Strain identifier

BacDive ID: 3172

Type strain: Yes

Species: Corynebacterium propinquum

Strain Designation: Riegel B 77159, B 77159

Strain history: CIP <- 1993, H. Monteil, Strasbourg Hosp., Strasbourg, France: strain B 77159

NCBI tax ID(s): 1121367 (strain), 43769 (species)

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General

@ref: 11532

BacDive-ID: 3172

DSM-Number: 44285

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium propinquum Riegel B 77159 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from human respiratory tract.

NCBI tax id

NCBI tax idMatching level
43769species
1121367strain

strain history

@refhistory
11532<- CCUG <- P. Riegel, ULP Strasbourg, B 77159
381561993, H. Monteil, Strasbourg Hosp., France: strain B 77159
67770CCUG 33048 <-- P. Riegel B 77159.
121963CIP <- 1993, H. Monteil, Strasbourg Hosp., Strasbourg, France: strain B 77159

doi: 10.13145/bacdive3172.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium propinquum
  • full scientific name: Corynebacterium propinquum Riegel et al. 1994

@ref: 11532

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium propinquum

full scientific name: Corynebacterium propinquum Riegel et al. 1994 emend. Bernard et al. 2013

strain designation: Riegel B 77159, B 77159

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23176positiverod-shapedno
121963positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
19644Beige10-14 daysISP 2
19644Beige10-14 daysISP 3
19644Beige10-14 daysISP 4
19644Beige10-14 daysISP 5
19644Beige10-14 daysISP 6
19644Beige10-14 daysISP 7
231761 day

multicellular morphology

@refforms multicellular complexmedium name
19644noISP 2
19644noISP 3
19644noISP 4
19644noISP 5
19644noISP 6
19644noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11532TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535; with strain-specific modifications) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Tween 80 2.0 g/l Distilled water
11532TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19644ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19644ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19644ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19644ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19644ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19644ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38156MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
121963CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
121963CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
19644positiveoptimum37mesophilic
11532positivegrowth37mesophilic
23176positivegrowth25-37mesophilic
23176nogrowth42thermophilic
38156positivegrowth37mesophilic
67770positivegrowth37mesophilic
121963positivegrowth37mesophilic
121963nogrowth5psychrophilic
121963nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121963
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 121963
  • spore formation: no

halophily

  • @ref: 121963
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2317617632nitrate-reduction
1219634853esculin-hydrolysis
12196317632nitrate+reduction
12196316301nitrite-reduction
12196317632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 121963
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121963
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12196315688acetoin-
12196317234glucose-

enzymes

@refvalueactivityec
23176catalase+1.11.1.6
23176cytochrome oxidase-1.9.3.1
23176pyrazinamidase+3.5.1.B15
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
121963oxidase-
121963beta-galactosidase-3.2.1.23
121963alcohol dehydrogenase-1.1.1.1
121963gelatinase-
121963amylase-
121963catalase+1.11.1.6
121963lipase-
121963lysine decarboxylase-4.1.1.18
121963ornithine decarboxylase-4.1.1.17
121963urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19644---------+-------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19644-++-++---++--------
121963++++++---+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11532human respiratory tractStrasbourgFranceFRAEurope
23176Respiratory tract
51349Human tracheal aspirationStrasbourgFranceFRAEurope
67770Human respiratory tract
121963Human, Tracheal aspirationStrasbourgFranceFRAEurope1987

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_4082.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1164;97_1368;98_1659;99_4082&stattab=map
  • Last taxonomy: Corynebacterium propinquum
  • 16S sequence: X81917
  • Sequence Identity:
  • Total samples: 47846
  • soil counts: 2082
  • aquatic counts: 2462
  • animal counts: 42880
  • plant counts: 422

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115322Risk group (German classification)
196442Risk group (german classification)
1219632Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium propinquum strain DSM 44285 16S-23S ribosomal RNA intergenic spacer, complete sequenceAF536506389ena1121367
20218C.propinquum 16S rRNA gene (CIP 103792T)X819171396ena1121367
11532Corynebacterium propinquum partial 16S rRNA gene, strain CIP 103792X844381443ena1121367
67770Corynebacterium propinquum culture-collection NML:91-0122 16S ribosomal RNA gene, partial sequenceJX4760641444ena43769

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium propinquum DSM 442851121367.3wgspatric1121367
66792Corynebacterium propinquum strain FDAARGOS_111243769.22completepatric43769
66792Corynebacterium propinquum DSM 442852515154156draftimg1121367
67770Corynebacterium propinquum DSM 44285GCA_000375525contigncbi1121367

GC content

  • @ref: 67770
  • GC-content: 57
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes93.938yes
anaerobicno99.422no
halophileyes89.966no
spore-formingno93.956no
glucose-utilyes83.358no
aerobicyes60.573no
flagellatedno97.557yes
thermophileno93.641no
motileno95.24no
glucose-fermentno64.078yes

External links

@ref: 11532

culture collection no.: JCM 12106, DSM 44285, ATCC 51488, CCUG 33048, CIP 103792, LMG 19069

straininfo link

  • @ref: 72688
  • straininfo: 13102

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11532Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44285)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44285
19644Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44285.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23176Kathryn Bernard, Ana Luisa Pacheco, Ian Cunningham, Navdeep Gill, Tamara Burdz, Deborah Wiebe10.1099/ijs.0.046979-0Emendation of the description of the species Corynebacterium propinquum to include strains which produce ureaseIJSEM 63: 2146-2154 201323104363
38156Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15628
51349Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33048)https://www.ccug.se/strain?id=33048
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72688Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13102.1StrainInfo: A central database for resolving microbial strain identifiers
121963Curators of the CIPCollection of Institut Pasteur (CIP 103792)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103792