Strain identifier

BacDive ID: 322

Type strain: Yes

Species: Achromobacter insolitus

Strain history: CIP <- 2003, CCUG <- 2002, LMG <- D. Monget, Api, La Balme-les-Grottes, France

NCBI tax ID(s): 217204 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17377

BacDive-ID: 322

DSM-Number: 23807

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped, human pathogen

description: Achromobacter insolitus DSM 23807 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from human wound, leg.

NCBI tax id

  • NCBI tax id: 217204
  • Matching level: species

strain history

@refhistory
17377<- CCUG
120136CIP <- 2003, CCUG <- 2002, LMG <- D. Monget, Api, La Balme-les-Grottes, France

doi: 10.13145/bacdive322.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Achromobacter
  • species: Achromobacter insolitus
  • full scientific name: Achromobacter insolitus Coenye et al. 2003

@ref: 17377

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Achromobacter

species: Achromobacter insolitus

full scientific name: Achromobacter insolitus Coenye et al. 2003

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
23266negative1-2 µmcoccus-shaped
120136negativerod-shapedyes

colony morphology

@reftype of hemolysiscolony color
23266gammawhite to light brown
120136

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17377NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36483MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120136CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328
120136CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17377positivegrowth28mesophilic
23266positivegrowth28-37mesophilic
36483positivegrowth37mesophilic
57486positivegrowth30-37mesophilic
120136positivegrowth15-37
120136nogrowth5psychrophilic
120136nogrowth41thermophilic
120136nogrowth45thermophilic

Physiology and metabolism

tolerance

  • @ref: 23266
  • compound: Acetamide

oxygen tolerance

@refoxygen tolerance
57486aerobe
120136obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
23266NaClpositivemaximum4.5 %
120136NaClpositivegrowth0-6 %
120136NaClnogrowth8 %
120136NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2326632382(R)-mandelate-growth
2326632800(S)-mandelic acid-growth
23266168082-dehydro-D-gluconate-growth
23266161933-hydroxybenzoate-growth
23266178794-hydroxybenzoate-growth
23266391504-oxopentanoate-growth
23266158875-aminovaleric acid-growth
23266174265-dehydro-D-gluconate-growth
2326627856acetamide-growth
2326615963ribitol-growth
2326627613amygdalin-growth
2326618305arbutin-growth
2326640538benzylamine-growth
2326617750betaine-growth
2326643799butan-1-amine-growth
2326625646octanoate-growth
2326617057cellobiose-growth
2326616919creatine-growth
2326617108D-arabinose-growth
2326618333D-arabitol-growth
2326615824D-fructose-growth
2326628847D-fucose-growth
2326617634D-glucose-growth
2326662318D-lyxose-growth
2326616024D-mannose-growth
232666731melezitose-growth
2326616443D-tagatose-growth
2326630927D-tartrate-growth
2326632528turanose-growth
2326665327D-xylose-growth
2326616813galactitol-growth
2326617113erythritol-growth
232664853esculin-growth
2326616000ethanolamine-growth
2326615862ethylamine-growth
2326628757fructose-growth
2326628260galactose-growth
2326628066gentiobiose-growth
232665417glucosamine-growth
2326617234glucose-growth
2326617754glycerol-growth
2326628087glycogen-growth
2326629805glycolate-growth
2326618295histamine-growth
2326617268myo-inositol-growth
2326615443inulin-growth
2326630803isophthalate-growth
2326630849L-arabinose-growth
2326618403L-arabitol-growth
2326616467L-arginine-growth
2326618287L-fucose-growth
2326662345L-rhamnose-growth
2326617266L-sorbose-growth
2326665328L-xylose-growth
2326617716lactose-growth
2326615792malonate-growth
2326617306maltose-growth
2326629864mannitol-growth
2326628053melibiose-growth
23266320061methyl alpha-D-glucopyranoside-growth
2326643943methyl alpha-D-mannoside-growth
2326674863methyl beta-D-xylopyranoside-growth
23266506227N-acetylglucosamine-growth
2326630623oxalate-growth
2326674848pentan-1-amine-growth
2326617563phthalate-growth
2326616634raffinose-growth
2326633942ribose-growth
2326617814salicin-growth
2326615611sarcosine-growth
2326630911sorbitol-growth
2326615746spermine-growth
2326628017starch-growth
2326617992sucrose-growth
2326630043terephthalate-growth
2326627082trehalose-growth
2326618123trigonelline-growth
2326653426tween 80-growth
2326616199urea-growth
2326617151xylitol-growth
2326618222xylose-growth
232664853esculin-hydrolysis
2326616301nitrite-reduction
2326629991L-aspartate+growth
23266283402-aminobutyrate+growth
23266194752-aminopentanoic acid+growth
23266168082-dehydro-D-gluconate+growth
23266879973-aminobutyrate+growth
23266161933-hydroxybenzoate+growth
2326616865gamma-aminobutyric acid+growth
2326630089acetate+growth
2326622210aconitate+growth
2326617128adipate+growth
2326678208azelaate+growth
2326616958beta-alanine+growth
2326617968butyrate+growth
2326627689decanoate+growth
2326616947citrate+growth
2326615570D-alanine+growth
23266370543-hydroxybutyrate+growth
2326633871glycerate+growth
2326629806fumarate+growth
2326624265gluconate+growth
2326617859glutaric acid+growth
2326648944isobutyrate+growth
2326648942isovalerate+growth
2326617240itaconate+growth
2326616977L-alanine+growth
2326617561L-cysteine+growth
2326629985L-glutamate+growth
2326615971L-histidine+growth
2326617191L-isoleucine+growth
2326615603L-leucine+growth
2326618019L-lysine+growth
2326618347L-norleucine+growth
2326617295L-phenylalanine+growth
2326617203L-proline+growth
2326617115L-serine+growth
2326630924L-tartrate+growth
2326616857L-threonine+growth
2326616828L-tryptophan+growth
2326617895L-tyrosine+growth
2326616414L-valine+growth
2326624996lactate+growth
2326625115malate+growth
2326636986mesaconate+growth
2326630928meso-tartrate+growth
2326630860methylmalonic acid+growth
2326632361nonanoate+growth
2326618401phenylacetate+growth
2326617774pimelate+growth
2326617272propionate+growth
2326617148putrescine+growth
2326615361pyruvate+growth
2326641865sebacic acid+growth
232669300suberic acid+growth
2326630031succinate+growth
23266trimethoxybenzoate+growth
2326616765tryptamine+growth
2326631011valerate+growth
2326617632nitrate+reduction
12013616947citrate+carbon source
1201364853esculin-hydrolysis
120136606565hippurate+hydrolysis
12013617632nitrate-builds gas from
12013617632nitrate+reduction
12013616301nitrite-builds gas from
12013616301nitrite-reduction
12013615792malonate+assimilation
12013617632nitrate+respiration

