Strain identifier
BacDive ID: 3206
Type strain:
Species: Corynebacterium testudinoris
Strain Designation: M935/96/4
Strain history: CIP <- 2000, CCUG <- MD Collins, Reading, UK: strain M935/96/4 <- G. Foster, Inverness, UK
NCBI tax ID(s): 136857 (species)
General
@ref: 11927
BacDive-ID: 3206
DSM-Number: 44614
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, pigmented, animal pathogen
description: Corynebacterium testudinoris M935/96/4 is a facultative anaerobe, mesophilic, Gram-positive animal pathogen that has a yellow pigmentation and was isolated from from necrotic lesions in the mouth of a tortoise.
NCBI tax id
- NCBI tax id: 136857
- Matching level: species
strain history
@ref | history |
---|---|
11927 | <- CCUG <- M. D. Collins, IFR Reading <- G. Foster |
67770 | CCUG 41823 <-- M. D. Collins <-- G. Foster M935/96/4. |
123487 | CIP <- 2000, CCUG <- MD Collins, Reading, UK: strain M935/96/4 <- G. Foster, Inverness, UK |
doi: 10.13145/bacdive3206.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Corynebacteriaceae
- genus: Corynebacterium
- species: Corynebacterium testudinoris
- full scientific name: Corynebacterium testudinoris Collins et al. 2001
@ref: 11927
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Corynebacteriaceae
genus: Corynebacterium
species: Corynebacterium testudinoris
full scientific name: Corynebacterium testudinoris Collins et al. 2001 emend. Nouioui et al. 2018
strain designation: M935/96/4
type strain: yes
Morphology
cell morphology
@ref | gram stain | motility | confidence | cell shape |
---|---|---|---|---|
23371 | positive | no | ||
69480 | no | 90.862 | ||
69480 | positive | 100 | ||
123487 | positive | no | rod-shaped |
colony morphology
@ref | medium used |
---|---|
23371 | Columbia blood agar base supplemented with 5% defibrinated sheep blood |
123487 |
pigmentation
- @ref: 23371
- production: yes
- color: yellow
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11927 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
23371 | Columbia blood agar base supplemented with 5% defibrinated sheep blood | yes | ||
40595 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
123487 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11927 | positive | growth | 37 | mesophilic |
23371 | positive | growth | 37 | mesophilic |
40595 | positive | growth | 37 | mesophilic |
55225 | positive | growth | 25-37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
123487 | positive | growth | 10-37 | |
123487 | no | growth | 41 | thermophilic |
123487 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23371 | facultative anaerobe |
55225 | aerobe |
123487 | facultative anaerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
23371 | no | |
69481 | no | 100 |
69480 | no | 99.997 |
halophily
- @ref: 123487
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-10 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23371 | 65327 | D-xylose | - | builds acid from |
23371 | 28087 | glycogen | - | builds acid from |
23371 | 17716 | lactose | - | builds acid from |
23371 | 29864 | mannitol | - | builds acid from |
23371 | 5291 | gelatin | - | hydrolysis |
23371 | 17234 | glucose | + | builds acid from |
23371 | 17306 | maltose | + | builds acid from |
23371 | 33942 | ribose | + | builds acid from |
23371 | 17992 | sucrose | + | builds acid from |
23371 | 4853 | esculin | + | hydrolysis |
23371 | 17632 | nitrate | + | reduction |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | - | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | - | builds acid from |
68371 | 28066 | gentiobiose | + | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | - | builds acid from |
68371 | 28017 | starch | - | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | + | builds acid from |
68371 | 17992 | sucrose | + | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | - | builds acid from |
68371 | 17306 | maltose | + | builds acid from |
68371 | 17814 | salicin | + | builds acid from |
68371 | 4853 | esculin | + | builds acid from |
68371 | 18305 | arbutin | + | builds acid from |
68371 | 27613 | amygdalin | + | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | + | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 17634 | D-glucose | + | builds acid from |
68371 | 12936 | D-galactose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | + | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
123487 | 16947 | citrate | - | carbon source |
123487 | 4853 | esculin | + | hydrolysis |
123487 | 606565 | hippurate | - | hydrolysis |
123487 | 17632 | nitrate | + | reduction |
123487 | 16301 | nitrite | - | reduction |
123487 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 123487
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 123487
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
123487 | 15688 | acetoin | - | |
123487 | 17234 | glucose | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23371 | acid phosphatase | + | 3.1.3.2 |
23371 | alkaline phosphatase | - | 3.1.3.1 |
23371 | alpha-fucosidase | - | 3.2.1.51 |
23371 | alpha-galactosidase | - | 3.2.1.22 |
23371 | alpha-glucosidase | - | 3.2.1.20 |
23371 | alpha-mannosidase | - | 3.2.1.24 |
23371 | beta-galactosidase | - | 3.2.1.23 |
23371 | beta-glucosidase | + | 3.2.1.21 |
23371 | beta-glucuronidase | - | 3.2.1.31 |
23371 | catalase | + | 1.11.1.6 |
23371 | chymotrypsin | - | 3.4.4.5 |
23371 | cystine arylamidase | - | 3.4.11.3 |
23371 | esterase (C 4) | + | |
23371 | esterase lipase (C 8) | + | |
23371 | leucine arylamidase | + | 3.4.11.1 |
23371 | lipase (C 14) | - | |
23371 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
23371 | phosphoamidase | - | 3.9.1.1 |
23371 | pyrazinamidase | - | 3.5.1.B15 |
