Strain identifier

BacDive ID: 357

Type strain: Yes

Species: Pigmentiphaga kullae

Strain Designation: K24

Culture col. no.: CCUG 47266, CIP 108234, DSM 13608, NCIMB 13708

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General

@ref: 5109

BacDive-ID: 357

DSM-Number: 13608

keywords: gram-negative, mesophilic, Bacteria, 16S sequence, genome sequence, motile, rod-shaped

description: Pigmentiphaga kullae K24 is a mesophilic, gram-negative, motile bacterium that was isolated from soil.

strain history: <- A. Stolz, Univ. Stuttgart <- S. Blümel <- T. Leisinger, ETH Zuerich; K24

doi: 10.13145/bacdive357.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Pigmentiphaga
  • species: Pigmentiphaga kullae
  • full scientific name: Pigmentiphaga kullae Blümel et al. 2001

@ref: 5109

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Pigmentiphaga

species: Pigmentiphaga kullae

full scientific name: Pigmentiphaga kullae Blümel et al. 2001

strain designation: K24

type strain: yes

Morphology

cell morphology

  • @ref: 23273
  • gram stain: negative
  • cell length: 1.3-4 µm
  • cell width: 0.7-1.2 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony sizeincubation periodmedium usedcolony colorcolony shape
232731 mm3 daysLB agar
23273opaquecircular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5109REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830c.pdf
5109REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1a.pdf
23273LB (Luria-Bertani) MEDIUMyes
33215MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@refgrowthtypetemperaturerange
5109positivegrowth30mesophilic
23273positivegrowth30mesophilic
23273positivegrowth37mesophilic
23273positivegrowth42thermophilic
23273nogrowth4psychrophilic
33215positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 23273
  • spore formation: no

compound production

  • @ref: 5109
  • compound: azoreductase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2327315963ribitol-builds acid from
2327317057cellobiose-builds acid from
2327318333D-arabitol-builds acid from
2327316899D-mannitol-builds acid from
2327316024D-mannose-builds acid from
2327365327D-xylose-builds acid from
2327316813galactitol-builds acid from
2327317113erythritol-builds acid from
2327317234glucose-builds acid from
2327317268myo-inositol-builds acid from
2327330849L-arabinose-builds acid from
2327317716lactose-builds acid from
2327317306maltose-builds acid from
2327328053melibiose-builds acid from
23273320061methyl alpha-D-glucopyranoside-builds acid from
2327316634raffinose-builds acid from
2327326546rhamnose-builds acid from
2327317814salicin-builds acid from
2327330911sorbitol-builds acid from
2327317992sucrose-builds acid from
2327327082trehalose-builds acid from
2327316865gamma-aminobutyric acid-carbon source
2327315963ribitol-carbon source
2327318305arbutin-carbon source
2327317057cellobiose-carbon source
2327315824D-fructose-carbon source
2327312936D-galactose-carbon source
2327317634D-glucose-carbon source
2327317306maltose-carbon source
2327316899D-mannitol-carbon source
2327316024D-mannose-carbon source
2327328053melibiose-carbon source
2327316988D-ribose-carbon source
2327317924D-sorbitol-carbon source
2327316551D-trehalose-carbon source
2327365327D-xylose-carbon source
2327324265gluconate-carbon source
2327316977L-alanine-carbon source
2327330849L-arabinose-carbon source
2327315971L-histidine-carbon source
2327315603L-leucine-carbon source
2327315729L-ornithine-carbon source
2327317295L-phenylalanine-carbon source
2327362345L-rhamnose-carbon source
2327317115L-serine-carbon source
2327316828L-tryptophan-carbon source
2327368428maltitol-carbon source
2327317268myo-inositol-carbon source
2327328037N-acetylgalactosamine-carbon source
23273506227N-acetylglucosamine-carbon source
2327318401phenylacetate-carbon source
2327317148putrescine-carbon source
2327317814salicin-carbon source
2327317992sucrose-carbon source
23273911224-nitrophenyl alpha-D-glucopyranoside-hydrolysis
232733557154-nitrophenyl beta-D-galactopyranoside-hydrolysis
23273902594-nitrophenyl beta-D-glucopyranoside-hydrolysis
23273901464-nitrophenyl beta-D-glucuronide-hydrolysis
23273901484-nitrophenyl beta-D-xylopyranoside-hydrolysis
232733122bis-4-nitrophenyl phosphate-hydrolysis
2327391050bis-4-nitrophenyl-phenyl phosphonate-hydrolysis
2327355394bis-4-nitrophenyl-phosphorylcholine-hydrolysis
23273gamma-L-glutamate-4-nitroanilide-hydrolysis
23273L-aniline-4-nitroanilide-hydrolysis
23273L-proline-4-nitroanilide-hydrolysis
23273309162-oxoglutarate+carbon source
23273161933-hydroxybenzoate+carbon source
23273370543-hydroxybutyrate+carbon source
23273178794-hydroxybenzoate+carbon source
2327330089acetate+carbon source
2327317128adipate+carbon source
2327378208azelaate+carbon source
2327316383cis-aconitate+carbon source
2327316947citrate+carbon source
2327329806fumarate+carbon source
2327317859glutaric acid+carbon source
2327317240itaconate+carbon source
2327329991L-aspartate+carbon source
2327324996lactate+carbon source
2327336986mesaconate+carbon source
2327317272propionate+carbon source
2327315361pyruvate+carbon source
232739300suberic acid+carbon source
2327315708trans-aconitate+carbon source
23273L-alanine 4-nitroanilide+hydrolysis

enzymes

@refvalueactivityec
23273catalase+1.11.1.6
23273cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5109soilSwitzerlandCHEEurope
57611Soil,enrichment with 1-(4'-carboxy-phenylazo)-4-n.SwitzerlandCHEEuropeZürich ?

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 5109
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23273
  • description: Pigmentiphaga kullae strain K24 16S ribosomal RNA gene, partial sequence
  • accession: AF282916
  • length: 1482
  • database: nuccore
  • NCBI tax ID: 151784

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pigmentiphaga kullae K24GCA_004216695contigncbi151784
66792Pigmentiphaga kullae strain K24151784.3wgspatric151784
66792Pigmentiphaga kullae DSM 136082795386136permanentimg151784

External links

@ref: 5109

culture collection no.: CCUG 47266, CIP 108234, DSM 13608, NCIMB 13708

straininfo link

@refpassport
20218http://www.straininfo.net/strains/163751
20218http://www.straininfo.net/strains/163752

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5109Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13608)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13608
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23273S Bl?mel, B Mark, H J Busse, P K?mpfer, A Stolz10.1099/00207713-51-5-1867Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically.IJSEM 51: 1867-1871 2001
33215Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5834
57611Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47266)https://www.ccug.se/strain?id=47266
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)