Strain identifier

BacDive ID: 33

Type strain: Yes

Species: Acidomonas methanolica

Strain Designation: MB 58

Strain history: CIP <- 1988, IMET <- Inst. Biotech. Akad. Wissenchaften, Leipzig, Germany: strain MB58, Acetobacter methanoliticus

NCBI tax ID(s): 1231351 (strain), 437 (species)

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General

@ref: 2164

BacDive-ID: 33

DSM-Number: 5432

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Acidomonas methanolica MB 58 is a mesophilic bacterium that was isolated from yeast fermentation process.

NCBI tax id

NCBI tax idMatching level
1231351strain
437species

strain history

@refhistory
2164<- IMET <- K. Karbaum, MB 58
67770T. Urakami TK 0705 <-- IMET 10945 <-- K. Karbaum MB58.
120923CIP <- 1988, IMET <- Inst. Biotech. Akad. Wissenchaften, Leipzig, Germany: strain MB58, Acetobacter methanoliticus

doi: 10.13145/bacdive33.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acidomonas
  • species: Acidomonas methanolica
  • full scientific name: Acidomonas methanolica (Uhlig et al. 1986) Urakami et al. 1989

  • synonyms

    • @ref: 20215
    • synonym: Acetobacter methanolicus

@ref: 2164

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acidomonas

species: Acidomonas methanolica

full scientific name: Acidomonas methanolica (Uhlig et al. 1986) Urakami et al. 1989 emend. Yamashita et al. 2004

strain designation: MB 58

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2164SABOURAND GLUCOSE MEDIUM (DSMZ Medium 1429)yeshttps://mediadive.dsmz.de/medium/1429Name: SABOURAUD GLUCOSE MEDIUM (DSMZ Medium 1429) Composition: SABOURAUD- Glucose-Bouillon 30.0 g/l Agar 15.0 g/l Distilled water
2164MEDIUM 125 WITH METHANOL (DSMZ Medium 569)yeshttps://mediadive.dsmz.de/medium/569Name: MEDIUM 125 WITH METHANOL (DSMZ Medium 569) Composition: Agar 12.0 g/l Methanol 7.92 g/l KNO3 1.0 g/l Na2HPO4 0.23 g/l MgSO4 x 7 H2O 0.2 g/l NaH2PO4 0.07 g/l CaCl2 x 2 H2O 0.02 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 7e-05 g/l MoO3 1e-05 g/l MnSO4 x 5 H2O 1e-05 g/l H3BO3 1e-05 g/l CuSO4 x 5 H2O 5e-06 g/l Distilled water
40546MEDIUM 2 - for Acidomonas methanolicayesDistilled water make up to (1000.000 ml);Manganese II sulphate monohydrate(0.050 g);Maltose (5.000 g);Magnesium sulphate heptahydrate (0.200 g);Agar (20.000 g);Glucose (15.000 g);Yeast extract (1.000 g);Tween 80 (1.000 ml);Proteose Peptone N°3 (8.000 g);C
120923CIP Medium 2yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=2

culture temp

@refgrowthtypetemperaturerange
2164positivegrowth30mesophilic
40546positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: Q-10

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
2164yeast fermentation processLeipzigGermanyDEUEurope
53589Fermentation of methanol with Candida sp.LeipzigGermanyDEUEurope1980
67770Septic methanol-yeast process
120923Other, Septic methanol-yeast processGermanyDEUEurope

isolation source categories

Cat1Cat2
#Engineered#Biodegradation
#Host#Yeast

taxonmaps

  • @ref: 69479
  • File name: preview.99_3185.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_661;97_1985;98_2441;99_3185&stattab=map
  • Last taxonomy: Acidomonas methanolica subclade
  • 16S sequence: X77468
  • Sequence Identity:
  • Total samples: 10057
  • soil counts: 3564
  • aquatic counts: 2020
  • animal counts: 3308
  • plant counts: 1165

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
21641Risk group (German classification)
1209231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acidomonas methanolica gene for 16S ribosomal RNAD307701411ena437
20218Acidomonas methanolica 16S rRNA, partial sequenceAB003976155ena437
20218A.methanolicus 16S rRNA geneX774681481ena437
20218Acidomonas methanolica gene for 16S rRNA, complete sequence, strain: NRIC0498AB1107021409ena437

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidomonas methanolica NBRC 104435GCA_007988925contigncbi1231351
66792Acidomonas methanolica NBRC 1044351231351.7wgspatric1231351
66792Acidomonas methanolica NBRC 1044351231351.3wgspatric1231351
66792Acidomonas methanolica strain DSM 5432437.4wgspatric437
66792Acidomonas methanolica NBRC 1044352887682946draftimg1231351
66792Acidomonas methanolica DSM 54322784746772draftimg437
67770Acidomonas methanolica NBRC 104435 MB58GCA_000617865scaffoldncbi1231351
67770Acidomonas methanolica DSM 5432GCA_004346035contigncbi437

GC content

@refGC-contentmethod
6777062high performance liquid chromatography (HPLC)
6777062.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes69.402no
gram-positiveno96.428no
anaerobicno98.946no
halophileno94.012no
spore-formingno93.641no
thermophileno96.142yes
glucose-utilyes82.47no
flagellatedno87.966no
aerobicyes84.015no
glucose-fermentno89.233no

