Strain identifier

BacDive ID: 339

Type strain: Yes

Species: Azohydromonas lata

Strain Designation: H-4, H4

Strain history: CIP <- 1991, CCUG <- ATCC <- M.L. Palleroni: strain H4

NCBI tax ID(s): 1314749 (strain), 45677 (species)

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General

@ref: 566

BacDive-ID: 339

DSM-Number: 1122

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile

description: Azohydromonas lata H-4 is a mesophilic, motile bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
1314749strain
45677species

strain history

@refhistory
566<- N.J. Palleroni, H-4
67770IAM 12599 <-- ATCC 29712 <-- N. J. Palleroni H-4.
120565CIP <- 1991, CCUG <- ATCC <- M.L. Palleroni: strain H4

doi: 10.13145/bacdive339.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Azohydromonas
  • species: Azohydromonas lata
  • full scientific name: Azohydromonas lata (Palleroni and Palleroni 1978) Xie and Yokota 2005

  • synonyms

    • @ref: 20215
    • synonym: Alcaligenes latus

@ref: 566

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Azohydromonas

species: Azohydromonas lata

full scientific name: Azohydromonas lata (Palleroni and Palleroni 1978) Xie and Yokota 2005

strain designation: H-4, H4

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes92.596
6948099.998negative
120565yesnegativerod-shaped

colony morphology

  • @ref: 120565

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38132MEDIUM 12 - for Alcaligenes latus and Rhodobacter capsulatusyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (3.000 g);Peptone (6.000 g)
566MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)yesName: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled waterhttps://mediadive.dsmz.de/medium/81
566NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
120565CIP Medium 12yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=12

culture temp

@refgrowthtypetemperaturerange
38132positivegrowth30mesophilic
566positivegrowth30mesophilic
67770positivegrowth30mesophilic
120565positivegrowth15-37
120565nogrowth10psychrophilic
120565nogrowth41thermophilic
120565nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.764

compound production

  • @ref: 566
  • compound: PHB

halophily

@refsaltgrowthtested relationconcentration
120565NaClpositivegrowth0 %
120565NaClnogrowth2 %
120565NaClnogrowth4 %
120565NaClnogrowth6 %
120565NaClnogrowth8 %
120565NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12056516947citrate-carbon source
1205654853esculin-hydrolysis
120565606565hippurate-hydrolysis
12056517632nitrate+builds gas from
12056517632nitrate+reduction
12056516301nitrite+builds gas from
12056516301nitrite-reduction
12056515792malonate-assimilation
12056517632nitrate-respiration

antibiotic resistance

  • @ref: 120565
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120565
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12056515688acetoin-
12056517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120565oxidase+
120565beta-galactosidase-3.2.1.23
120565alcohol dehydrogenase-1.1.1.1
120565gelatinase-
120565amylase+
120565catalase+1.11.1.6
120565gamma-glutamyltransferase+2.3.2.2
120565lysine decarboxylase-4.1.1.18
120565ornithine decarboxylase-4.1.1.17
120565phenylalanine ammonia-lyase+4.3.1.24
120565tryptophan deaminase-
120565urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120565-+++-+----++---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
566soilCaliforniaUSAUSANorth America
67770SoilCAUSAUSANorth America
120565Environment, SoilBerkeley, CaliforniaUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
5661Risk group (German classification)
1205651Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Azohydromonas lata gene for 16S rRNA, partial sequence, strain:IAM 12599AB1881251462ena45677
20218Alcaligenes latus DNA for 16S ribosomal RNAD880071467ena45677
20218Azohydromonas lata gene for 16S rRNA, partial sequence, strain: NBRC 102462AB6818041454ena45677

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Azohydromonas lata NBRC 1024621314749.5wgspatric1314749
66792Azohydromonas lata NBRC 1024622731957616draftimg1314749
67770Azohydromonas lata NBRC 102462GCA_001571085contigncbi1314749
66792Azohydromonas lata NBRC 102462 MsE-30jul19-236GCA_945878385contigncbi1314749
66792Azohydromonas lata NBRC 102462 strain MsE-30jul19-2361314749.8wgspatric1314749

GC content

  • @ref: 566
  • GC-content: 69.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes85.87no
gram-positiveno98.936no
anaerobicno95.148no
halophileno94.844no
spore-formingno92.995no
thermophileno98.412yes
glucose-utilyes71.155no
flagellatedyes70.529no
aerobicyes82.046no
glucose-fermentno90.23no

External links

@ref: 566

culture collection no.: DSM 1122, ATCC 29712, IAM 12599, LMG 3321, JCM 20675, CCUG 7831, CIP 103458, ICPB 4234, LMG 3323, LMG 3326, NBRC 102462, NCIMB 12188, CCUG 10983

straininfo link

  • @ref: 70019
  • straininfo: 801

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism12018290Production of specific copolymers of polyhydroxyalkanoates from industrial waste.Wong PA, Chua H, Lo W, Lawford HG, Yu PHAppl Biochem Biotechnol10.1385/abab:98-100:1-9:6552002Alcaligenes/metabolism, Biodegradation, Environmental, Butyric Acid/chemistry, Calorimetry, Differential Scanning, Carboxylic Acids/*chemistry, Cupriavidus necator/growth & development/metabolism, Fermentation, Food Analysis, Industrial Waste/*analysis, Klebsiella/growth & development/metabolism, Pentanoic Acids/chemistry, Plastics, Staphylococcus/growth & development/metabolismBiotechnology
Phylogeny16280506Reclassification of Alcaligenes latus strains IAM 12599T and IAM 12664 and Pseudomonas saccharophila as Azohydromonas lata gen. nov., comb. nov., Azohydromonas australica sp. nov. and Pelomonas saccharophila gen. nov., comb. nov., respectively.Xie CH, Yokota AInt J Syst Evol Microbiol10.1099/ijs.0.63733-02005Alcaligenes/*classification/genetics/isolation & purification/physiology, Base Composition, Comamonadaceae/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Molecular Sequence Data, Nitrogen Fixation, Nucleic Acid Hybridization, Phenazines, Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/genetics, Sequence Analysis, DNAEnzymology
Phylogeny28124782Azohydromonas riparia sp. nov. and Azohydromonas ureilytica sp. nov. isolated from a riverside soil in South Korea.Nguyen TM, Kim JJ Microbiol10.1007/s12275-017-6519-z2017Alcaligenaceae/chemistry/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/*genetics, DNA, Ribosomal, Fatty Acids/analysis, Phosphatidylethanolamines, Phylogeny, RNA, Ribosomal, 16S, Republic of Korea, Sequence Analysis, DNA, *Soil MicrobiologyTranscriptome
Phylogeny34093463Azohydromonas caseinilytica sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method.Dahal RH, Chaudhary DK, Kim DU, Kim JFront Microbiol10.3389/fmicb.2021.6471322021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
566Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1122)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1122
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38132Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15258
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70019Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID801.1StrainInfo: A central database for resolving microbial strain identifiers
120565Curators of the CIPCollection of Institut Pasteur (CIP 103458)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103458