Strain identifier

BacDive ID: 3161

Type strain: Yes

Species: Corynebacterium argentoratense

Strain Designation: IBS B10697

Culture col. no.: DSM 44202, CIP 104296, CCUG 34893

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General

@ref: 11598

BacDive-ID: 3161

DSM-Number: 44202

keywords: facultative anaerobe, Bacteria, 16S sequence, genome sequence, mesophilic, gram-positive, rod-shaped

description: Corynebacterium argentoratense IBS B10697 is a facultative anaerobe, mesophilic, gram-positive bacterium that was isolated from human throat.

strain history

doi: 10.13145/bacdive3161.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium argentoratense
  • full scientific name: Corynebacterium argentoratense Riegel et al. 1995

@ref: 11598

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium argentoratense

full scientific name: Corynebacterium argentoratense Riegel et al. 1995 emend. Nouioui et al. 2018

strain designation: IBS B10697

type strain: yes

Morphology

cell morphology

  • @ref: 23172
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedtype of hemolysiscolony sizecolony shape
20017Ivory (1014)10-14 daysISP 2
20017Ivory (1014)10-14 daysISP 3
20017Ivory (1014)10-14 daysISP 4
20017Ivory (1014)10-14 daysISP 5
20017Ivory (1014)10-14 daysISP 6
20017Ivory (1014)10-14 daysISP 7
23172creamy2 daysblood agargamma2 mmcircular

multicellular morphology

@refforms multicellular complexmedium name
20017noISP 2
20017noISP 3
20017noISP 4
20017noISP 5
20017noISP 6
20017noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11598COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
11598TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
20017ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20017ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20017ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20017ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20017ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20017ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
23172blood agaryes
41101MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
20017positiveoptimum28mesophilic
11598positivegrowth28mesophilic
41101positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 23172
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 23172
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2317216899D-mannitol-builds acid from
2317265327D-xylose-builds acid from
2317228260galactose-builds acid from
2317228087glycogen-builds acid from
2317217716lactose-builds acid from
2317217306maltose-builds acid from
2317217992sucrose-builds acid from
2317227082trehalose-builds acid from
2317216991dna-degradation
231725291gelatin-degradation
2317218186tyrosine-degradation
2317216199urea-degradation
2317216991dna-hydrolysis
231724853esculin-hydrolysis
231725291gelatin-hydrolysis
2317218186tyrosine-hydrolysis
2317216199urea-hydrolysis
2317217634D-glucose+builds acid from
2317228757fructose+builds acid from
2317217632nitrate+reduction
2317233942ribose+/-builds acid from
23172606565hippurate+/-hydrolysis
2317228017starch+/-hydrolysis

enzymes

@refvalueactivityec
231726-phospho-beta-galactosidase-3.2.1.85
23172acid phosphatase+/-3.1.3.2
23172alkaline phosphatase-3.1.3.1
23172alpha-chymotrypsin+3.4.21.1
23172alpha-glucosidase-3.2.1.20
23172beta-glucuronidase-3.2.1.31
23172catalase+1.11.1.6
23172cystine arylamidase+3.4.11.3
23172cytochrome oxidase-1.9.3.1
23172esterase+/-
23172esterase lipase (C 8)+
23172leucine arylamidase+/-3.4.11.1
23172N-acetyl-beta-glucosaminidase-3.2.1.52
23172pyrazinamidase+3.5.1.B15
23172pyrrolidonyl arylamidase-3.4.19.3

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
20017-----------+-------
11598-+-+-------++-------+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20017-++-+----+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11598human throatStrasbourgFranceFRAEurope
23172throats of four human patients
52389Human throat,tonsillitisStrasbourgFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human
#Host Body-Site#Oral cavity and airways#Throat

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115982Risk group (German classification)
200172German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium argentoratense strain DSM 44202 16S-23S ribosomal RNA intergenic spacer, complete sequenceAF536500337ena42817
11598C.argentoratense 16S rRNA gene (CIP 104296)X839551314ena42817

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium argentoratense DSM 44202GCA_000590555completencbi1348662
66792Corynebacterium argentoratense CNM 601/08GCA_000971875contigncbi42817
66792Corynebacterium argentoratense CNM 463/05GCA_000971865contigncbi42817
66792Corynebacterium argentoratense DSM 442021348662.3completepatric1348662
66792Corynebacterium argentoratense strain CNM 463/0542817.7wgspatric42817
66792Corynebacterium argentoratense strain CNM 601/0842817.8wgspatric42817

GC content

@refGC-contentmethod
1159860-61high performance liquid chromatography (HPLC)
2317260.0-61.0high performance liquid chromatography (HPLC)

External links

@ref: 11598

culture collection no.: DSM 44202, CIP 104296, CCUG 34893

straininfo link

@refpassport
20218http://www.straininfo.net/strains/48885
20218http://www.straininfo.net/strains/48884

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11598Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44202)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44202
20017Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44202.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23172P. RIEGEL, R. RUIMY, D. DE BRIEL, G. PREVOST, F. JEHL, F. BIMET, R. CHRISTEN, H. MONTEIL10.1099/00207713-45-3-533Corynebacterium argentoratense sp. nov., from the Human ThroatIJSEM 45: 533-537 1995
41101Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16188
52389Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34893)https://www.ccug.se/strain?id=34893
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)