Strain identifier

BacDive ID: 3171

Type strain: Yes

Species: Corynebacterium macginleyi

Strain Designation: JCP 2, JCL-2

Strain history: CIP <- 1994, P. Riegel, Louis Pasteur Univ., Strasbourg, France: strain JCL-2

NCBI tax ID(s): 38290 (species)

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General

@ref: 11531

BacDive-ID: 3171

DSM-Number: 44284

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium macginleyi JCP 2 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from human corneal ulcer.

NCBI tax id

  • NCBI tax id: 38290
  • Matching level: species

strain history

@refhistory
11531<- CCUG <- P. Riegel, JCL-2
67770CCUG 32361 <-- P. Riegel JCL-2.
120736CIP <- 1994, P. Riegel, Louis Pasteur Univ., Strasbourg, France: strain JCL-2

doi: 10.13145/bacdive3171.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium macginleyi
  • full scientific name: Corynebacterium macginleyi Riegel et al. 1995

@ref: 11531

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium macginleyi

full scientific name: Corynebacterium macginleyi Riegel et al. 1995

strain designation: JCP 2, JCL-2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23175positiverod-shapedno
120736positiveflask-shapedno

colony morphology

@refcolony colorincubation periodmedium usedcolony size
20041Colorless10-14 daysISP 2
20041Colorless10-14 daysISP 3
20041Colorless10-14 daysISP 4
20041Colorless10-14 daysISP 5
20041Colorless10-14 daysISP 6
20041Colorless10-14 daysISP 7
23175reddish-beige1% Tween 80 supplemented sheep blood agar2-4 mm
120736

multicellular morphology

@refmedium nameforms multicellular complex
20041ISP 2
20041ISP 3no
20041ISP 4no
20041ISP 5no
20041ISP 6no
20041ISP 7no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11531COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20041ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20041ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20041ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20041ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
20041ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20041ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
231751% Tween 80 supplemented sheep blood agaryes
38165MEDIUM 355 - for Corynebacterium genitaliumyesDistilled water make up to (1000.000 ml);Tween 80 (1.000 ml);Trypto casein soy agar (40.000 g)
120736CIP Medium 355yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=355

culture temp

@refgrowthtypetemperaturerange
20041positiveoptimum37mesophilic
11531positivegrowth37mesophilic
38165positivegrowth37mesophilic
50973positivegrowth37mesophilic
67770positivegrowth37mesophilic
120736positivegrowth25-37mesophilic
120736nogrowth10psychrophilic
120736nogrowth15psychrophilic
120736nogrowth41thermophilic
120736nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23175facultative anaerobe
50973microaerophile
120736facultative anaerobe

spore formation

  • @ref: 20041
  • spore formation: no

compound production

  • @ref: 11531
  • compound: acid

halophily

@refsaltgrowthtested relationconcentration
120736NaClpositivegrowth0-2 %
120736NaClnogrowth4 %
120736NaClnogrowth6 %
120736NaClnogrowth8 %
120736NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2317565327D-xylose-builds acid from
2317528087glycogen-builds acid from
2317517716lactose-builds acid from
2317517306maltose-builds acid from
2317527082trehalose-builds acid from
2317529016arginine-degradation
231755291gelatin-degradation
2317528017starch-degradation
2317518186tyrosine-degradation
2317516199urea-degradation
231754853esculin-hydrolysis
231755291gelatin-hydrolysis
2317528017starch-hydrolysis
2317518186tyrosine-hydrolysis
2317516199urea-hydrolysis
2317517634D-glucose+builds acid from
2317533942ribose+builds acid from
2317517992sucrose+builds acid from
2317516991dna+degradation
2317517632nitrate+reduction
2317516899D-mannitol+/-fermentation
23175606565hippurate+/-hydrolysis
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12073616947citrate-carbon source
1207364853esculin-hydrolysis
120736606565hippurate-hydrolysis
12073617632nitrate+reduction
12073616301nitrite-reduction
12073615792malonate-assimilation
12073617632nitrate+respiration
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837965327D-xylose-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 120736
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120736
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12073615688acetoin-
12073617234glucose-

