Strain identifier

BacDive ID: 3196

Type strain: Yes

Species: Corynebacterium glaucum

Strain history: CIP <- 2003, DSMZ <- A.F. Yassin, Bonn Univ., Bonn, Germany: strain IMMIB R-5091

NCBI tax ID(s): 187491 (species)

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General

@ref: 11736

BacDive-ID: 3196

DSM-Number: 44530

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, dumbbell-shaped

description: Corynebacterium glaucum DSM 44530 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from clinical material .

NCBI tax id

  • NCBI tax id: 187491
  • Matching level: species

strain history

@refhistory
11736<- A. F. Yassin, Univ. Bonn; IMMIB R-5091
67770DSM 44530 <-- A. F. Yassin IMMIB R-5091.
119640CIP <- 2003, DSMZ <- A.F. Yassin, Bonn Univ., Bonn, Germany: strain IMMIB R-5091

doi: 10.13145/bacdive3196.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium glaucum
  • full scientific name: Corynebacterium glaucum Yassin et al. 2003

@ref: 11736

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium glaucum

full scientific name: Corynebacterium glaucum Yassin et al. 2003

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23186positivedumbbell-shapedno
119640positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
20068Beige (1001)10-14 daysISP 2
20068Beige (1001)10-14 daysISP 3
20068Beige (1001)10-14 daysISP 4
20068Beige (1001)10-14 daysISP 5
20068Beige (1001)10-14 daysISP 6
20068Beige (1001)10-14 daysISP 7
23186light-greyColumbia blood agar supplemented with 5 % sheep blood, BHI agar and trypticase soy agar
119640

multicellular morphology

@refforms multicellular complexmedium name
20068noISP 2
20068noISP 3
20068noISP 4
20068noISP 5
20068noISP 6
20068noISP 7

multimedia

  • @ref: 11736
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44530.jpg
  • caption: Medium 693 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11736COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
11736BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
20068ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20068ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20068ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20068ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20068ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20068ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
23186Columbia blood agar supplemented with 5 % sheep blood, BHI agar and trypticase soy agaryes
38249MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119640CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
20068positiveoptimum28mesophilic
11736positivegrowth37mesophilic
38249positivegrowth37mesophilic
58074positivegrowth37mesophilic
67770positivegrowth37mesophilic
119640positivegrowth25-41
119640nogrowth10psychrophilic
119640nogrowth15psychrophilic
119640nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23186facultative anaerobe
58074microaerophile
119640facultative anaerobe

spore formation

  • @ref: 23186
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
119640NaClpositivegrowth0-6 %
119640NaClnogrowth8 %
119640NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2318622599arabinose-builds acid from
2318617057cellobiose-builds acid from
2318617754glycerol-builds acid from
2318628087glycogen-builds acid from
2318615443inulin-builds acid from
2318617716lactose-builds acid from
2318617306maltose-builds acid from
2318629864mannitol-builds acid from
2318616634raffinose-builds acid from
2318626546rhamnose-builds acid from
2318633942ribose-builds acid from
2318617814salicin-builds acid from
2318630911sorbitol-builds acid from
2318627082trehalose-builds acid from
2318618222xylose-builds acid from
2318627897tryptophan-energy source
231864853esculin-hydrolysis
231865291gelatin-hydrolysis
2318628017starch-hydrolysis
2318617234glucose+builds acid from
2318617992sucrose+builds acid from
23186606565hippurate+hydrolysis
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11964016947citrate-carbon source
1196404853esculin-hydrolysis
119640606565hippurate-hydrolysis
11964017632nitrate-reduction
11964016301nitrite-reduction
11964045285pyrazinamide+hydrolysis
11964017632nitrate+respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 119640
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
2318635581indoleno
11964035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
2318635581indole-
11964015688acetoin-
11964017234glucose+

