Strain identifier

BacDive ID: 3125

Type strain: No

Species: Corynebacterium variabile

Strain Designation: AC 256

Strain history: K. Suzuki CNF 057 <-- W. H. J. Crombach AC 256.

NCBI tax ID(s): 1727 (species)

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General

@ref: 8766

BacDive-ID: 3125

DSM-Number: 20536

keywords: 16S sequence, Bacteria, mesophilic

description: Corynebacterium variabile AC 256 is a mesophilic bacterium that was isolated from meshanger cheese.

NCBI tax id

  • NCBI tax id: 1727
  • Matching level: species

strain history

@refhistory
8766<- W.H.J. Crombach, AC 256 (Caseobacter polymorphus)
67770K. Suzuki CNF 057 <-- W. H. J. Crombach AC 256.

doi: 10.13145/bacdive3125.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium variabile
  • full scientific name: Corynebacterium variabile corrig. (Müller 1961) Collins 1987

  • synonyms

    @refsynonym
    20215Caseobacter polymorphus
    20215Arthrobacter variabilis
    20215Corynebacterium mooreparkense
    20215Corynebacterium variabilis

@ref: 8766

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium variabile

full scientific name: Corynebacterium variabile (Müller 1961) Collins 1987

strain designation: AC 256

type strain: no

Morphology

multimedia

  • @ref: 8766
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_20536.jpg
  • caption: Medium 535a 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8766TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
8766TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
8766positivegrowth28mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

murein

  • @ref: 8766
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47862C14:03.914
    47862C15:01.415
    47862C16:023.816
    47862C17:09.917
    47862C18:03.218
    47862C16:1 ω9c0.615.774
    47862C17:0 anteiso5.416.722
    47862C17:1 ω5c ISO0.616.461
    47862C17:1 ω9c18.716.773
    47862C18:1 ω9c17.317.769
    47862C18:2 ω6,9c/C18:0 ANTE11.417.724
    47862C20:4 ω6,9,12,15c0.419.395
    47862TBSA 10Me18:03.518.392
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCATGLU
47862--------++---------+
47862--------++---------+-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
47862--++-+++--------+---

Isolation, sampling and environmental information

isolation

@refsample type
8766meshanger cheese
47862Meshanger cheese
67770Meshanger cheese

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_425.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_288;97_318;98_353;99_425&stattab=map
  • Last taxonomy: Corynebacterium
  • 16S sequence: AJ222816
  • Sequence Identity:
  • Total samples: 16580
  • soil counts: 1425
  • aquatic counts: 2885
  • animal counts: 11599
  • plant counts: 671

Safety information

risk assessment

  • @ref: 8766
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium variabilis (strain DSM 20536) 16S rRNA geneAJ2228161438ena1727
20218Corynebacterium variabilis 16S rRNA (part.)X531851458ena1727

GC content

@refGC-contentmethod
876666.1
6777063.9thermal denaturation, midpoint method (Tm)
6777066.1thermal denaturation, midpoint method (Tm)

External links

@ref: 8766

culture collection no.: DSM 20536, ATCC 33010, IFO 14757, NBRC 14757, NCDO 2097, CCUG 23851, CCM 3383, LMG 19059, JCM 1377, BCRC 14862, CECT 4164, IAM 12549, KCTC 9102

straininfo link

  • @ref: 72642
  • straininfo: 217648

literature

  • topic: Phylogeny
  • Pubmed-ID: 9721308
  • title: Intraspecific variation of unusual phospholipids from Corynebacterium spp. containing a novel fatty acid.
  • authors: Niepel T, Meyer H, Wray V, Abraham WR
  • journal: J Bacteriol
  • DOI: 10.1128/JB.180.17.4650-4657.1998
  • year: 1998
  • mesh: Corynebacterium/genetics/*metabolism, Fatty Acids/chemistry/*metabolism, Magnetic Resonance Spectroscopy, Phospholipids/chemistry/*metabolism, Phylogeny, Species Specificity, Spectrometry, Mass, Fast Atom Bombardment
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8766Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20536)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20536
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
47862Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 23851)https://www.ccug.se/strain?id=23851
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72642Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID217648.1StrainInfo: A central database for resolving microbial strain identifiers