Strain identifier

BacDive ID: 3163

Type strain: Yes

Species: Corynebacterium mucifaciens

Strain history: CIP <- 1997, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 2278

NCBI tax ID(s): 1161097 (strain), 57171 (species)

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General

@ref: 11637

BacDive-ID: 3163

DSM-Number: 44265

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Corynebacterium mucifaciens DSM 44265 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from human blood culture.

NCBI tax id

NCBI tax idMatching level
1161097strain
57171species

strain history

@refhistory
11637<- G. Funke , University of Zurich; DMMZ 2278
345291997, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 2278
67770CCUG 36878 <-- G. Funke DMMZ 2278.
121153CIP <- 1997, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 2278

doi: 10.13145/bacdive3163.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium mucifaciens
  • full scientific name: Corynebacterium mucifaciens Funke et al. 1997

@ref: 11637

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium mucifaciens

full scientific name: Corynebacterium mucifaciens Funke et al. 1997

type strain: yes

Morphology

cell morphology

@refgram stainmotilitycell shape
23173positiveno
121153positivenorod-shaped

colony morphology

@refcolony colorincubation periodmedium usedcolony sizecolony shape
20036Lemon yellow (1012)10-14 daysISP 2
20036Lemon yellow (1012)10-14 daysISP 3
20036Lemon yellow (1012)10-14 daysISP 4
20036Lemon yellow (1012)10-14 daysISP 5
20036Lemon yellow (1012)10-14 daysISP 6
20036Lemon yellow (1012)10-14 daysISP 7
23173slightly yellowish1 daysheep blood agar1.0-1.5 mmcircular
121153

multicellular morphology

@refmedium nameforms multicellular complex
20036ISP 2
20036ISP 3no
20036ISP 4no
20036ISP 5no
20036ISP 6no
20036ISP 7no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11637TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
11637COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20036ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20036ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20036ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20036ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20036ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20036ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
23173sheep blood agaryes
34529MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121153CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
20036positiveoptimum37mesophilic
11637positivegrowth37mesophilic
34529positivegrowth37mesophilic
53466positivegrowth37mesophilic
67770positivegrowth37mesophilic
121153positivegrowth25-41
121153nogrowth10psychrophilic
121153nogrowth15psychrophilic
121153nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53466aerobe
121153obligate aerobe

spore formation

@refspore formation
20036no
23173no

halophily

  • @ref: 121153
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23173168082-dehydro-D-gluconate-builds acid from
2317315963ribitol-builds acid from
2317327613amygdalin-builds acid from
2317322599arabinose-builds acid from
2317322605arabinitol-builds acid from
2317318305arbutin-builds acid from
2317362318D-lyxose-builds acid from
2317316634raffinose-builds acid from
2317316443D-tagatose-builds acid from
2317332528turanose-builds acid from
2317316813galactitol-builds acid from
2317317113erythritol-builds acid from
2317333984fucose-builds acid from
2317328260galactose-builds acid from
2317328066gentiobiose-builds acid from
2317324265gluconate-builds acid from
2317328087glycogen-builds acid from
2317317268myo-inositol-builds acid from
2317317266L-sorbose-builds acid from
2317317716lactose-builds acid from
2317317306maltose-builds acid from
2317329864mannitol-builds acid from
231736731melezitose-builds acid from
2317328053melibiose-builds acid from
23173320061methyl alpha-D-glucopyranoside-builds acid from
2317343943methyl alpha-D-mannoside-builds acid from
2317374863methyl beta-D-xylopyranoside-builds acid from
23173506227N-acetylglucosamine-builds acid from
2317326546rhamnose-builds acid from
2317317814salicin-builds acid from
2317330911sorbitol-builds acid from
2317327082trehalose-builds acid from
2317318222xylose-builds acid from
231734853esculin-hydrolysis
2317316199urea-hydrolysis
2317317632nitrate-reduction
2317328757fructose+builds acid from
2317317234glucose+builds acid from
2317317754glycerol+builds acid from
2317337684mannose+builds acid from
2317333942ribose+/-builds acid from
2317317992sucrose+/-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12115316947citrate-carbon source
1211534853esculin-hydrolysis
121153606565hippurate+hydrolysis
12115317632nitrate-reduction
12115316301nitrite-reduction
12115317632nitrate-respiration
683794853esculin-hydrolysis
683795291gelatin-hydrolysis
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121153
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12115315688acetoin-
12115317234glucose-

