Strain identifier

BacDive ID: 3223

Type strain: Yes

Species: Corynebacterium marinum

Strain Designation: 7015, D7015

Strain history: CIP <- 2010, NRRL

NCBI tax ID(s): 1224162 (strain), 349751 (species)

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General

@ref: 12206

BacDive-ID: 3223

DSM-Number: 44953

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium marinum 7015 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from coastal sediment close to a coal-fired power station.

NCBI tax id

NCBI tax idMatching level
349751species
1224162strain

strain history

@refhistory
347162010, NRRL
67770CGMCC 1.6998 <-- Z.-J. Du et al. D7015.
120810CIP <- 2010, NRRL

doi: 10.13145/bacdive3223.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium marinum
  • full scientific name: Corynebacterium marinum Du et al. 2010

@ref: 12206

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium marinum

full scientific name: Corynebacterium marinum Du et al. 2010 emend. Nouioui et al. 2018

strain designation: 7015, D7015

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
23203positiverod-shapedno
69480no96.856
69480positive100
120810positiveoval-shapedno

colony morphology

@refcolony colormedium usedcolony sizecolony shapeincubation period
69368Golden yellow (1004)ISP 2
69368Golden yellow (1004)ISP 6
23203yellowMA medium0.5-1.5 mmcircular2 days
69368Light ivory (1015)ISP 7
69368Light ivory (1015), golden yellow (1004)suter with tyrosine
69368Light ivory (1015), golden yellow (1004)suter without tyrosine
69368Oyster white (1013)ISP 3
69368Oyster white (1013)ISP 4
69368Oyster white (1013)ISP 5

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69368noAerial myceliumISP 2
69368noAerial myceliumISP 3
69368noAerial myceliumISP 4
69368noAerial myceliumISP 5
69368noAerial myceliumISP 6
69368noAerial myceliumISP 7
69368noAerial myceliumsuter with tyrosine
69368noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
69368noMelanin
69368nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
69368DSM_44953_image3.jpegPlates (535, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69368DSM_44953_image4.jpegPlates (535, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12206COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
12206TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23203MA mediumyes
34716Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120810CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
12206positivegrowth28mesophilic
23203positivegrowth4-37
23203positiveoptimum30-32mesophilic
34716positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23203facultative anaerobe
120810obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.997

halophily

@refsaltgrowthtested relationconcentration
23203NaClpositivegrowth0-8 %(w/v)
23203NaClpositiveoptimum1 %(w/v)
69368NaClpositivegrowth0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6936822599arabinose-growth
2320317716lactose-builds acid from
2320329864mannitol-builds acid from
2320333942ribose-builds acid from
2320318222xylose-builds acid from
2320317234glucose-fermentation
232034853esculin-hydrolysis
2320316199urea-hydrolysis
2320317234glucose+builds acid from
2320328087glycogen+builds acid from
2320317306maltose+builds acid from
2320317992sucrose+builds acid from
2320327941pullulan+hydrolysis
2320328017starch+hydrolysis
2320353424tween 20+hydrolysis
2320353423tween 40+hydrolysis
2320353425tween 60+hydrolysis
2320353426tween 80+hydrolysis
23203casein+other
2320316301nitrite+reduction
6936862968cellulose-growth
6936828757fructose+growth
6936817234glucose+growth
6936817268inositol-growth
6936837684mannose-growth
6936816634raffinose-growth
6936826546rhamnose-growth
6936817992sucrose-growth
6936818222xylose-growth
12081017632nitrate+reduction
12081016301nitrite-reduction
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2320327902tetracyclineyesyes100 µg (disc)
232039331sulfamethizoleyesyes200 µg (disc)
232033770co-trimoxazoleyesyes25 µg (disc)
23203100147nalidixic acidyesyes30 µg (disc)
2320371415nitrofurantoinyesyes50 µg (disc)
2320317833gentamicinyesyes10 µg (disc)
232033393carbenicillinyesyes100 µg (disc)
2320328971ampicillinyesyes25 µg (disc)
2320317076streptomycinyesyes25 µg (disc)
232036104kanamycinyesyes30 µg (disc)
2320317698chloramphenicolyesyes50 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2320315688acetoinyes
12081035581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
2320315688acetoin+
2320317234glucose-