enzymes

@refvalueactivityec
23186acid phosphatase-3.1.3.2
23186alkaline phosphatase+3.1.3.1
23186alpha-fucosidase-3.2.1.51
23186alpha-galactosidase-3.2.1.22
23186alpha-glucosidase-3.2.1.20
23186alpha-mannosidase-3.2.1.24
23186arginine dihydrolase-3.5.3.6
23186beta-galactosidase-3.2.1.23
23186beta-glucosidase-3.2.1.21
23186beta-glucuronidase-3.2.1.31
23186catalase+1.11.1.6
23186chymotrypsin-3.4.4.5
23186cystine arylamidase-3.4.11.3
23186esterase (C 4)-
23186esterase lipase (C 8)+
23186leucine arylamidase+3.4.11.1
23186lipase (C 14)-
23186N-acetyl-beta-glucosaminidase-3.2.1.52
23186naphthol-AS-BI-phosphohydrolase+
23186nitrate reductase-1.7.99.4
23186pyrazinamidase+3.5.1.B15
23186pyrrolidonyl arylamidase-3.4.19.3
23186trypsin-3.4.21.4
23186tryptophan deaminase-4.1.99.1
23186urease-3.5.1.5
23186valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
119640oxidase-
119640beta-galactosidase-3.2.1.23
119640alcohol dehydrogenase-1.1.1.1
119640gelatinase-
119640amylase+
119640DNase-
119640caseinase-3.4.21.50
119640catalase+1.11.1.6
119640tween esterase+
119640gamma-glutamyltransferase-2.3.2.2
119640lecithinase-
119640lipase-
119640lysine decarboxylase-4.1.1.18
119640ornithine decarboxylase-4.1.1.17
119640phenylalanine ammonia-lyase-4.3.1.24
119640protease+
119640tryptophan deaminase-
119640urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20068---+-------+-------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20068+++-+----++--------
119640+++-+----++---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119640----+/------+++/------------------+----------+/-------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119640++-----------------+----------------------------++-------------------------+---+--------------+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11736clinical material (cosemetic dye)GermanyDEUEurope
58074Cosmetic dye
67770Cosmetic dye
119640Clinical materialGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial
#Infection#Medical environment#Clinic

taxonmaps

  • @ref: 69479
  • File name: preview.99_19810.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1654;97_2278;98_14967;99_19810&stattab=map
  • Last taxonomy: Corynebacterium glaucum subclade
  • 16S sequence: AJ431634
  • Sequence Identity:
  • Total samples: 447
  • soil counts: 2
  • aquatic counts: 21
  • animal counts: 420
  • plant counts: 4

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
117361Risk group (German classification)
200682German classification
1196402Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium glaucum 16S ribosomal RNA gene, partial sequenceAY438057419ena187491
11736Corynebacterium glaucum partial 16S rRNA gene, strain IMMIB R-5091TAJ4316341466ena187491

GC content

@refGC-contentmethod
1173664.3
2318663.6-65.0high performance liquid chromatography (HPLC)
6777063.6-65high performance liquid chromatography (HPLC)

External links

@ref: 11736

culture collection no.: DSM 44530, CIP 107989, NRRL B-24142, CCUG 48297, JCM 12208, IMMIB R-5091

straininfo link

  • @ref: 72712
  • straininfo: 100615

literature

  • topic: Phylogeny
  • Pubmed-ID: 12807190
  • title: Corynebacterium glaucum sp. nov.
  • authors: Yassin AF, Kroppenstedt RM, Ludwig W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02394-0
  • year: 2003
  • mesh: Bacterial Typing Techniques, *Coloring Agents, Corynebacterium/chemistry/*classification/genetics, *Cosmetics, DNA, Ribosomal/analysis, Molecular Sequence Data, Mycolic Acids/analysis, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11736Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44530)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44530
20068Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44530.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23186A. F. Yassin, R. M. Kroppenstedt, W. Ludwig10.1099/ijs.0.02394-0Corynebacterium glaucum sp. nov.IJSEM 53: 705-709 200312807190
38249Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5560
58074Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48297)https://www.ccug.se/strain?id=48297
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72712Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100615.1StrainInfo: A central database for resolving microbial strain identifiers
119640Curators of the CIPCollection of Institut Pasteur (CIP 107989)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107989