antibiotic resistance

  • @ref: 120136
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
2326635581indoleno
2326616136hydrogen sulfideno
12013635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12013615688acetoin-
12013617234glucose-

enzymes

@refvalueactivityec
23266alpha-chymotrypsin-3.4.21.1
23266alpha-fucosidase-3.2.1.51
23266alpha-galactosidase-3.2.1.22
23266alpha-glucosidase-3.2.1.20
23266alpha-mannosidase-3.2.1.24
23266amylase-
23266arginine dihydrolase-3.5.3.6
23266beta-galactosidase-3.2.1.23
23266beta-glucoronidase-3.2.1.31
23266beta-glucosidase-3.2.1.21
23266catalase+1.11.1.6
23266cystine arylamidase-3.4.11.3
23266cytochrome oxidase+1.9.3.1
23266DNase-
23266esterase lipase (C 8)-
23266gelatinase-
23266leucine arylamidase+3.4.11.1
23266lipase (C 14)-
23266lysine decarboxylase-4.1.1.18
23266N-acetyl-beta-glucosaminidase-3.2.1.52
23266ornithine decarboxylase-4.1.1.17
23266trypsin-3.4.21.4
23266urease-3.5.1.5
23266valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120136oxidase+
120136beta-galactosidase-3.2.1.23
120136alcohol dehydrogenase-1.1.1.1
120136gelatinase-
120136amylase-
120136DNase-
120136caseinase-3.4.21.50
120136catalase+1.11.1.6
120136tween esterase-
120136gamma-glutamyltransferase+2.3.2.2
120136lecithinase-
120136lipase-
120136lysine decarboxylase+4.1.1.18
120136ornithine decarboxylase-4.1.1.17
120136phenylalanine ammonia-lyase-4.3.1.24
120136tryptophan deaminase-
120136urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120136--+--+----+---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120136-------------------------------------------++--+++++++------++---++------+-+++-++++--+-+++++++--+++

Isolation, sampling and environmental information

isolation

@refsample typesampling dateisolation date
17377human wound, leg
57486Human wound,leg1984
120136Human, Wound1984