enzymes

@refvalueactivityec
23175alkaline phosphatase+3.1.3.1
23175alpha-glucosidase-3.2.1.20
23175beta-galactosidase-3.2.1.23
23175beta-glucuronidase-3.2.1.31
23175catalase+1.11.1.6
23175cytochrome oxidase-1.9.3.1
23175N-acetyl-beta-glucosaminidase-3.2.1.52
23175pyrazinamidase-3.5.1.B15
23175pyrrolidonyl arylamidase-3.4.19.3
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
120736oxidase-
120736beta-galactosidase-3.2.1.23
120736alcohol dehydrogenase-1.1.1.1
120736gelatinase-
120736caseinase-3.4.21.50
120736catalase+1.11.1.6
120736tween esterase+
120736gamma-glutamyltransferase-2.3.2.2
120736lecithinase-
120736lipase-
120736lysine decarboxylase-4.1.1.18
120736ornithine decarboxylase-4.1.1.17
120736phenylalanine ammonia-lyase-4.3.1.24
120736protease-
120736tryptophan deaminase-
120736urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
20041---+---------------
11531+--+-------++-+/---+--+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20041+++------++--------
120736+++------++---------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120736+---+----++++----+---------+--+-----------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11531human corneal ulcerSarrebourgFranceFRAEurope
23175eye of a patient suffering from a corneal ulcer
50973Human eye,corneal ulcerSarrebourgFranceFRAEurope
67770Eye of a patient suffering from a corneal ulcerSarrebourgFranceFRAEurope
120736ConjunctivitisSarrebourgFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Infection#Inflammation
#Host#Human
#Host Body-Site#Organ#Eye

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115312Risk group (German classification)
200412German classification
1207362Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
23175Corynebacterium macginleyi 16S rDNAX804991393nuccore38290
11531Corynebacterium macginleyi partial 16S rRNA gene, strain CIP104099TAJ4393451505ena38290

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium macginleyi strain CCUG 3236138290.5wgspatric38290
66792Corynebacterium macginleyi strain FDAARGOS_119538290.38completepatric38290
66792Corynebacterium macginleyi strain NML 08021238290.7wgspatric38290
66792Corynebacterium macginleyi strain NML 12020538290.6wgspatric38290
66792Corynebacterium macginleyi CCUG 323612862073393draftimg38290
67770Corynebacterium macginleyi CCUG 32361GCA_003688935contigncbi38290

GC content

@refGC-contentmethod
1153158high performance liquid chromatography (HPLC)
2317558.0high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes95.477yes
anaerobicno99.098yes
halophileyes91.209no
spore-formingno95.455yes
glucose-utilyes87.677no
aerobicno84.774yes
motileno94.627yes
flagellatedno98.135yes
thermophileno95.409no
glucose-fermentyes76.891no

External links

@ref: 11531

culture collection no.: DSM 44284, ATCC 51787, CCUG 32361, CIP 104099, JCM 11684, JCL 2, JCM 10393, LMG 19066

straininfo link

  • @ref: 72687
  • straininfo: 12987

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7857793Genomic diversity and phylogenetic relationships among lipid-requiring diphtheroids from humans and characterization of Corynebacterium macginleyi sp. nov.Riegel P, Ruimy R, de Briel D, Prevost G, Jehl F, Christen R, Monteil HInt J Syst Bacteriol10.1099/00207713-45-1-1281995Base Sequence, Corynebacterium/*classification/genetics/metabolism, DNA, Bacterial/analysis, Humans, *Lipid Metabolism, Molecular Sequence Data, Phenotype, PhylogenyMetabolism
Genetics30574590Whole-Genome Sequences of Corynebacterium macginleyi CCUG 32361(T) and Clinical Isolates NML 080212 and NML 120205.Bernier AM, Bernard KMicrobiol Resour Announc10.1128/MRA.01506-182018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11531Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44284)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44284
20041Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44284.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23175P. RIEGEL, R. RUIMY, D. DE BRIEL, G. PRéVOST, F. JEHL, R. CHRISTEN, H. MONTEIL10.1099/00207713-45-1-128Genomic Diversity and Phylogenetic Relationships among Lipid-Requiring Diphtheroids from Humans and Characterization of Corynebacterium macginleyi sp. nov.IJSEM 45: 128-133 19957857793
38165Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15969
50973Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32361)https://www.ccug.se/strain?id=32361
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
72687Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12987.1StrainInfo: A central database for resolving microbial strain identifiers
120736Curators of the CIPCollection of Institut Pasteur (CIP 104099)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104099