Strain identifier
BacDive ID: 3166
Type strain:
Species: Corynebacterium accolens
Strain history: CIP <- 1996, P. Riegel, Louis Pasteur Univ., Strasbourg, France <- CCUG <- CNCTC <- Neubauer
NCBI tax ID(s): 525260 (strain), 38284 (species)
General
@ref: 11644
BacDive-ID: 3166
DSM-Number: 44278
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic
description: Corynebacterium accolens DSM 44278 is a microaerophile, mesophilic bacterium that was isolated from human cervix.
NCBI tax id
NCBI tax id | Matching level |
---|---|
38284 | species |
525260 | strain |
strain history
@ref | history |
---|---|
11644 | <- CCUG <- J. Sourek, CNCTC |
67770 | CNCTC Th 1/57. |
115988 | CIP <- 1996, P. Riegel, Louis Pasteur Univ., Strasbourg, France <- CCUG <- CNCTC <- Neubauer |
doi: 10.13145/bacdive3166.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Corynebacteriaceae
- genus: Corynebacterium
- species: Corynebacterium accolens
- full scientific name: Corynebacterium accolens Neubauer et al. 1991
@ref: 11644
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Corynebacteriaceae
genus: Corynebacterium
species: Corynebacterium accolens
full scientific name: Corynebacterium accolens Neubauer et al. 1991 emend. Nouioui et al. 2018
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 93.286 | ||
69480 | 100 | positive | ||
115988 | no | positive | rod-shaped |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19643 | Light ivory | 10-14 days | ISP 2 |
19643 | 10-14 days | ISP 3 | |
19643 | Light ivory | 10-14 days | ISP 7 |
115988 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19643 | no | ISP 2 |
19643 | no | ISP 3 |
19643 | no | ISP 4 |
19643 | no | ISP 5 |
19643 | no | ISP 6 |
19643 | no | ISP 7 |
multimedia
- @ref: 11644
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44278.jpg
- caption: Medium 693 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11644 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
11644 | TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) | yes | https://mediadive.dsmz.de/medium/535a | Name: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
19643 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19643 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19643 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
115988 | CIP Medium 4 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4 | |
115988 | CIP Medium 6 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19643 | positive | optimum | 37 | mesophilic |
11644 | positive | growth | 37 | mesophilic |
49372 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
115988 | positive | growth | 30-41 | |
115988 | no | growth | 10 | psychrophilic |
115988 | no | growth | 15 | psychrophilic |
115988 | no | growth | 25 | mesophilic |
115988 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 49372
- oxygen tolerance: microaerophile
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.995 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
19643 | NaCl | positive | maximum | 2.5 % |
115988 | NaCl | positive | growth | 0-6 % |
115988 | NaCl | no | growth | 8 % |
115988 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
22911 | 4853 | esculin | - | hydrolysis |
22911 | 17234 | glucose | + | builds acid from |
22911 | 17306 | maltose | + | builds acid from |
22911 | 33942 | ribose | + | builds acid from |
22911 | 17992 | sucrose | - | builds acid from |
22911 | 18222 | xylose | - | builds acid from |
22911 | 17632 | nitrate | + | reduction |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 18287 | L-fucose | - | builds acid from |
68371 | 28847 | D-fucose | - | builds acid from |
68371 | 16443 | D-tagatose | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | + | builds acid from |
68371 | 28066 | gentiobiose | - | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | + | builds acid from |
68371 | 28017 | starch | + | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | - | builds acid from |
68371 | 17992 | sucrose | - | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | - | builds acid from |
68371 | 17306 | maltose | + | builds acid from |
68371 | 17057 | cellobiose | - | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | - | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | + | builds acid from |
68371 | 15824 | D-fructose | + | builds acid from |
68371 | 17634 | D-glucose | + | builds acid from |
68371 | 12936 | D-galactose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | + | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | + | builds acid from |
115988 | 16947 | citrate | - | carbon source |
115988 | 4853 | esculin | - | hydrolysis |
115988 | 606565 | hippurate | - | hydrolysis |
115988 | 17632 | nitrate | + | reduction |
115988 | 16301 | nitrite | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
antibiotic resistance
- @ref: 115988
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
- @ref: 115988
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 115988
- Chebi-ID: 17234
- metabolite: glucose
- methylred-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