External links

@ref: 2164

culture collection no.: DSM 5432, ATCC 43581, IMET 10945, CCUG 37300, LMG 1668, JCM 6891, BCC 12263, BCRC 16049, CCM 4121, CIP 103167, NBRC 104435, NRIC 0498, VKM B-2104

straininfo link

@refstraininfo
6971792077
69718386976

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1811855Structure of the capsular polysaccharide and the O-side-chain of the lipopolysaccharide from Acetobacter methanolicus MB 58/4 (IMET 10945), and of oligosaccharides resulting from their degradation by the bacteriophage Acml.Grimmecke HD, Mamat U, Lauk W, Shashkov AS, Knirel YA, Vinogradov EV, Kochetkov NKCarbohydr Res10.1016/0008-6215(91)80014-e1991Acetobacter/*chemistry, Bacterial Capsules/*chemistry, Bacteriophages/*metabolism, Carbohydrate Sequence, Lipopolysaccharides/*chemistry/metabolism, Molecular Sequence Data, Oligosaccharides/chemistry, Polysaccharides, Bacterial/*chemistry/metabolismGenetics
2166787Mutagenesis of acetobacter methanolicus MB58 with the transposon Tn5.Dobrowolski P, Grundig MWJ Basic Microbiol10.1002/jobm.36203004171990Acetobacter/*genetics, *DNA Transposable Elements, *Mutation
Metabolism7512445Structure of the capsular polysaccharide and the O-side-chain of the lipopolysaccharide from Acetobacter methanolicus MB 70, and of oligosaccharides resulting from their degradation by the bacteriophage Acm6.Grimmecke HD, Knirel YA, Shashkov AS, Kiesel B, Lauk W, Voges MCarbohydr Res10.1016/0008-6215(94)80072-31994Acetobacter/*chemistry/metabolism, Bacteriophages/*metabolism, Carbohydrate Conformation, Carbohydrate Sequence, Chromatography, Gel, Chromatography, Ion Exchange, Galactose/analysis, Glucose/analysis, Lipopolysaccharides/*chemistry/isolation & purification/metabolism, Magnetic Resonance Spectroscopy, Methylation, Molecular Sequence Data, O Antigens, Oligosaccharides/*chemistry/isolation & purification, Polysaccharides, Bacterial/*chemistry/isolation & purification/metabolismEnzymology
Metabolism8376955Phage Acm1-mediated transduction in the facultatively methanol-utilizing Acetobacter methanolicus MB 58/4.Kiesel B, Wunsche LJ Gen Virol10.1099/0022-1317-74-9-17411993Acetobacter/*genetics/metabolism, Bacteriophages/*genetics/metabolism/ultrastructure, Methanol/metabolism, Microscopy, Electron, Mutagenesis, *Transduction, Genetic
Metabolism9143455The toxicity of substituted phenolic compounds to a detoxifying and an acetic acid bacterium.Loffhagen N, Hartig C, Babel WEcotoxicol Environ Saf10.1006/eesa.1996.15161997Acinetobacter/*drug effects/metabolism, Adenosine Diphosphate/metabolism, Adenosine Triphosphate/biosynthesis, Biodegradation, Environmental, Electron Transport/drug effects, Ethanol/metabolism, Luminescent Measurements, Oxidation-Reduction, Oxygen Consumption/drug effects, Phenols/chemistry/*toxicity, Photobacterium/metabolismPathogenicity
Phylogeny15143037Emendation of the genus Acidomonas Urakami, Tamaoka, Suzuki and Komagata 1989.Yamashita SI, Uchimura T, Komagata KInt J Syst Evol Microbiol10.1099/ijs.0.02946-02004Acetobacteraceae/*classification/genetics/isolation & purification/metabolism, Base Composition, Base Sequence, Carbon/metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Hydrogen-Ion Concentration, Methanol/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, TokyoMetabolism
Metabolism17095990Formaldehyde uptake by Methylobacterium sp. MF1 and Acidomonas methanolica MB 58 with the different formaldehyde assimilation pathways.Mitsui R, Kitazawa H, Sato T, Tanaka MEnviron Sci2006Formaldehyde/*metabolism, Methanol/metabolism, Methylobacteriaceae/*classification/*metabolism, Methylobacterium/*classification/*metabolismPhylogeny
Metabolism28515381Preliminary Evaluation of Glyceric Acid-producing Ability of Acidomonas methanolica NBRC104435 from Glycerol Containing Methanol.Sato S, Kitamoto D, Habe HJ Oleo Sci10.5650/jos.ess162362017Acetobacteraceae/growth & development/*metabolism, Glyceric Acids/*metabolism, Glycerol/chemistry/*metabolism, Methanol/chemistry/*metabolism, Stereoisomerism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2164Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5432)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5432
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40546Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14935
53589Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37300)https://www.ccug.se/strain?id=37300
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69717Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92077.1StrainInfo: A central database for resolving microbial strain identifiers
69718Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID386976.1StrainInfo: A central database for resolving microbial strain identifiers
120923Curators of the CIPCollection of Institut Pasteur (CIP 103167)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103167