Strain identifier

BacDive ID: 3201

Type strain: Yes

Species: Corynebacterium auriscanis

Strain Designation: M598/96/3

Strain history: CIP <- 2000, CCUG <- 1998, M. D. Collins, IFR, Reading, UK <- L. Hoyles, Reading, UK <- G. Foster, Inverness, UK

NCBI tax ID(s): 99807 (species)

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General

@ref: 11922

BacDive-ID: 3201

DSM-Number: 44609

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive

description: Corynebacterium auriscanis M598/96/3 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from dog ear infection, bilateral otitis.

NCBI tax id

  • NCBI tax id: 99807
  • Matching level: species

strain history

@refhistory
11922<- CCUG <- M. D. Collins, IFR Reading <- L. Hoyles <- G. Foster, Inverness, UK
67770CCUG 39938 <-- M. D. Collins M598/96/1 <-- L. Hoyles <-- G. Foster.
116639CIP <- 2000, CCUG <- 1998, M. D. Collins, IFR, Reading, UK <- L. Hoyles, Reading, UK <- G. Foster, Inverness, UK

doi: 10.13145/bacdive3201.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium auriscanis
  • full scientific name: Corynebacterium auriscanis Collins et al. 2000

@ref: 11922

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium auriscanis

full scientific name: Corynebacterium auriscanis Collins et al. 2000 emend. Nouioui et al. 2018

strain designation: M598/96/3

type strain: yes

Morphology

cell morphology

@refgram stainconfidencemotility
69480positive99.999
6948095.931no
116639positiveno

colony morphology

  • @ref: 116639

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
39550MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116639CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
39550positivegrowth37mesophilic
67770positivegrowth37mesophilic
116639positivegrowth15-41
116639nogrowth10psychrophilic
116639nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116639
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.984

halophily

  • @ref: 116639
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116639citrate-carbon source16947
116639esculin-hydrolysis4853
116639hippurate+hydrolysis606565
116639nitrate-reduction17632
116639nitrite-reduction16301
116639nitrate-respiration17632

antibiotic resistance

  • @ref: 116639
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116639
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11663915688acetoin-
11663917234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116639oxidase-
116639beta-galactosidase-3.2.1.23
116639alcohol dehydrogenase-1.1.1.1
116639gelatinase-
116639amylase-
116639DNase+
116639caseinase-3.4.21.50
116639catalase+1.11.1.6
116639tween esterase+
116639gamma-glutamyltransferase-2.3.2.2
116639lecithinase-
116639lipase-
116639lysine decarboxylase-4.1.1.18
116639ornithine decarboxylase-4.1.1.17
116639phenylalanine ammonia-lyase-4.3.1.24
116639protease-
116639tryptophan deaminase-
116639urease-3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    54999C14:03.814
    54999C16:037.716
    54999C16:1 ω11c415.757
    54999C18:1 ω9c49.917.769
    54999C18:2 ω6,9c/C18:0 ANTE4.617.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    54999C14:02.714
    54999C15:04.515
    54999C16:037.216
    54999C17:02.117
    54999C18:0418
    54999C16:1 ω7c1.115.819
    54999C16:1 ω9c0.615.774
    54999C17:0 anteiso5.116.722
    54999C17:1 ω9c2.516.773
    54999C18:1 ω9c24.717.769
    54999C18:2 ω6,9c/C18:0 ANTE15.517.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116639-+++++--------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116639----+/-----+/-+/---------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116639+-+----------------+-----------------------------+---------------------------+-++-------++++--+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
11922dog ear infection, bilateral otitisUnited KingdomGBREurope
54999Dog ear infection,bilateral otitisUnited KingdomGBREurope1996
67770Ear of a dog suffering from bilateral otitis
116639Animal, Dog, otitisUnited KingdomGBREurope1996

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Organ#Ear

taxonmaps

  • @ref: 69479
  • File name: preview.99_6953.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1684;97_2017;98_2481;99_6953&stattab=map
  • Last taxonomy: Corynebacterium auriscanis
  • 16S sequence: AB595944
  • Sequence Identity:
  • Total samples: 845
  • soil counts: 6
  • aquatic counts: 20
  • animal counts: 819

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119222Risk group (German classification)
1166392Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium auriscanis 16S ribosomal RNA gene, partial sequenceAY438049418ena99807
67770Corynebacterium auriscanis gene for 16S rRNA, partial sequence, strain: JCM 12369AB5959441460ena99807

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium auriscanis CIP 10662999807.3wgspatric99807
66792Corynebacterium auriscanis CIP 1066292627854278draftimg99807
67770Corynebacterium auriscanis CIP 106629GCA_000767255contigncbi99807

GC content

@refGC-contentmethod
1192261
6777058.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno94.797no
flagellatedno97.78no
gram-positiveyes92.855no
anaerobicno99.246no
halophileyes65.83no
spore-formingno93.077no
thermophileno93.452yes
glucose-utilyes87.385no
aerobicno78.952no
glucose-fermentno66.638no

External links

@ref: 11922

culture collection no.: JCM 12369, DSM 44609, CCUG 39938, CIP 106629, LMG 19530

straininfo link

  • @ref: 72717
  • straininfo: 265695

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10523531Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov.Collins MD, Hoyles L, Lawson PA, Falsen E, Robson RL, Foster GJ Clin Microbiol10.1128/JCM.37.11.3443-3447.19991999Animals, Corynebacterium/*classification/*genetics/isolation & purification, Dog Diseases/microbiology, Dogs/*microbiology, Molecular Sequence Data, Otitis/microbiology/veterinary, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species SpecificityGenetics
Genetics27516502Whole-Genome Sequence of Corynebacterium auriscanis Strain CIP 106629 Isolated from a Dog with Bilateral Otitis from the United Kingdom.Tiwari S, Jamal SB, Oliveira LC, Clermont D, Bizet C, Mariano D, de Carvalho PV, Souza F, Pereira FL, de Castro Soares S, Guimaraes LC, Dorella F, Carvalho A, Leal C, Barh D, Figueiredo H, Hassan SS, Azevedo V, Silva AGenome Announc10.1128/genomeA.00683-162016Phylogeny
Phylogeny34846289Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces.Zhang G, Yang J, Lai XH, Jin D, Lu S, Ren Z, Qin T, Pu J, Ge Y, Cheng Y, Yang C, Lv X, Jiao Y, Huang Y, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0050692021Animals, Bacterial Typing Techniques, Base Composition, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Humans, *Marmota/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Trachea/microbiology, Vitamin K 2/analogs & derivatives/chemistryPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11922Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44609)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44609
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39550Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18781
54999Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39938)https://www.ccug.se/strain?id=39938
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72717Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265695.1StrainInfo: A central database for resolving microbial strain identifiers
116639Curators of the CIPCollection of Institut Pasteur (CIP 106629)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106629