22911 | acid phosphatase | - | 3.1.3.2 |
22911 | alkaline phosphatase | - | 3.1.3.1 |
22911 | cystine arylamidase | - | 3.4.11.3 |
22911 | esterase (C 4) | +/- | |
22911 | esterase Lipase (C 8) | + | |
22911 | leucine arylamidase | + | 3.4.11.1 |
22911 | lipase (C 14) | + | |
22911 | pyrazinamidase | - | 3.5.1.B15 |
22911 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
22911 | trypsin | +/- | 3.4.21.4 |
22911 | valine arylamidase | - | |
22911 | urease | - | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68379 | catalase | + | 1.11.1.6 |
68379 | gelatinase | - | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
115988 | oxidase | - | |
115988 | beta-galactosidase | - | 3.2.1.23 |
115988 | alcohol dehydrogenase | - | 1.1.1.1 |
115988 | gelatinase | - | |
115988 | amylase | - | |
115988 | DNase | +/- | |
115988 | caseinase | - | 3.4.21.50 |
115988 | catalase | + | 1.11.1.6 |
115988 | tween esterase | +/- | |
115988 | gamma-glutamyltransferase | - | 2.3.2.2 |
115988 | lecithinase | - | |
115988 | lipase | - | |
115988 | lysine decarboxylase | - | 4.1.1.18 |
115988 | ornithine decarboxylase | - | 4.1.1.17 |
115988 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
115988 | tryptophan deaminase | - | |
115988 | urease | - | 3.5.1.5 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG | Control | CAT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19643 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | ||
11644 | + | - | + | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | + |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19643 | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
115988 | + | + | + | - | + | - | - | - | - | + | + | - | - | - | + | - | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
115988 | + | - | - | - | + | - | - | - | - | - | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | + | + | - | - | + | - | - | - | - | - | - | +/- | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
11644 | human cervix | Havlickuv, Brod | Czech Republic | CZE | Europe |
49372 | Human clinical specimen | Europe | |||
67770 | Human cervix | Czech Republic | CZE | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | #Female |
#Host Body-Site | #Urogenital tract | |
#Infection | #Patient |
taxonmaps
- @ref: 69479
- File name: preview.99_1494.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_322;97_358;98_897;99_1494&stattab=map
- Last taxonomy: Corynebacterium accolens subclade
- 16S sequence: X80500
- Sequence Identity:
- Total samples: 9102
- soil counts: 201
- aquatic counts: 330
- animal counts: 8517
- plant counts: 54
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11644 | 2 | Risk group (German classification) |
19643 | 2 | Risk group (German classification) |
115988 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Corynebacterium accolens 16S rDNA | X80500 | 1393 | ena | 38284 |
20218 | Corynebacterium accolens gene for 16S rRNA, partial sequence | AB470621 | 545 | ena | 38284 |
11644 | Corynebacterium accolens partial 16S rRNA gene, strain ATCC 49725T | AJ439346 | 1505 | ena | 38284 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Corynebacterium accolens ATCC 49725 | 525260.4 | wgs | patric | 525260 |
66792 | Corynebacterium accolens ATCC 49725 | 643886058 | draft | img | 525260 |
67770 | Corynebacterium accolens ATCC 49725 | GCA_000159115 | scaffold | ncbi | 525260 |
66792 | Corynebacterium accolens DSM 44278 | GCA_023520795 | complete | ncbi | 38284 |
GC content
- @ref: 67770
- GC-content: 59.7
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 98.048 | no |
gram-positive | yes | 95.13 | no |
anaerobic | no | 99.308 | yes |
aerobic | no | 81.406 | yes |
halophile | yes | 89.462 | no |
spore-forming | no | 95.521 | no |
motile | no | 94.444 | no |
glucose-ferment | yes | 78.828 | no |
thermophile | no | 94.521 | yes |
glucose-util | yes | 87.861 | no |
External links
@ref: 11644
culture collection no.: DSM 44278, ATCC 49725, CCUG 28779, CNCTC Th1/57, LMG 19042, JCM 8331, CGMCC 1.1940, CIP 104783, KCTC 3431
straininfo link
- @ref: 72682
- straininfo: 97597
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11644 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44278) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44278 | |||
19643 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44278.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
22911 | Vicky Merhej, Enevold Falsen, Didier Raoult, Véronique Roux | 10.1099/ijs.0.005827-0 | Corynebacterium timonense sp. nov. and Corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid | IJSEM 59: 1953-1959 2009 | 19567562 | |
49372 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 28779) | https://www.ccug.se/strain?id=28779 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
72682 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID97597.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
115988 | Curators of the CIP | Collection of Institut Pasteur (CIP 104783) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104783 |