Strain identifier

BacDive ID: 3158

Type strain: Yes

Species: Corynebacterium auris

Strain history: CIP <- 1995, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 328T

NCBI tax ID(s): 44750 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11417

BacDive-ID: 3158

DSM-Number: 44122

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium auris DSM 44122 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from patient with otitis media.

NCBI tax id

  • NCBI tax id: 44750
  • Matching level: species

strain history

@refhistory
11417<- G. Funke, 328
385121995, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 328T
67770CIP 104632 <-- G. Funke DMMZ 328.
116638CIP <- 1995, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain DMMZ 328T

doi: 10.13145/bacdive3158.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium auris
  • full scientific name: Corynebacterium auris Funke et al. 1995

@ref: 11417

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium auris

full scientific name: Corynebacterium auris Funke et al. 1995

type strain: yes

Morphology

cell morphology

  • @ref: 116638
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19630Sand yellow10-14 daysISP 2
19630Sand yellow10-14 daysISP 3
19630Sand yellow10-14 daysISP 4
19630Sand yellow10-14 daysISP 5
19630Sand yellow10-14 daysISP 6
19630Sand yellow10-14 daysISP 7
116638

multicellular morphology

@refmedium nameforms multicellular complex
19630ISP 2
19630ISP 3no
19630ISP 4no
19630ISP 5no
19630ISP 6no
19630ISP 7no

multimedia

@refmultimedia contentintellectual property rightscaption
11417https://www.dsmz.de/microorganisms/photos/DSM_44122-1.jpg© Leibniz-Institut DSMZ
11417https://www.dsmz.de/microorganisms/photos/DSM_44122.jpg© Leibniz-Institut DSMZMedium 535 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11417TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11417TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19630ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19630ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19630ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19630ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19630ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19630ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38512MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116638CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116638CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
116638CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
19630positiveoptimum37mesophilic
11417positivegrowth37mesophilic
38512positivegrowth37mesophilic
67770positivegrowth37mesophilic
116638positivegrowth25-41
116638nogrowth10psychrophilic
116638nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
51514microaerophile
116638obligate aerobe

spore formation

  • @ref: 19630
  • spore formation: no

halophily

  • @ref: 116638
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11663816947citrate-carbon source
1166384853esculin-hydrolysis
116638606565hippurate+hydrolysis
11663817632nitrate-reduction
11663816301nitrite-reduction
11663845285pyrazinamide+hydrolysis
11663817632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 116638
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116638
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11663815688acetoin-
11663817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116638oxidase-
116638beta-galactosidase+3.2.1.23
116638alcohol dehydrogenase-1.1.1.1
116638gelatinase-
116638amylase-
116638DNase+
116638caseinase-3.4.21.50
116638catalase+1.11.1.6
116638tween esterase+
116638gamma-glutamyltransferase+2.3.2.2
116638lecithinase-
116638lipase-
116638lysine decarboxylase-4.1.1.18
116638ornithine decarboxylase-4.1.1.17
116638phenylalanine ammonia-lyase-4.3.1.24
116638protease-
116638tryptophan deaminase-
116638urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    51514C14:00.514
    51514C15:01115
    51514C16:011.516
    51514C17:013.317
    51514C18:05.218
    51514C15:1 ω8c2.314.792
    51514C16:1 ω9c0.815.774
    51514C17:0 anteiso3.216.722
    51514C17:1 ISO I/C16:0 DMA0.416.481
    51514C17:1 ω8c27.816.792
    51514C18:1 ω9c22.117.769
    51514C18:2 ω6,9c/C18:0 ANTE1.717.724
    51514Unidentified0.413.236
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19630---+-----+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19630+++-+----+---------
116638+++++----++---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116638-------------------------------------------------+-------------------------+++-++-------+-+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11417patient with otitis mediaZürichSwitzerlandCHEEurope
51514Human otitis mediaZürichSwitzerlandCHEEurope
67770Patient with ear infectionZurichSwitzerlandCHEEurope
116638Human, Ear infectionZurichSwitzerlandCHEEurope1993

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body-Site#Organ#Ear

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
114172Risk group (German classification)
196302Risk group (German classification)
1166382Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium auris 16S ribosomal RNA gene, partial sequenceAY438048419ena44750
20218Corynebacterium auris 16S rRNA, strain DZZM 328X818731329ena44750
20218Corynebacterium auris gene for 16S rRNA, partial sequenceAB470618544ena44750

GC content

@refGC-contentmethod
1141770
6777070high performance liquid chromatography (HPLC)

External links

@ref: 11417

culture collection no.: DSM 44122, CCUG 33426, JCM 11946, ATCC 51966, CCM 4566, CIP 104632, LMG 19072, DMMZ 328T

straininfo link

  • @ref: 72674
  • straininfo: 13114

literature

  • topic: Phylogeny
  • Pubmed-ID: 7547292
  • title: Heterogeneity within human-derived centers for disease control and prevention (CDC) coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov.
  • authors: Funke G, Lawson PA, Collins MD
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-45-4-735
  • year: 1995
  • mesh: Actinomycetales/*classification, Base Composition, Base Sequence, Centers for Disease Control and Prevention, U.S., Corynebacterium/*classification, DNA, Ribosomal/chemistry, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, United States
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11417Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44122)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44122
19630Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44122.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38512Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16562
51514Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33426)https://www.ccug.se/strain?id=33426
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
72674Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13114.1StrainInfo: A central database for resolving microbial strain identifiers
116638Curators of the CIPCollection of Institut Pasteur (CIP 104632)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104632