enzymes

@refvalueactivityec
23173acid phosphatase+3.1.3.2
23173alkaline phosphatase+3.1.3.1
23173alpha-fucosidase-3.2.1.51
23173alpha-galactosidase-3.2.1.22
23173alpha-glucosidase-3.2.1.20
23173alpha-mannosidase-3.2.1.24
23173beta-galactosidase-3.2.1.23
23173beta-glucosidase-3.2.1.21
23173beta-glucuronidase-3.2.1.31
23173catalase+1.11.1.6
23173chymotrypsin-3.4.4.5
23173cystine arylamidase+3.4.11.3
23173esterase+
23173esterase lipase (C 8)+
23173lipase-
23173N-acetyl-beta-glucosaminidase-3.2.1.52
23173phosphoamidase+/-3.9.1.1
23173pyrazinamidase+3.5.1.B15
23173pyrrolidonyl arylamidase+/-3.4.19.3
23173trypsin-3.4.21.4
23173valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
121153oxidase-
121153beta-galactosidase-3.2.1.23
121153alcohol dehydrogenase-1.1.1.1
121153gelatinase-
121153amylase-
121153DNase-
121153caseinase-3.4.21.50
121153catalase+1.11.1.6
121153tween esterase-
121153gamma-glutamyltransferase-2.3.2.2
121153lecithinase-
121153lipase-
121153lysine decarboxylase-4.1.1.18
121153ornithine decarboxylase-4.1.1.17
121153phenylalanine ammonia-lyase-4.3.1.24
121153tryptophan deaminase-
121153urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
20036+--+-----+-+-------
11637-+-+-------+/---------+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20036+++-+----++--------
121153+++-+----++---+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121153----+-----+++--------+-+++-+---+---+/-+------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121153+--------------------------------------------------------------------------+------+-----------+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
11637human blood cultureZürichSwitzerlandCHEEurope
23173Blood culture (cardiac surgery), male, 68 years1996
53466Human blood,cardiac surgeryZürichSwitzerlandCHEEurope1996
67770Blood culture (a 68 year-old man)ZurichSwitzerlandCHEEurope
121153Human, BloodZurichSwitzerlandCHEEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Infection#Patient#Blood culture
#Host#Human
#Host Body Product#Fluids#Blood
#Host#Human#Male

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
116372Risk group (German classification)
200362German classification
1211532Risk group (French classification)

Sequence information

16S sequences

  • @ref: 23173
  • description: Corynebacterium mucifaciens 16S rRNA gene, strain DMMZ 2278
  • accession: Y11200
  • length: 1459
  • database: nuccore
  • NCBI tax ID: 1161097

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium mucifaciens strain ATCC 70035557171.3wgspatric57171
66792Corynebacterium mucifaciens CIP 1051292512047048draftimg1161097
67770Corynebacterium mucifaciens ATCC 700355GCA_012396315contigncbi57171

GC content

@refGC-contentmethod
2317363.0-65.0high performance liquid chromatography (HPLC)
6777064high performance liquid chromatography (HPLC)

External links

@ref: 11637

culture collection no.: DSM 44265, CCUG 36878, CIP 105129, DMMZ 2278, JCM 10384, ATCC 700355, CCM 4678, LMG 19067

straininfo link

  • @ref: 72679
  • straininfo: 14167

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9336892Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material.Funke G, Lawson PA, Collins MDInt J Syst Bacteriol10.1099/00207713-47-4-9521997Corynebacterium/chemistry/*classification/*genetics/ultrastructure, Humans, Microbial Sensitivity Tests, Microscopy, Electron, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Bacterial/*analysis, RNA, Ribosomal, 16S/*analysis, Sequence Homology, Nucleic AcidPathogenicity
Phylogeny34846289Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces.Zhang G, Yang J, Lai XH, Jin D, Lu S, Ren Z, Qin T, Pu J, Ge Y, Cheng Y, Yang C, Lv X, Jiao Y, Huang Y, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0050692021Animals, Bacterial Typing Techniques, Base Composition, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Humans, *Marmota/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Trachea/microbiology, Vitamin K 2/analogs & derivatives/chemistryPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11637Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44265)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44265
20036Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44265.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23173Guido Funke, Paul A. Lawson, Matthew D. Collins10.1099/00207713-47-4-952Corynebacterium mucifaciens sp. nov., an Unusual Species from Human Clinical MaterialIJSEM 47: 952-957 19979336892
34529Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17115
53466Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 36878)https://www.ccug.se/strain?id=36878
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
72679Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14167.1StrainInfo: A central database for resolving microbial strain identifiers
121153Curators of the CIPCollection of Institut Pasteur (CIP 105129)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105129