Strain identifier
BacDive ID: 348
Type strain:
Species: Castellaniella denitrificans
Strain Designation: NKNTAU
Strain history: CIP <- 2006, CCUG <- 1998, K. Denger, Konstanz Univ., Konstanz, Germany: strain NKNTAU
NCBI tax ID(s): 56119 (species)
General
@ref: 4168
BacDive-ID: 348
DSM-Number: 11046
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Castellaniella denitrificans NKNTAU is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from anaerobic sewage digester.
NCBI tax id
- NCBI tax id: 56119
- Matching level: species
strain history
@ref | history |
---|---|
4168 | <- K. Denger, Universität Konstanz, Fakultät für Biologie, Konstanz, Germany; NKNTAU (Alcaligenes sp.) <- K. Denger {1996} |
118893 | CIP <- 2006, CCUG <- 1998, K. Denger, Konstanz Univ., Konstanz, Germany: strain NKNTAU |
doi: 10.13145/bacdive348.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Alcaligenaceae
- genus: Castellaniella
- species: Castellaniella denitrificans
- full scientific name: Castellaniella denitrificans Kämpfer et al. 2006
@ref: 4168
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Alcaligenaceae
genus: Castellaniella
species: Castellaniella denitrificans
full scientific name: Castellaniella denitrificans Kämpfer et al. 2006
strain designation: NKNTAU
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
31661 | negative | 1.3-2 µm | 0.2-0.8 µm | rod-shaped | yes |
118893 | negative | rod-shaped | yes |
colony morphology
- @ref: 118893
pigmentation
- @ref: 31661
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4168 | ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503) | yes | https://mediadive.dsmz.de/medium/503 | Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: NaNO3 1.69492 g/l Na2CO3 1.49551 g/l Taurine 1.24626 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water |
37484 | MEDIUM 328- for nutrient agar | yes | Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g) | |
118893 | CIP Medium 328 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4168 | positive | growth | 30 | mesophilic |
31661 | positive | growth | 25-42 | |
31661 | positive | optimum | 25-30 | mesophilic |
37484 | positive | growth | 30 | mesophilic |
54834 | positive | growth | 30-37 | mesophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
31661 | positive | growth | 7 |
31661 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
4168 | anaerobe |
31661 | facultative anaerobe |
54834 | aerobe |
118893 | obligate aerobe |
spore formation
- @ref: 31661
- spore formation: no
observation
- @ref: 31661
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31661 | 30089 | acetate | + | carbon source |
31661 | 16449 | alanine | + | carbon source |
31661 | 35391 | aspartate | + | carbon source |
31661 | 24265 | gluconate | + | carbon source |
31661 | 24996 | lactate | + | carbon source |
31661 | 25017 | leucine | + | carbon source |
31661 | 25115 | malate | + | carbon source |
31661 | 18257 | ornithine | + | carbon source |
31661 | 26271 | proline | + | carbon source |
31661 | 17272 | propionate | + | carbon source |
31661 | 15361 | pyruvate | + | carbon source |
31661 | 17822 | serine | + | carbon source |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
118893 | 17632 | nitrate | + | reduction |
118893 | 16301 | nitrite | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
118893 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
118893 | oxidase | + | |
118893 | catalase | + | 1.11.1.6 |
118893 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
54834 | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
118893 | - | + | + | - | - | + | - | - | - | - | - | - | + | - | - | - | + | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
54834 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
4168 | anaerobic sewage digester | Germany | DEU | Europe |
54834 | Anaerobic sewage digester | Germany | DEU | Europe |
118893 | Environment, Anaerobic sewage digester | Germany | DEU | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | #Anaerobic digestor |
#Engineered | #Waste | #Wastewater |
#Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_17735.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_739;97_862;98_1006;99_17735&stattab=map
- Last taxonomy: Castellaniella denitrificans
- 16S sequence: U82826
- Sequence Identity:
- Total samples: 6820
- soil counts: 1695
- aquatic counts: 3384
- animal counts: 1188
- plant counts: 553
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4168 | 1 | Risk group (German classification) |
118893 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 4168
- description: Castellaniella denitrificans strain NKNTAU 16S ribosomal RNA gene, complete sequence
- accession: U82826
- length: 1470
- database: ena
- NCBI tax ID: 56119
External links
@ref: 4168
culture collection no.: CIP 109221, DSM 11046, CCUG 39541
straininfo link
- @ref: 70028
- straininfo: 359321
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 16585701 | Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov. | Kampfer P, Denger K, Cook AM, Lee ST, Jackel U, Denner EBM, Busse HJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.63989-0 | 2006 | Alcaligenaceae/*classification/isolation & purification, Alcaligenes/*classification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/analysis/genetics | Genetics |
Phylogeny | 18768620 | Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant. | Liu QM, Ten LN, Im WT, Lee ST | Int J Syst Evol Microbiol | 10.1099/ijs.0.65314-0 | 2008 | Alcaligenaceae/*classification/genetics/*isolation & purification/physiology, Anaerobiosis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Industrial Microbiology, Locomotion, Molecular Sequence Data, Nitrogen/metabolism, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Ubiquinone/analysis | Genetics |
Phylogeny | 19605728 | Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium. | Kim MK, Srinivasan S, Kim YJ, Yang DC | Int J Syst Evol Microbiol | 10.1099/ijs.0.008300-0 | 2009 | Alcaligenaceae/*classification/enzymology/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Korea, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Panax, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analysis, beta-Glucosidase/*biosynthesis | Enzymology |
Phylogeny | 19819992 | Castellaniella daejeonensis sp. nov., isolated from soil. | Lee M, Jung HM, Woo SG, Yoo SA, Ten LN | Int J Syst Evol Microbiol | 10.1099/ijs.0.018887-0 | 2009 | Alcaligenaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology | Metabolism |
Phylogeny | 22523161 | Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana. | Glaeser SP, Galatis H, Martin K, Kampfer P | Int J Syst Evol Microbiol | 10.1099/ijs.0.041392-0 | 2012 | Alcaligenaceae/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Leeches/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Putrescine/analogs & derivatives/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis | Genetics |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
4168 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11046) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11046 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31661 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27942 | 28776041 | |
37484 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6938 | ||||
54834 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 39541) | https://www.ccug.se/strain?id=39541 | |||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
70028 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID359321.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118893 | Curators of the CIP | Collection of Institut Pasteur (CIP 109221) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109221 |