Strain identifier

BacDive ID: 350

Type strain: Yes

Species: Castellaniella ginsengisoli

Strain history: <- DC Yang, Kyunghee Univ.

NCBI tax ID(s): 546114 (species)

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General

@ref: 17461

BacDive-ID: 350

DSM-Number: 23970

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Castellaniella ginsengisoli DSM 23970 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from soil of ginseng field.

NCBI tax id

  • NCBI tax id: 546114
  • Matching level: species

strain history

@refhistory
17461<- KCTC <- D.-C. Yang, Kyung Hee Univ., Seoul, Republic of Korea
67770D.-C. Yang DCY36.
67771<- DC Yang, Kyunghee Univ.

doi: 10.13145/bacdive350.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Castellaniella
  • species: Castellaniella ginsengisoli
  • full scientific name: Castellaniella ginsengisoli Kim et al. 2009

@ref: 17461

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Castellaniella

species: Castellaniella ginsengisoli

full scientific name: Castellaniella ginsengisoli Kim et al. 2009

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23269negative1.6-2.5 µm0.7-0.8 µmrod-shapedyes
67771negative

colony morphology

  • @ref: 23269
  • colony size: 1-2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17461R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23269LB (Luria-Bertani) MEDIUMyes
23269Nutrient agar (NA)yes
23269Reasoner's 2A agar (R2A)yes
23269Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
17461positivegrowth28mesophilic
23269positivegrowth25-42
23269positiveoptimum30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23269positivegrowth5.0-9.0alkaliphile
23269positiveoptimum6.5-7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

observation

  • @ref: 67771
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23269168082-dehydro-D-gluconate-assimilation
23269174265-dehydro-D-gluconate-assimilation
2326916988D-ribose-assimilation
2326917924D-sorbitol-assimilation
2326928087glycogen-assimilation
2326917268myo-inositol-assimilation
2326917240itaconate-assimilation
2326918287L-fucose-assimilation
2326915971L-histidine-assimilation
2326962345L-rhamnose-assimilation
2326915792malonate-assimilation
2326928053melibiose-assimilation
2326917814salicin-assimilation
232699300suberic acid-assimilation
2326917992sucrose-assimilation
232695291gelatin-hydrolysis
23269O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2326928017starch-hydrolysis
23269161933-hydroxybenzoate+assimilation
23269178794-hydroxybenzoate+assimilation
2326930089acetate+assimilation
2326917128adipate+assimilation
2326927689decanoate+assimilation
2326916947citrate+assimilation
2326917634D-glucose+assimilation
2326916899D-mannitol+assimilation
2326916024D-mannose+assimilation
23269370543-hydroxybutyrate+assimilation
2326924265gluconate+assimilation
2326916977L-alanine+assimilation
2326930849L-arabinose+assimilation
2326915589L-malate+assimilation
2326917203L-proline+assimilation
2326917115L-serine+assimilation
2326924996lactate+assimilation
2326917306maltose+assimilation
23269506227N-acetylglucosamine+assimilation
2326918401phenylacetate+assimilation
2326917272propionate+assimilation
2326931011valerate+assimilation
232694853esculin+hydrolysis
2326917632nitrate+reduction

enzymes

@refvalueactivityec
23269acid phosphatase+3.1.3.2
23269alkaline phosphatase+3.1.3.1
23269alpha-chymotrypsin-3.4.21.1
23269alpha-galactosidase-3.2.1.22
23269alpha-glucosidase-3.2.1.20
23269alpha-mannosidase-3.2.1.24
23269arginine dihydrolase-3.5.3.6
23269beta-galactosidase-3.2.1.23
23269beta-glucosidase+3.2.1.21
23269beta-glucosidase-3.2.1.21
23269beta-glucuronidase-3.2.1.31
23269catalase+1.11.1.6
23269cystine arylamidase-3.4.11.3
23269cytochrome oxidase+1.9.3.1
23269esterase (C 4)+
23269esterase lipase (C 8)+
23269leucine arylamidase+3.4.11.1
23269lipase (C 14)-
23269N-acetyl-beta-glucosaminidase-3.2.1.52
23269naphthol-AS-BI-phosphohydrolase+
23269trypsin-3.4.21.4
23269urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17461soil of ginseng fieldDaejeonRepublic of KoreaKORAsia
67770Soil of a ginseng field in DaejeonRepublic of KoreaKORAsia
67771From soil of a ginseng field in South KoreaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 17461
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17461
  • description: Castellaniella ginsengisoli strain DCY36 16S ribosomal RNA gene, partial sequence
  • accession: EU873313
  • length: 1441
  • database: ena
  • NCBI tax ID: 546114

GC content

@refGC-contentmethod
1746163.71
6777063.7high performance liquid chromatography (HPLC)

External links

@ref: 17461

culture collection no.: DSM 23970, JCM 15515, KCTC 22398, DCY 36

straininfo link

  • @ref: 70030
  • straininfo: 400840

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19605728Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.Kim MK, Srinivasan S, Kim YJ, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.008300-02009Alcaligenaceae/*classification/enzymology/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Korea, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Panax, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analysis, beta-Glucosidase/*biosynthesisEnzymology
Phylogeny19819992Castellaniella daejeonensis sp. nov., isolated from soil.Lee M, Jung HM, Woo SG, Yoo SA, Ten LNInt J Syst Evol Microbiol10.1099/ijs.0.018887-02009Alcaligenaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyMetabolism
Phylogeny22523161Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.Glaeser SP, Galatis H, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.041392-02012Alcaligenaceae/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Leeches/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Putrescine/analogs & derivatives/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysisGenetics
Pathogenicity26352961FATAL SUPPURATIVE INFLAMMATION IN DAURIAN PIKAS (OCHOTONA DAUURICA) NATURALLY INFECTED WITH CASTELLANIELLA GINSENGISOLI-LIKE BACTERIA.Thompson R, Gebhart CJ, Armien AG, Olsen K, Marthaler D, Rasmussen JM, Wolf TM, Wunschmann AJ Zoo Wildl Med10.1638/2015-0029.12015Alcaligenaceae/*isolation & purification, Animals, Animals, Zoo, Female, Gram-Negative Bacterial Infections/microbiology/mortality/*veterinary, *Lagomorpha, Male, Soft Tissue Infections/microbiology/mortality/*veterinaryEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17461Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23970)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23970
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23269Myung Kyum Kim, Sathiyaraj Srinivasan, Yeon-Ju Kim, Deok-Chun Yang10.1099/ijs.0.008300-0Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacteriumIJSEM 59: 2191-2194 200919605728
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
70030Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400840.1StrainInfo: A central database for resolving microbial strain identifiers