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Wound
#Host Body-Site#Limb#Leg

taxonmaps

  • @ref: 69479
  • File name: preview.99_954.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_9;97_9;98_570;99_954&stattab=map
  • Last taxonomy: Achromobacter
  • 16S sequence: JQ746423
  • Sequence Identity:
  • Total samples: 7288
  • soil counts: 1429
  • aquatic counts: 1212
  • animal counts: 2896
  • plant counts: 1751

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
17377yes, in single cases1Risk group (German classification)
1201361Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Achromobacter insolitus strain CCUG 47057 16S ribosomal RNA gene, partial sequenceJQ7464231309ena217204
17377Achromobacter sp. LMG 6003 16S ribosomal RNA gene, partial sequenceAY1708471473ena217204

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Achromobacter insolitus DSM 23807GCA_001971645completencbi217204
66792Achromobacter insolitus LMG 6003GCA_002209555contigncbi217204
66792Achromobacter insolitus LMG 6003GCA_902859615contigncbi217204
66792Achromobacter insolitus strain DSM 23807217204.3completepatric217204
66792Achromobacter insolitus strain LMG 6003217204.5wgspatric217204
66792Achromobacter insolitus strain LMG 6003217204.13wgspatric217204
66792Achromobacter insolitus DSM 238072751185845completeimg217204
66792Achromobacter insolitus LMG 60032855745187draftimg217204

GC content

@refGC-contentmethod
1737764.9
2326664.9high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno55.867no
gram-positiveno98.316yes
anaerobicno98.417no
aerobicyes88.582no
halophileno89.062no
spore-formingno94.215no
glucose-utilno59.621no
thermophileno99.533no
motileyes87.376no
glucose-fermentno90.871no

External links

@ref: 17377

culture collection no.: DSM 23807, CCUG 47057, LMG 6003, CIP 108202, API 201-3-84, Gilardi 3038

straininfo link

  • @ref: 70003
  • straininfo: 793

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14657110Achromobacter insolitus sp. nov. and Achromobacter spanius sp. nov., from human clinical samples.Coenye T, Vancanneyt M, Falsen E, Swings J, Vandamme PInt J Syst Evol Microbiol10.1099/ijs.0.02698-02003Achromobacter/*classification/genetics/isolation & purification, DNA, Ribosomal/genetics, Gram-Negative Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/geneticsPathogenicity
Phylogeny19749028Pusillimonas ginsengisoli sp. nov., isolated from soil of a ginseng field.Srinivasan S, Kim MK, Sathiyaraj G, Kim YJ, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.018358-02009Alcaligenaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Panax/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyMetabolism
Phylogeny27902206Achromobacter aloeverae sp. nov., isolated from the root of Aloe vera (L.) Burm.f.Kuncharoen N, Muramatsu Y, Shibata C, Kamakura Y, Nakagawa Y, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0015662017Achromobacter/*classification/genetics/isolation & purification, Aloe/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Ubiquinone/chemistryTranscriptome
Metabolism28369432Complete genome sequence of Achromobacter insolitus type strain LMG 6003T, a pathogen isolated from leg wound.Li G, Zhang T, Yang L, Cao Y, Guo X, Qin J, Yang Q, You S, Yuan G, Jiang K, Luo J, Li Z, Gao L, Jiang K, Wu L, Zheng JPathog Dis10.1093/femspd/ftx0372017Achromobacter/classification/*genetics/isolation & purification, Base Composition, *Chromosome Mapping, DNA, Bacterial/*genetics/metabolism, DNA, Circular/genetics/metabolism, Endopeptidases/genetics/metabolism, Fimbriae Proteins/genetics/metabolism, *Gene Expression Regulation, Bacterial, Gene Ontology, *Genome, Bacterial, Genomic Islands, Humans, Leg/microbiology, Molecular Sequence Annotation, RNA, Ribosomal, 16S/genetics/metabolism, RNA, Transfer/genetics/metabolism, Type VI Secretion Systems/genetics/metabolism, Whole Genome SequencingGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17377Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23807)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23807
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23266Tom Coenye, Marc Vancanneyt, Enevold Falsen, Jean Swings, Peter Vandamme10.1099/ijs.0.02698-0Achromobacter insolitus sp. nov. and Achromobacter spanius sp. nov., from human clinical samplesIJSEM 53: 1819-1824 200314657110
36483Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5799
57486Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47057)https://www.ccug.se/strain?id=47057
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70003Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID793.1StrainInfo: A central database for resolving microbial strain identifiers
120136Curators of the CIPCollection of Institut Pasteur (CIP 108202)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108202