23371 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
23371 | trypsin | - | 3.4.21.4 |
23371 | urease | - | 3.5.1.5 |
23371 | valine arylamidase | - | |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
123487 | oxidase | - | |
123487 | beta-galactosidase | - | 3.2.1.23 |
123487 | alcohol dehydrogenase | - | 1.1.1.1 |
123487 | gelatinase | - | |
123487 | DNase | - | |
123487 | caseinase | - | 3.4.21.50 |
123487 | catalase | + | 1.11.1.6 |
123487 | tween esterase | - | |
123487 | gamma-glutamyltransferase | + | 2.3.2.2 |
123487 | lecithinase | - | |
123487 | lipase | - | |
123487 | lysine decarboxylase | - | 4.1.1.18 |
123487 | ornithine decarboxylase | - | 4.1.1.17 |
123487 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
123487 | tryptophan deaminase | - | |
123487 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123487 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | + | + | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123487 | - | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | +/- | + | + | + | + | +/- | + | - | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123487 | + | + | - | + | + | - | - | + | - | + | + | - | - | - | - | - | + | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | + | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
11927 | from necrotic lesions in the mouth of a tortoise | Inverness | United Kingdom | GBR | Europe | |
55225 | Tortoise,necrotic lesions in mouth | Inverness | United Kingdom | GBR | Europe | 1996 |
67770 | Necrotic lesions in the mouth of a tortoise | United Kingdom | GBR | Europe | ||
123487 | Tortoise, necrotic lesions in mouth | Inverness | United Kingdom | GBR | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Inflammation | |
#Host | #Reptilia | |
#Host Body-Site | #Oral cavity and airways | #Mouth |
#Host Body-Site | #Other | #Wound |
Safety information
risk assessment
@ref | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|
11927 | yes, in single cases | 1 | Risk group (German classification) |
123487 | 2 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 11927
- description: Corynebacterium testudinoris 16S rRNA gene, strain CCUG 41823T
- accession: AJ295841
- length: 1482
- database: ena
- NCBI tax ID: 136857
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Corynebacterium testudinoris DSM 44614 | GCA_001021045 | complete | ncbi | 136857 |
66792 | Corynebacterium testudinoris strain DSM 44614 | 136857.5 | complete | patric | 136857 |
66792 | Corynebacterium testudinoris DSM 44614 Genome Sequencing | 2615840659 | complete | img | 136857 |
GC content
- @ref: 67770
- GC-content: 63.14
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 92.786 | no |
flagellated | no | 97.889 | yes |
gram-positive | yes | 92.74 | no |
anaerobic | no | 99.378 | yes |
aerobic | no | 52.326 | no |
halophile | yes | 64.56 | no |
spore-forming | no | 94.391 | yes |
thermophile | no | 98.743 | no |
glucose-util | yes | 88.569 | no |
glucose-ferment | yes | 59.133 | no |
External links
@ref: 11927
culture collection no.: DSM 44614, ATCC BAA 467, CCUG 41823, CIP 106763, JCM 12108, NCIMB 14414
straininfo link
- @ref: 72721
- straininfo: 87799
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 11491332 | Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. | Collins MD, Hoyles L, Hutson RA, Foster G, Falsen E | Int J Syst Evol Microbiol | 10.1099/00207713-51-4-1349 | 2001 | Animals, Animals, Wild/microbiology, Cats/*microbiology, Corynebacterium/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Scotland, Species Specificity, Terminology as Topic, Turtles/*microbiology | Genetics |
Phylogeny | 25404484 | Corynebacterium atrinae sp. nov., isolated from the gastrointestinal tract of a pen shell, Atrina pectinata. | Kim PS, Shin NR, Hyun DW, Kim JY, Whon TW, Oh SJ, Bae JW | Int J Syst Evol Microbiol | 10.1099/ijs.0.067587-0 | 2014 | Animals, Bacterial Typing Techniques, Base Composition, Bivalvia/*microbiology, Corynebacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization/genetics, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Genetics | 26227591 | Complete Genome Sequence of the Type Strain Corynebacterium testudinoris DSM 44614, Recovered from Necrotic Lesions in the Mouth of a Tortoise. | Ruckert C, Kriete M, Jaenicke S, Winkler A, Tauch A | Genome Announc | 10.1128/genomeA.00784-15 | 2015 | ||
Phylogeny | 30688628 | Corynebacterium alimapuense sp. nov., an obligate marine actinomycete isolated from sediment of Valparaiso bay, Chile. | Claverias F, Gonzales-Siles L, Salva-Serra F, Inganas E, Molin K, Cumsille A, Undabarrena A, Couve E, Moore ERB, Tindall BJ, Gomila M, Camara B | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003237 | 2019 | Bacterial Typing Techniques, Base Composition, Bays, Chile, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11927 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44614) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44614 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23371 | M D Collins, L Hoyles, R A Hutson, G Foster, E Falsen | 10.1099/00207713-51-4-1349 | Corynebacterium testudinoris sp. nov., from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. | IJSEM 51: 1349-1352 2001 | 11491332 | |
40595 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18929 | ||||
55225 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 41823) | https://www.ccug.se/strain?id=41823 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
72721 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID87799.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123487 | Curators of the CIP | Collection of Institut Pasteur (CIP 106763) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106763 |