enzymes

@refvalueactivityec
23203acid phosphatase-3.1.3.2
23203alkaline phosphatase-3.1.3.1
23203alpha-chymotrypsin+3.4.21.1
23203alpha-fucosidase-3.2.1.51
23203alpha-galactosidase-3.2.1.22
23203alpha-glucosidase-3.2.1.20
23203alpha-mannosidase-3.2.1.24
23203beta-galactosidase-3.2.1.23
23203beta-glucosidase-3.2.1.21
23203beta-glucuronidase+3.2.1.31
23203catalase+1.11.1.6
23203cystine arylamidase-3.4.11.3
23203cytochrome oxidase-1.9.3.1
23203esterase lipase (C 8)+
23203esterase lipase (C 8)-
23203leucine arylamidase+3.4.11.1
23203lipase (C 14)-
23203N-acetyl-beta-glucosaminidase-3.2.1.52
23203naphthol-AS-BI-phosphohydrolase+
23203pyrazinamidase+3.5.1.B15
23203pyrrolidonyl arylamidase-3.4.19.3
23203trypsin-3.4.21.4
23203valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
120810oxidase-
120810catalase+1.11.1.6
120810urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69368++--+--------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
69368-+/-+/--++/-----+/---+-+---
120810+++-+-----+--+-+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
12206coastal sediment close to a coal-fired power stationQingdaoChinaCHNAsia
67770Coastal sediment close to a coal-fired power stationQingdaoChinaCHNAsia
120810Environment, Coastal sedimentQuingdaoChinaCHNAsia2000

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_15884.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2122;97_2654;98_3291;99_15884&stattab=map
  • Last taxonomy: Corynebacterium marinum subclade
  • 16S sequence: DQ219354
  • Sequence Identity:
  • Total samples: 14123
  • soil counts: 2357
  • aquatic counts: 2122
  • animal counts: 8968
  • plant counts: 676

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
122061Risk group (German classification)
1208102Risk group (French classification)

Sequence information

16S sequences

  • @ref: 12206
  • description: Corynebacterium marinum DSM 44953 16S ribosomal RNA gene, partial sequence
  • accession: DQ219354
  • length: 1444
  • database: ena
  • NCBI tax ID: 1224162

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium marinum DSM 44953GCA_000835165completencbi1224162
66792Corynebacterium marinum CGMCC 1.6998GCA_014645275contigncbi349751
66792Corynebacterium marinum DSM 449531224162.11plasmidpatric1224162
66792Corynebacterium marinum DSM 449531224162.3completepatric1224162
66792Corynebacterium marinum DSM 449531224162.12plasmidpatric1224162
66792Corynebacterium marinum strain CGMCC 1.6998349751.5wgspatric349751
66792Corynebacterium marinum DSM 44953 Genome sequencing2630968455completeimg1224162

GC content

@refGC-contentmethod
1220665.0high performance liquid chromatography (HPLC)
6777065high performance liquid chromatography (HPLC)
6777067.8genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.062yes
flagellatedno98.009no
gram-positiveyes94.083yes
anaerobicno99.328yes
aerobicyes71.975yes
halophileyes86.197no
spore-formingno91.097no
glucose-utilyes88.223yes
thermophileno98.987no
glucose-fermentno53.366yes

External links

@ref: 12206

culture collection no.: DSM 44953, CGMCC 1.6998, NRRL B-24779, JCM 17491, CIP 110199

straininfo link

  • @ref: 72737
  • straininfo: 370387

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19783605Corynebacterium marinum sp. nov. isolated from coastal sediment.Du ZJ, Jordan EM, Rooney AP, Chen GJ, Austin BInt J Syst Evol Microbiol10.1099/ijs.0.018523-02009Corynebacterium/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny30688628Corynebacterium alimapuense sp. nov., an obligate marine actinomycete isolated from sediment of Valparaiso bay, Chile.Claverias F, Gonzales-Siles L, Salva-Serra F, Inganas E, Molin K, Cumsille A, Undabarrena A, Couve E, Moore ERB, Tindall BJ, Gomila M, Camara BInt J Syst Evol Microbiol10.1099/ijsem.0.0032372019Bacterial Typing Techniques, Base Composition, Bays, Chile, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12206Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44953)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44953
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23203Zong-Jun Du, Elizabeth M. Jordan, Alejandro P. Rooney, Guan-Jun Chen, Brian Austin10.1099/ijs.0.018523-0Corynebacterium marinum sp. nov. isolated from coastal sedimentIJSEM 60: 1944-1947 201019783605
34716Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8032
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69368Wink, J.https://cdn.dsmz.de/wink/DSM%2044953.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72737Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370387.1StrainInfo: A central database for resolving microbial strain identifiers
120810Curators of the CIPCollection of Institut Pasteur (CIP 110199)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110199