 | 17992 | sucrose | - | carbon source |
23273 | 91122 | 4-nitrophenyl alpha-D-glucopyranoside | - | hydrolysis |
23273 | 355715 | 4-nitrophenyl beta-D-galactopyranoside | - | hydrolysis |
23273 | 90259 | 4-nitrophenyl beta-D-glucopyranoside | - | hydrolysis |
23273 | 90146 | 4-nitrophenyl beta-D-glucuronide | - | hydrolysis |
23273 | 90148 | 4-nitrophenyl beta-D-xylopyranoside | - | hydrolysis |
23273 | 3122 | bis-4-nitrophenyl phosphate | - | hydrolysis |
23273 | 91050 | bis-4-nitrophenyl-phenyl phosphonate | - | hydrolysis |
23273 | 55394 | bis-4-nitrophenyl-phosphorylcholine | - | hydrolysis |
23273 | gamma-L-glutamate-4-nitroanilide | - | hydrolysis | |
23273 | L-aniline-4-nitroanilide | - | hydrolysis | |
23273 | L-proline-4-nitroanilide | - | hydrolysis | |
23273 | 30916 | 2-oxoglutarate | + | carbon source |
23273 | 16193 | 3-hydroxybenzoate | + | carbon source |
23273 | 37054 | 3-hydroxybutyrate | + | carbon source |
23273 | 17879 | 4-hydroxybenzoate | + | carbon source |
23273 | 30089 | acetate | + | carbon source |
23273 | 17128 | adipate | + | carbon source |
23273 | 78208 | azelaate | + | carbon source |
23273 | 16383 | cis-aconitate | + | carbon source |
23273 | 16947 | citrate | + | carbon source |
23273 | 29806 | fumarate | + | carbon source |
23273 | 17859 | glutaric acid | + | carbon source |
23273 | 17240 | itaconate | + | carbon source |
23273 | 29991 | L-aspartate | + | carbon source |
23273 | 24996 | lactate | + | carbon source |
23273 | 36986 | mesaconate | + | carbon source |
23273 | 17272 | propionate | + | carbon source |
23273 | 15361 | pyruvate | + | carbon source |
23273 | 9300 | suberic acid | + | carbon source |
23273 | 15708 | trans-aconitate | + | carbon source |
23273 | L-alanine 4-nitroanilide | + | hydrolysis |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23273 | catalase | + | 1.11.1.6 |
23273 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
5109 | soil | Switzerland | CHE | Europe | |
57611 | Soil,enrichment with 1-(4'-carboxy-phenylazo)-4-n. | Switzerland | CHE | Europe | Zürich ? |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 5109
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 23273
- description: Pigmentiphaga kullae strain K24 16S ribosomal RNA gene, partial sequence
- accession: AF282916
- length: 1482
- database: nuccore
- NCBI tax ID: 151784
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pigmentiphaga kullae K24 | GCA_004216695 | contig | ncbi | 151784 |
66792 | Pigmentiphaga kullae strain K24 | 151784.3 | wgs | patric | 151784 |
66792 | Pigmentiphaga kullae DSM 13608 | 2795386136 | permanent | img | 151784 |
External links
@ref: 5109
culture collection no.: CCUG 47266, CIP 108234, DSM 13608, NCIMB 13708
straininfo link
@ref | passport |
---|---|
20218 | http://www.straininfo.net/strains/163751 |
20218 | http://www.straininfo.net/strains/163752 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5109 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13608) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13608 | |||
20215 | D.Gleim, M.Kracht, N.Weiss et. al. | http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html | Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23273 | S Bl?mel, B Mark, H J Busse, P K?mpfer, A Stolz | 10.1099/00207713-51-5-1867 | Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically. | IJSEM 51: 1867-1871 2001 | ||
33215 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5834 | ||||
57611 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 47266) | https://www.ccug.se/strain?id=47266 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) |